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... The appropriate formation of specific RNA-  protein 
Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
Kaplan, Lee M.; Chung, Raymond T.
complexes regulates the normal synthesis, ...
... the capacity to obtain large amounts of  protein 
Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
Willis, Sharon H.; Peng, Charline; Leon, Manuel Ponce de; Nicola, Anthony V.; Rux, Ann H.; Cohen, Gary H.; Eisenberg, Roselyn J.
for their studies. ...
... these systems are based on the fusion of the  protein 
Procedures for the Analysis and Purification of His-Tagged Proteins
Procedures for the Analysis and Purification of His-Tagged Proteins
Kneusel, Richard E.; Crowe, Joanne; Wulbeck, Melanie; Ribbe, Joachim
of interest with a naturally occurrIng ...
... In many cases, the analysis of a specific  protein 
Application of TEV Protease in Protein Production
Application of TEV Protease in Protein Production
Polayes, Deborah A.; Parks, T. Dawn; Johnston, Stephen A.; Dougherty, William G.
is impeded by the inability to purify large ...
... can comprise more than 50% of the total cell  protein 
Expression and Purification of Recombinant Proteins Using the pET System
Expression and Purification of Recombinant Proteins Using the pET System
Mierendorf, Robert C.; Morris, Barbara B.; Hammer, Beth; Novy, Robert E.
after a few hours of induction. ...
... We describe here the application of the  protein 
The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A
The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A
Björnsson, Asgeir; Mottagui-Tabar, Salim; Isaksson, Leif A.
A reporter gene 3A′ for in vivo translation ...
... As this chapter deals with the  protein 
Prokaryotic Protein-Serine/Threonine Phosphatases
Prokaryotic Protein-Serine/Threonine Phosphatases
Kennelly, Peter J.
-serine/threonine phosphatases of prokaryotic ...
... help in obtaining crystals. Materials  Protein 
Crystallization of Macromolecules for Three-Dimensional Structure Determination
Crystallization of Macromolecules for Three-Dimensional Structure Determination
Luisi, Ben; Anderson, Marie; Hope, Graham
Preparation It is crucial that the ...
... compulsory technique in the study of specific  protein 
Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins
Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins
Harris, Mark
- ...
... equipment and only small quantities of the  protein 
Mobility Shift Assays
Mobility Shift Assays
Savery, Nigel J.; Busby, Stephen J. W.
to be studied, yet it can yield detailed ...
... The targeting of proteins, via precise  protein 
Co-Immunoprecipitation
Co-Immunoprecipitation
Anderson, Neil G.
- ...
... with an appreciation that regulated, stable  protein 
Protein–Protein Interactions in Signaling Cascades
Protein–Protein Interactions in Signaling Cascades
Mayer, Bruce J.
- ...
... Partial proteolysis of an undenatured  protein 
Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
Jamison, Robert S.; Newcomer, Marcia E.; Ong, David E.
is a wtdely used, powerful technique to ...
... the transgene encoding the heterologous  protein 
Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Napier, Johnathan A.; Richard, Gaelle; Shewry, Peter R.
in a Mendelian fashion, the seed of a ...
... One common theme in  protein 
Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring
Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring
Hausken, Zachary E.; Coghlan, Vincent M.; Scott, John D.
- ...
... acid to these proteins is myristoyl-CoA:  Protein 
Membrane Targeting via Protein N-Myristoylation
Membrane Targeting via Protein N-Myristoylation
McIlhinney, R. A. Jeffrey
N-myristoyltransferase (NMT; EC 2.3.1.97). ...
... The  protein 
Permeabilized Mammalian Cells as a System for Protein Synthesis
Permeabilized Mammalian Cells as a System for Protein Synthesis
Stapulionis, Romualdas; Deutscher, Murray P.
-synthesizing machinery is probably the most ...
... for the determination of the N-terminus of a  protein 
Protein Sequencing
Protein Sequencing
Smith, Bryan J.; Chapman, John R.
or peptide (1), and thts chemistry is still ...
... of the addition of sugar residues to the  protein 
Analysis of N- and O-Glycosylation of Plant Proteins
Analysis of N- and O-Glycosylation of Plant Proteins
Fitchette-Lainé, Anne-Catherine; Denmat, Lise-Anne; Lerouge, Patrice; Faye, Loïc
backbone, while the ...
... At present the  protein 
The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
Leong, Louis E. C.
expression systems used commonly by ...
... and some recent examples of its use for  protein 
Microcalorimetry of Protein-Protein Interactions
Microcalorimetry of Protein-Protein Interactions
Cooper, Alan
- ...
... fluorescence are monitored as the  protein 
Fluorometric Titration of the CRABPs
Fluorometric Titration of the CRABPs
Norris, Andrew W.; Li, Ellen
is titrated with aliquots of retinoid. ...
... with negative effects on recombinant  protein 
Stability of Recombinant Proteins in Plants
Stability of Recombinant Proteins in Plants
Michaud, Dominique; Vrain, Thierry C.; Gomord, Véronique; Faye, Loïc
yields. ...
... contributed to our current understanding of  protein 
Use of Pichia pastoris as a Model Eukaryotic System
Use of Pichia pastoris as a Model Eukaryotic System
Faber, Klaas Nico; Elgersma, Ype; Heyman, John A.; Koller, Antonius; Lüers, Georg H.; Nuttley, William M.; Terlecky, Stanley R.; Wenzel, Thibaut J.; Subramani, Suresh
targeting to, and biogenesis of, ...
... is an essential process in characterizing a  protein 
Protein Purification
Protein Purification
Conner, Joseph
of interest. ...