|... The appropriate formation of specific RNA-
|Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
Kaplan, Lee M.; Chung, Raymond T.
|complexes regulates the normal synthesis, ...
|... the capacity to obtain large amounts of
|Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
Willis, Sharon H.; Peng, Charline; Leon, Manuel Ponce de; Nicola, Anthony V.; Rux, Ann H.; Cohen, Gary H.; Eisenberg, Roselyn J.
|for their studies. ...
|... these systems are based on the fusion of the
|Procedures for the Analysis and Purification of His-Tagged Proteins
Procedures for the Analysis and Purification of His-Tagged Proteins
Kneusel, Richard E.; Crowe, Joanne; Wulbeck, Melanie; Ribbe, Joachim
|of interest with a naturally occurrIng ...
|... In many cases, the analysis of a specific
|Application of TEV Protease in Protein Production
Application of TEV Protease in Protein Production
Polayes, Deborah A.; Parks, T. Dawn; Johnston, Stephen A.; Dougherty, William G.
|is impeded by the inability to purify large ...
|... can comprise more than 50% of the total cell
|Expression and Purification of Recombinant Proteins Using the pET System
Expression and Purification of Recombinant Proteins Using the pET System
Mierendorf, Robert C.; Morris, Barbara B.; Hammer, Beth; Novy, Robert E.
|after a few hours of induction. ...
|... We describe here the application of the
|The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A
The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A
Björnsson, Asgeir; Mottagui-Tabar, Salim; Isaksson, Leif A.
|A reporter gene 3A′ for in vivo translation ...
|... As this chapter deals with the
|Prokaryotic Protein-Serine/Threonine Phosphatases
Prokaryotic Protein-Serine/Threonine Phosphatases
Kennelly, Peter J.
|-serine/threonine phosphatases of prokaryotic ...
|... help in obtaining crystals. Materials
|Crystallization of Macromolecules for Three-Dimensional Structure Determination
Crystallization of Macromolecules for Three-Dimensional Structure Determination
Luisi, Ben; Anderson, Marie; Hope, Graham
|Preparation It is crucial that the ...
|... compulsory technique in the study of specific
|Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins
Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins
|... equipment and only small quantities of the
|Mobility Shift Assays
Mobility Shift Assays
Savery, Nigel J.; Busby, Stephen J. W.
|to be studied, yet it can yield detailed ...
|... The targeting of proteins, via precise
Anderson, Neil G.
|... with an appreciation that regulated, stable
|Protein–Protein Interactions in Signaling Cascades
Protein–Protein Interactions in Signaling Cascades
Mayer, Bruce J.
|... Partial proteolysis of an undenatured
|Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
Jamison, Robert S.; Newcomer, Marcia E.; Ong, David E.
|is a wtdely used, powerful technique to ...
|... the transgene encoding the heterologous
|Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Napier, Johnathan A.; Richard, Gaelle; Shewry, Peter R.
|in a Mendelian fashion, the seed of a ...
|... One common theme in
|Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring
Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring
Hausken, Zachary E.; Coghlan, Vincent M.; Scott, John D.
|... acid to these proteins is myristoyl-CoA:
|Membrane Targeting via Protein N-Myristoylation
Membrane Targeting via Protein N-Myristoylation
McIlhinney, R. A. Jeffrey
|N-myristoyltransferase (NMT; EC 220.127.116.11). ...
|Permeabilized Mammalian Cells as a System for Protein Synthesis
Permeabilized Mammalian Cells as a System for Protein Synthesis
Stapulionis, Romualdas; Deutscher, Murray P.
|-synthesizing machinery is probably the most ...
|... for the determination of the N-terminus of a
Smith, Bryan J.; Chapman, John R.
|or peptide (1), and thts chemistry is still ...
|... of the addition of sugar residues to the
|Analysis of N- and O-Glycosylation of Plant Proteins
Analysis of N- and O-Glycosylation of Plant Proteins
Fitchette-Lainé, Anne-Catherine; Denmat, Lise-Anne; Lerouge, Patrice; Faye, Loïc
|backbone, while the ...
|... At present the
|The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
Leong, Louis E. C.
|expression systems used commonly by ...
|... and some recent examples of its use for
|Microcalorimetry of Protein-Protein Interactions
Microcalorimetry of Protein-Protein Interactions
|... fluorescence are monitored as the
|Fluorometric Titration of the CRABPs
Fluorometric Titration of the CRABPs
Norris, Andrew W.; Li, Ellen
|is titrated with aliquots of retinoid. ...
|... with negative effects on recombinant
|Stability of Recombinant Proteins in Plants
Stability of Recombinant Proteins in Plants
Michaud, Dominique; Vrain, Thierry C.; Gomord, Véronique; Faye, Loïc
|... contributed to our current understanding of
|Use of Pichia pastoris as a Model Eukaryotic System
Use of Pichia pastoris as a Model Eukaryotic System
Faber, Klaas Nico; Elgersma, Ype; Heyman, John A.; Koller, Antonius; Lüers, Georg H.; Nuttley, William M.; Terlecky, Stanley R.; Wenzel, Thibaut J.; Subramani, Suresh
|targeting to, and biogenesis of, ...
|... is an essential process in characterizing a
|of interest. ...