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... has been a dramatic increase in the number of  protein 
Crystallography in the Study of Protein-DNA Interactions
Crystallography in the Study of Protein-DNA Interactions
Brown, David G.; Freemont, Paul S.
-DNA cocrystal structures solved at atomic ...
... allows purification of tagged proteins or  protein 
One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
Crowe, Joanne; Masone, Brigitte Steude; Ribbe, Joachim
complexes from <1% to >95% homogeneity in ...
... question depends on the purpose for which the  protein 
General Strategies
General Strategies
Doonan, Shawn
is required. ...
... Indeed, certain features or residues of a  protein 
Overexpression, Isolation, and Crystallization of Proteins
Overexpression, Isolation, and Crystallization of Proteins
Skelly, Jane V.; Madden, C. Bernadette
that are believed to impede crystal formation ...
... The solubility of a particular  protein 
Bulk Purification by Fractional Precipitation
Bulk Purification by Fractional Precipitation
Doonan, Shawn
in aqueous solution depends on the solvent ...
... molecules. The interaction between the target  protein 
Affinity Chromatography
Affinity Chromatography
Cutler, Paul
and the matrix is not based on general ...
... often a need to store an isolated or purified  protein 
Storage of Pure Proteins
Storage of Pure Proteins
Fágáin, Ciarán Ó.
for varying periods of time. ...
... and gel bands may be detected by both M r and  protein 
Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
Akins, Robert E.; Tuan, Rocky S.
-specific activities. ...
... one of the most common is to digest the  protein 
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone, Kathryn L.; Williams, Kenneth R.
enzymatically with a relatively specific ...
... The secondary structure of a  protein 
Protein Secondary Structure Investigated by Electrospray Ionization
Protein Secondary Structure Investigated by Electrospray Ionization
Robinson, Carol V.
is defined as the spatial arrangement of ...
... and accurate method for the estimation of  protein 
The Bradford Method for Protein Quantitation
The Bradford Method for Protein Quantitation
Kruger, Nicholas J.
concentration is essential in many fields of ...
... Detergents or surfactants are widely used in  protein 
The Effect of Detergents on Proteins Analyzed by Electrospray Ionization
The Effect of Detergents on Proteins Analyzed by Electrospray Ionization
Ogorzalek Loo, Rachele R.; Dales, Natalie; Andrews, Philip C.
chemistry (1–3), principally to solubilize ...
... is one of the most versatile methods for  protein 
Electroelution of Proteins from Polyacrylamide Gels
Electroelution of Proteins from Polyacrylamide Gels
Jenö, Paul; Horst, Martin
separation. ...
... only two different types of ligand. One is  protein 
Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A
Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A
Kruger, Nicholas J.
A, which is either radiolabeled or conjugated ...
... derivatives provide a new class of selective  protein 
Antisense-Mediated Inhibition of Protein Synthesis
Antisense-Mediated Inhibition of Protein Synthesis
Brysch, Wolfgang; Rifai, Abdalla; Tischmeyer, Wolfgang; Schlingensiepen, Karl-Hermann
synthesis inhibitors. ...
... data for DNA cloning or confirmation of  protein 
Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
Fernandez, Joseph; Mische, Sheenah M.
identification. ...
... are recent implementations of copper-based  protein 
Copper Iodide Staining of Proteins on Solid Phases
Copper Iodide Staining of Proteins on Solid Phases
Root, Douglas D.; Wang, Kuan
assays, and are designed to quantitate ...
... Many of the most powerful methods of  protein 
Practical Column Chromatography
Practical Column Chromatography
Doonan, Shawn
fractionation involve the use of column ...
... mixture resulting from the digestion of a  protein 
The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting
The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting
Cottrell, John S.; Sutton, Chris W.
by an enzyme can provide a fingerprint that ...
... described for estimation of the amount of  protein 
Quantification of Proteins by Staining in Polyacrylamide Gels
Quantification of Proteins by Staining in Polyacrylamide Gels
Smith, Bryan John
present in a sample as described in Chapters ...
... to methodology to minimize the assault on the  protein 
Avoidance of Proteolysis in Extracts
Avoidance of Proteolysis in Extracts
Beynon, Robert J.; Oliver, Simon
of interest (1–4). ...
... in our laboratory, from saccharide-  protein 
Polysaccharicb-Conjugate Vaccines
Polysaccharicb-Conjugate Vaccines
Peeters, Carla C. A. M.; Lagerman, Patrick R.; Weers, Odo; Oomen, Lukas A.; Hoogerhout, Peter; Beurret, Michel; Poolman, Jan T.
to polysaccharide- ...
... of peptides and proteins (1,2). Its use in  protein 
Reversed-Phase Chromatography of Proteins
Reversed-Phase Chromatography of Proteins
Neville, Bill
isolation and purification may have reached a ...
... the reaction mixture will contain the labeled  protein 
Purification and Assessment of Quality of Radioiodinated Protein
Purification and Assessment of Quality of Radioiodinated Protein
Bailey, Graham S.
, unlabeled ...
... primary structures, then cleavage of each  protein 
Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Judd, Ralph C.
with a specific protease or chemical cleavage ...