|... has been a dramatic increase in the number of
|Crystallography in the Study of Protein-DNA Interactions
Crystallography in the Study of Protein-DNA Interactions
Brown, David G.; Freemont, Paul S.
|-DNA cocrystal structures solved at atomic ...
|... allows purification of tagged proteins or
|One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
Crowe, Joanne; Masone, Brigitte Steude; Ribbe, Joachim
|complexes from <1% to >95% homogeneity in ...
|... question depends on the purpose for which the
|is required. ...
|... Indeed, certain features or residues of a
|Overexpression, Isolation, and Crystallization of Proteins
Overexpression, Isolation, and Crystallization of Proteins
Skelly, Jane V.; Madden, C. Bernadette
|that are believed to impede crystal formation ...
|... The solubility of a particular
|Bulk Purification by Fractional Precipitation
Bulk Purification by Fractional Precipitation
|in aqueous solution depends on the solvent ...
|... molecules. The interaction between the target
|and the matrix is not based on general ...
|... often a need to store an isolated or purified
|Storage of Pure Proteins
Storage of Pure Proteins
Fágáin, Ciarán Ó.
|for varying periods of time. ...
|... and gel bands may be detected by both M r and
|Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
Akins, Robert E.; Tuan, Rocky S.
|-specific activities. ...
|... one of the most common is to digest the
|Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone, Kathryn L.; Williams, Kenneth R.
|enzymatically with a relatively specific ...
|... The secondary structure of a
|Protein Secondary Structure Investigated by Electrospray Ionization
Protein Secondary Structure Investigated by Electrospray Ionization
Robinson, Carol V.
|is defined as the spatial arrangement of ...
|... and accurate method for the estimation of
|The Bradford Method for Protein Quantitation
The Bradford Method for Protein Quantitation
Kruger, Nicholas J.
|concentration is essential in many fields of ...
|... Detergents or surfactants are widely used in
|The Effect of Detergents on Proteins Analyzed by Electrospray Ionization
The Effect of Detergents on Proteins Analyzed by Electrospray Ionization
Ogorzalek Loo, Rachele R.; Dales, Natalie; Andrews, Philip C.
|chemistry (1–3), principally to solubilize ...
|... is one of the most versatile methods for
|Electroelution of Proteins from Polyacrylamide Gels
Electroelution of Proteins from Polyacrylamide Gels
Jenö, Paul; Horst, Martin
|... only two different types of ligand. One is
|Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A
Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A
Kruger, Nicholas J.
|A, which is either radiolabeled or conjugated ...
|... derivatives provide a new class of selective
|Antisense-Mediated Inhibition of Protein Synthesis
Antisense-Mediated Inhibition of Protein Synthesis
Brysch, Wolfgang; Rifai, Abdalla; Tischmeyer, Wolfgang; Schlingensiepen, Karl-Hermann
|synthesis inhibitors. ...
|... data for DNA cloning or confirmation of
|Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
Fernandez, Joseph; Mische, Sheenah M.
|... are recent implementations of copper-based
|Copper Iodide Staining of Proteins on Solid Phases
Copper Iodide Staining of Proteins on Solid Phases
Root, Douglas D.; Wang, Kuan
|assays, and are designed to quantitate ...
|... Many of the most powerful methods of
|Practical Column Chromatography
Practical Column Chromatography
|fractionation involve the use of column ...
|... mixture resulting from the digestion of a
|The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting
The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting
Cottrell, John S.; Sutton, Chris W.
|by an enzyme can provide a fingerprint that ...
|... described for estimation of the amount of
|Quantification of Proteins by Staining in Polyacrylamide Gels
Quantification of Proteins by Staining in Polyacrylamide Gels
Smith, Bryan John
|present in a sample as described in Chapters ...
|... to methodology to minimize the assault on the
|Avoidance of Proteolysis in Extracts
Avoidance of Proteolysis in Extracts
Beynon, Robert J.; Oliver, Simon
|of interest (1–4). ...
|... in our laboratory, from saccharide-
Peeters, Carla C. A. M.; Lagerman, Patrick R.; Weers, Odo; Oomen, Lukas A.; Hoogerhout, Peter; Beurret, Michel; Poolman, Jan T.
|to polysaccharide- ...
|... of peptides and proteins (1,2). Its use in
|Reversed-Phase Chromatography of Proteins
Reversed-Phase Chromatography of Proteins
|isolation and purification may have reached a ...
|... the reaction mixture will contain the labeled
|Purification and Assessment of Quality of Radioiodinated Protein
Purification and Assessment of Quality of Radioiodinated Protein
Bailey, Graham S.
|, unlabeled ...
|... primary structures, then cleavage of each
|Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Judd, Ralph C.
|with a specific protease or chemical cleavage ...