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... μl) was performed with negatively supercoiled  pUC18 
A functional type I topoisomerase from Pseudomonas aeruginosa
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... of trimers) and a DNA substrate: i) undigested  pUC18 
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong; Watt, Rory M
(184 ng); ii) PstI-linearized ...
... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... materials For binding experiments plasmid DNA (  pUC18 
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan; Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... [0.7 % (w/v)] showing the cleavage of  pUC18 
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Mendo, Ana Soraia; Figueiredo, Sara; Roma-Rodrigues, Catarina; Videira, Paula A.; Ma, Zhen; Diniz, Mário; Larguinho, Miguel; Costa, Pedro M.; Lima, João C.; Pombeiro, Armando J. L.; Baptista, Pedro V.; Fernandes, Alexandra R.
DNA (200 ng) incubated with ZM5 (50 μM) in the ...
... putative oriT RA3 (Figure 1B) was cloned into  pUC18 
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
Godziszewska, Jolanta; Kulińska, Anna; Jagura-Burdzy, Grażyna
[23], it facilitated plasmid mobilization by ...
... − lacZ − was cotransformed with various PutA-  pUC18 
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.
constructs (PutA- ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong; Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... of the IPTG-inducible promoter, AAEC189(  pUC18 
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Dai, Jianjun; Wang, Shaohui; Guerlebeck, Doreen; Laturnus, Claudia; Guenther, Sebastian; Shi, Zhenyu; Lu, Chengping; Ewers, Christa
:aatA+P) expressing aatA under the control of ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... The libraries were developed in two vectors,  pUC18 
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Sharma, Nandita; Tanksale, Himgouri; Kapley, Atya; Purohit, Hemant J.
and pBeloBAC11, and efficacy was demonstrated ...
... expression profile of pIAAD3 merB in pQE30 and  pUC18 
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Murtaza, Imtiyaz; Dutt, Amit; Mushtaq, Dhuha; Ali, Arif
vectors each demonstrated 22.2 kDa proteins. ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... E. coli cloning vector, 4.3 kb TaKaRa     pUC18 
Role of DNA in Bacterial Aggregation
Role of DNA in Bacterial Aggregation
Liu, Hui-Hui; Yang, Yi-Ran; Shen, Xin-Cheng; Zhang, Zhi-Ling; Shen, Ping; Xie, Zhi-Xiong
E. coli cloning vector, 2.7 kb TaKaRa   Herring ...
... for AFM For AFM imaging in air, 100.0 μg/ml  pUC18 
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
Shen, Xin-Cheng; Bao, Lei; Zhang, Zhi-Ling; Liu, Xiaoshen; Pang, Dai-Wen; Xu, Jianrong
DNA sample was diluted into final concentration ...
... and inserted into the BamHI and SalI sites of  pUC18 
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Yong Kang, C.
. ...
... pSVJ21, was constructed by ligating p54 and  pUC18 
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Miteva, Vanya; Lantz, Sarah; Brenchley, Jean
and inserting a chloramphenicol acetyl ...
... into the SacI and SalI enzyme-cutting sites of  pUC18 
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Yari, Kheirollah; Afzali, Saboor; Mozafari, Hadi; Mansouri, Kamran; Mostafaie, Ali
cloning vector. ...