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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... provided by Prof. Gong Heng  K. pneumoniae/  pUC18 
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Zhao, Li; Zheng, Yu; Ma, Xingyuan; Wei, Dongzhi
K-dhaT Recombinant K. pneumoniae with plasmid ...
... in Escherichia coli JM109 from an inducible  pUC18 
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Huang, Yanna; You, Chunping; Liu, Zhenmin
vector, respectively, and the resulting strains ...
... by Shi et al. (2009a). The integrative vectors  pUC18 
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Wang, Guanglu; Bai, Ling; Wang, Zhiwen; Shi, Ting; Chen, Tao; Zhao, Xueming
-TPA, ...
... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... materials For binding experiments plasmid DNA (  pUC18 
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... erm This study Plasmids    pUC18 
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Fu, Jing; Huo, Guangxin; Feng, Lili; Mao, Yufeng; Wang, Zhiwen; Ma, Hongwu; Chen, Tao; Zhao, Xueming
Ampr Lab Stock   ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan; Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... by PCR. They were inserted into vector  pUC18 
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He; Hu, Nan
, yielding the recombinant expression vector ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... Culture Collection (CICC). The plasmid  pUC18 
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Guo, Xuewu; Wang, Yazhou; Guan, Xiangyu; Chen, Yefu; Zhang, Cuiying; Xiao, Dongguang
 K were provided by Yu Zheng, associate ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... from Novagen (Darmstadt, Germany). The plasmid  pUC18 
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Liu, D.; Schmid, R. D.; Rusnak, M.
was purchased from MBI Fermentas (St. ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... the lacZ-tagged strain B. subtilis Tu-100(  pUC18 
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Hu, Xiaojia; Roberts, Daniel P.; Jiang, Mulan; Zhang, Yinbo
) was detected within all roots of oilseed ...
... Mannheim, Germany). Subcloning into  pUC18 
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Debode, Frederic; Janssen, Eric; Berben, Gilbert
...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... feces (serotype O1483: H1) ATCC Plasmids    pUC18 
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Zhou, Lingyun; Liu, Qin; Wang, Qiyao; Ma, Yue; Xu, Yuzhou; Yang, Zhao; Zhao, Yan; Zhang, Yuanxing
Cloning/expression vector, Apr lacZ-α, Ori: ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong; Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... per recipient cell (t/r) using plasmid  pUC18 
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Rizzi, Aurora; Brusetti, Lorenzo; Arioli, Stefania; Nielsen, Kaare M.; Tamagnini, Isabella; Tamburini, Alberto; Sorlini, Claudia; Daffonchio, Daniele
or a 1,873 bp fragment as donor DNA, and 3.5 × ...
... expression profile of pIAAD3 merB in pQE30 and  pUC18 
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Murtaza, Imtiyaz; Dutt, Amit; Mushtaq, Dhuha; Ali, Arif
vectors each demonstrated 22.2 kDa proteins. ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...