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... by Shi et al. (2009a). The integrative vectors  pUC18 
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Wang, Guanglu; Bai, Ling; Wang, Zhiwen; Shi, Ting; Chen, Tao; Zhao, Xueming
-TPA, ...
... μl) was performed with negatively supercoiled  pUC18 
A functional type I topoisomerase from Pseudomonas aeruginosa
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... of trimers) and a DNA substrate: i) undigested  pUC18 
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong; Watt, Rory M
(184 ng); ii) PstI-linearized ...
... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... materials For binding experiments plasmid DNA (  pUC18 
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan; Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... by PCR. They were inserted into vector  pUC18 
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He; Hu, Nan
, yielding the recombinant expression vector ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... [0.7 % (w/v)] showing the cleavage of  pUC18 
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Mendo, Ana Soraia; Figueiredo, Sara; Roma-Rodrigues, Catarina; Videira, Paula A.; Ma, Zhen; Diniz, Mário; Larguinho, Miguel; Costa, Pedro M.; Lima, João C.; Pombeiro, Armando J. L.; Baptista, Pedro V.; Fernandes, Alexandra R.
DNA (200 ng) incubated with ZM5 (50 μM) in the ...
... − lacZ − was cotransformed with various PutA-  pUC18 
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.
constructs (PutA- ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong; Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... with two XcmI cassettes was inserted into  pUC18 
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
Gu, Jingsong; Ye, Chunjiang
to construct an efficient XcmI T-vector parent ...
... by standard PCR techniques from the template  pUC18 
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Caly, Leon; Jans, David A.; Piller, Sabine C.
-NL4.3 [15]. ...
... to keep the acidity constant at pH 6.0. Plasmid  pUC18 
Biological assays and noncovalent interactions of pyridine-2-carbaldehyde thiosemicarbazonecopper(II) drugs with [poly(dA–dT)]2, [poly(dG–dC)]2, and calf thymus DNA
Biological assays and noncovalent interactions of pyridine-2-carbaldehyde thiosemicarbazonecopper(II) drugs with [poly(dA–dT)]2, [poly(dG–dC)]2, and calf thymus DNA
Ruiz, Rebeca; García, Begoña; Garcia-Tojal, Javier; Busto, Natalia; Ibeas, Saturnino; Leal, José M.; Martins, Célia; Gaspar, Jorge; Borrás, Joaquín; Gil-García, Rubén; González-Álvarez, Marta
(0.25 μg μL−1, 750 μM in nucleotides) in TE ...
... DNA in solution The interaction of HUTth with  pUC18 
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
Papageorgiou, Anna C.; Adam, Panagiotis S.; Stavros, Philemon; Nounesis, George; Meijers, Rob; Petratos, Kyriacos; Vorgias, Constantinos E.
plasmid DNA was performed by MST (Zillner et ...
... K12 and the recombinant strain E. coli JM101  pUC18 
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Sevcenco, Ana-Maria; Krijger, Gerard C.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Hagen, Wilfred R.; Hagedoorn, Peter-Leon
-azu [17] were used. ...
... et al. (1991a) pEBM2 8.0 pBL1 Kmr, lacZα  pUC18 
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Pátek, Miroslav; Nešvera, Jan
MCS Mobilizable Tauch et al. (1998) pMJ-Ab 5.2 ...
... expression Datsenko and Wanner (2000)  pUC18 
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
Yang, Ying; Yang, Yi; Ou, Bingming; Xia, Pengpeng; Zhou, Mingxu; Li, Luan; Zhu, Guoqiang
Ampr, cloning vector Lab stock ...
... respectively, and subcloned into the plasmid  pUC18 
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Yang, Xue-Peng; Zhong, Gui-Fang; Lin, Jin-Ping; Mao, Duo-Bin; Wei, Dong-Zhi
between the EcoRI–PstI sites downstream of the ...
... fragment was subcloned into the HindIII site of  pUC18 
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Noda, Shuhei; Miyazaki, Takaya; Miyoshi, Takanori; Miyake, Michiru; Okai, Naoko; Tanaka, Tsutomu; Ogino, Chiaki; Kondo, Akihiko
. ...
... 621H pGOX3, and E. coli cloning vector  pUC18 
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Zhang, Lin; Lin, Jinping; Ma, Yushu; Wei, Dongzhi; Sun, Ming
. ...
... relA1 Clontech, Heidelberg, Germany Plasmids  pUC18 
The response of Serratia marcescens JG to environmental changes by quorum sensing system
The response of Serratia marcescens JG to environmental changes by quorum sensing system
Sun, Shu-Jing; Liu, Hui-Jun; Weng, Cai-Hong; Lai, Chun-Fen; Ai, Liu-Ying; Liu, Yu-Chen; Zhu, Hu
pBR322-derived cloning vector, Lac+, Apr MBI ...