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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... provided by Prof. Gong Heng  K. pneumoniae/  pUC18 
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Zhao, Li; Zheng, Yu; Ma, Xingyuan; Wei, Dongzhi
K-dhaT Recombinant K. pneumoniae with plasmid ...
... by Shi et al. (2009a). The integrative vectors  pUC18 
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Wang, Guanglu; Bai, Ling; Wang, Zhiwen; Shi, Ting; Chen, Tao; Zhao, Xueming
-TPA, ...
... nucleases were studied using supercoiled  pUC18 
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Hangan, Adriana Corina; Stan, Roxana Liana; Turza, Alexandru; Oprean, Luminiţa Simona; Páll, Emöke; Gheorghe-Cetean, Sînziana; Sevastre, Bogdan
DNA in cacodylate buffer (0.1 M, pH 6.0), in ...
... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... materials For binding experiments plasmid DNA (  pUC18 
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan; Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... by PCR. They were inserted into vector  pUC18 
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He; Hu, Nan
, yielding the recombinant expression vector ...
... A fragment of the DNA sequence of the  pUC18 
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Golubev, S. S.; Kononogov, S. A.; Ravin, N. V.; Skryabin, K. G.
plasmid is proposed as a reference sequence for ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... Culture Collection (CICC). The plasmid  pUC18 
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Guo, Xuewu; Wang, Yazhou; Guan, Xiangyu; Chen, Yefu; Zhang, Cuiying; Xiao, Dongguang
 K were provided by Yu Zheng, associate ...
... [0.7 % (w/v)] showing the cleavage of  pUC18 
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Mendo, Ana Soraia; Figueiredo, Sara; Roma-Rodrigues, Catarina; Videira, Paula A.; Ma, Zhen; Diniz, Mário; Larguinho, Miguel; Costa, Pedro M.; Lima, João C.; Pombeiro, Armando J. L.; Baptista, Pedro V.; Fernandes, Alexandra R.
DNA (200 ng) incubated with ZM5 (50 μM) in the ...
... on supercoiled covalently closed circular  pUC18 
Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions
Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions
Dizman, Yesim Akturk; Muratoglu, Hacer; Sandalli, Cemal; Nalcacioglu, Remziye; Demirbag, Zihni
DNA. ...
... methods Reagents and biochemicals The plasmid  pUC18 
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Perry, Christopher C.; Urata, Sarah M.; Lee, Melissa; Aguilera, Joe A.; Milligan, Jamie R.
was grown on a liter scale in an E. coli host ...
... − lacZ − was cotransformed with various PutA-  pUC18 
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.
constructs (PutA- ...
... from Novagen (Darmstadt, Germany). The plasmid  pUC18 
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Liu, D.; Schmid, R. D.; Rusnak, M.
was purchased from MBI Fermentas (St. ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... the lacZ-tagged strain B. subtilis Tu-100(  pUC18 
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Hu, Xiaojia; Roberts, Daniel P.; Jiang, Mulan; Zhang, Yinbo
) was detected within all roots of oilseed ...
... Mannheim, Germany). Subcloning into  pUC18 
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Debode, Frederic; Janssen, Eric; Berben, Gilbert
...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...