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... μl) was performed with negatively supercoiled  pUC18 
A functional type I topoisomerase from Pseudomonas aeruginosa
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... or characteristics Source or reference Plasmids  pUC18 
Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Feeney, Audrey; Johnston, Christopher D; Govender, Rodney; O’Mahony, Jim; Coffey, Aidan; Sleator, Roy D
Ampr, lacZ', pMB9 replicon [13] ...
... of trimers) and a DNA substrate: i) undigested  pUC18 
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong; Watt, Rory M
(184 ng); ii) PstI-linearized ...
... or characteristics Source or reference Plasmids  pUC18 
The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance
The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance
Feeney, Audrey; Johnston, Christopher D; Lucid, Alan; O’Mahony, Jim; Coffey, Aidan; Lucey, Brigid; Sleator, Roy D
Ampr, lacZ', pMB9 replicon [9] ...
... Knabc We constructed an expression vector from  pUC18 
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Kurz, Matthias; Brünig, Anika NS; Galinski, Erwin A
where the β-lactamase is replaced in-frame by ...
... the parasite’s DNA, an external control (  pUC18 
Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Gonçalves-de-Albuquerque, Suênia da Cunha; Pessoa e Silva, Rômulo; de Morais, Rayana Carla Silva; Trajano-Silva, Lays Adrianne Mendonça; Régis-da-Silva, Carlos Gustavo; Brandão-Filho, Sinval Pinto; de Paiva-Cavalcanti, Milena
) and an internal control (G3PD) for accurate ...
... erm This study Plasmids    pUC18 
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Fu, Jing; Huo, Guangxin; Feng, Lili; Mao, Yufeng; Wang, Zhiwen; Ma, Hongwu; Chen, Tao; Zhao, Xueming
Ampr Lab Stock   ...
... putative oriT RA3 (Figure 1B) was cloned into  pUC18 
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
Godziszewska, Jolanta; Kulińska, Anna; Jagura-Burdzy, Grażyna
[23], it facilitated plasmid mobilization by ...
... of the IPTG-inducible promoter, AAEC189(  pUC18 
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Dai, Jianjun; Wang, Shaohui; Guerlebeck, Doreen; Laturnus, Claudia; Guenther, Sebastian; Shi, Zhenyu; Lu, Chengping; Ewers, Christa
:aatA+P) expressing aatA under the control of ...
... expressions of DR4 and DR5, we transfected the  pUC18 
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
Kong, Fanyun; You, Hongjuan; Zhao, Jinjin; Liu, Wen; Hu, Lei; Luo, Wenya; Hu, Wei; Tang, Renxian; Zheng, Kuiyang
-HBV1.2, a plasmid contains the full-length HBV ...
... was conducted as previously described [17, 27].  pUC18 
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Jeong, Kwang Cheol; Hung, Kai Foong; Baumler, David J; Byrd, Jeffrey J; Kaspar, Charles W
plasmid DNA (300 ng) was mixed with varying ...
... antibody. Materials and methods Materials The  pUC18 
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Hao, Chao-hui; Han, Qian-he; Shan, Zhong-jie; Hu, Jian-ting; Zhang, Nan; Zhang, Xue-pei
and pSectag2B plasmids, restriction ...
... intergenic region (Fig. 1A) was cloned into  pUC18 
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Samsonova, Natalya N; Smirnov, Sergey V; Altman, Irina B; Ptitsyn, Leonid R
vector and sequenced. ...
... coli, such as those derived from pBR322 or  pUC18 
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
So, Lok Yan; Chen, Wen-yang; Lacap-Bugler, Donnabella C; Seemann, Myriam; Watt, Rory M
, cannot be stably-maintained in Z. ...
... showed no products with the gDNA of Z. mays and  pUC18 
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Weihmann, Fabian; Eisermann, Iris; Becher, Rayko; Krijger, Jorrit-Jan; Hübner, Konstantin; Deising, Holger B.; Wirsel, Stefan G. R.
(Additional file 1A). ...
...   pKD4 Apr Kmr [18]   pETSPO Cmr MLSr [4]     pUC18 
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Croux, Christian; Nguyen, Ngoc-Phuong-Thao; Lee, Jieun; Raynaud, Céline; Saint-Prix, Florence; Gonzalez-Pajuelo, Maria; Meynial-Salles, Isabelle; Soucaille, Philippe
Apr Fermentas    ...
... (carrying wildtype dnaQ), and the empty vector  pUC18 
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Luan, Guodong; Cai, Zhen; Li, Yin; Ma, Yanhe
, was transformed into E. coli cells, ...
... sterile and fertile lines were cloned into the  pUC18 
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
Das, Srirupa; Sen, Supriya; Chakraborty, Anirban; Chakraborti, Papia; Maiti, Mrinal K; Basu, Asitava; Basu, Debabrata; Sen, Soumitra K
vector. ...
... as previously described [2] with either:  pUC18 
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Lee, David J; Bingle, Lewis EH; Heurlier, Karin; Pallen, Mark J; Penn, Charles W; Busby, Stephen JW; Hobman, Jon L
[17], 2,700 bp (high copy number plasmid), ...
... below). Controls Nick-translated biotin-labeled  pUC18 
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Evans, Mark F; Aliesky, Holly A; Cooper, Kumarasen
plasmid was used as a control for non-specific ...
... In contrast, the high copy number plasmid  pUC18 
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Zhang, Junjie; De Masi, Leon; John, Beena; Chen, Wenxin; Schifferli, Dieter M
-OVA that constitutively expressed the ...
... CA, USA). Nuclease activity assay Plasmid  pUC18 
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Wu, Shih-Lu; Li, Chia-Cheng; Chen, Jaw-Chyun; Chen, Yi-Jin; Lin, Ching-Ting; Ho, Tin-Yun; Hsiang, Chien-Yun
dsDNA, preparing with the Qiagen plasmid midi ...
... and Rp107; Additional file 4) and the plasmid  pUC18 
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Hwang, Yeen Ting; McCartney, Andrew W; Gidda, Satinder K; Mullen, Robert T
/CIRV (purchased from the American Type Culture ...
... Plasmids pCC1FOS Cloning vector; Chlr Epicentre  pUC18 
Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
JunGang, Li; KeGui, Zhang; WenJun, Han
Cloning vector; Apr Takara pET28a Expression ...
... high copy number plasmids pLitmus38 (AMPr) and  pUC18 
Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level
Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level
Rohde, Alexander; Hammerl, Jens Andre; Al Dahouk, Sascha
(AMPr) or the medium copy number vector pBR328 ...

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