Snippet Search

Searching 12,248,340 documents.
Discover how a particular term or phrase is used in scientific literature

Showing 1 to 25 of 327 matching articles

Results per page:

... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... Culture Collection (CICC). The plasmid  pUC18 
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Guo, Xuewu; Wang, Yazhou; Guan, Xiangyu; Chen, Yefu; Zhang, Cuiying; Xiao, Dongguang
 K were provided by Yu Zheng, associate ...
... of the target sequence than those produced in  pUC18 
Sequencing Bacterial Artificial Chromosomes
Sequencing Bacterial Artificial Chromosomes
Harris, David E.; Murphy, Lee
. ...
... vector harboring the gene X to be mutated:  pUC18 
Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
Mondon, Philippe; Grand, David; Souyris, Nathalie; Emond, Stéphane; Bouayadi, Khalil; Kharrat, Hakim
-X (cloned into BamHI, EcoRI restriction ...
... Biotinylated oligo-d T18: 5′[biotin]dT18 3′. 8.  pUC18 
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Jansen, Bastiaan J. H.; Jongh, Gys; Schalkwijk, Joost; Ruissen, Fred
Forward: 5′ AAG TTG GGT AAC GCC AGG 3′. 9. ...
... and inserted into the BamHI and SalI sites of  pUC18 
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Yong Kang, C.
. ...
... the gene encoded in the plasmid substrates (  pUC18 
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan
: TS) for use in the cleavage reactions. (c) ...
... pDONR™ P4-P1R, and pDONR™ P2R-P3 (see Note 1),  pUC18 
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Sone, Takefumi; Imamoto, Fumio
(8), template DNA for amplification of ...
... et al. (1991a) pEBM2 8.0 pBL1 Kmr, lacZα  pUC18 
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Pátek, Miroslav; Nešvera, Jan
MCS Mobilizable Tauch et al. (1998) pMJ-Ab 5.2 ...
... and Methods Materials The 2686-bp-long  pUC18 
Evaluation of a Whole Genome Amplification Method Based on Improved Ligation-Mediated PCR
Evaluation of a Whole Genome Amplification Method Based on Improved Ligation-Mediated PCR
Pan, Xiaoming; Chen, Weikai; Jia, Xiushuang; Dong, Ping; Liang, Xingguo
plasmid was extracted from bacteria culture ...
... any plasmid encoding the LacZα-peptide such as  pUC18 
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Refaii, Abdalla Al; Jean-Hervé, Alix
/19 or pWSK129 (13) can be used. ...
... of the elongation factor 1α (EF 1α) promoter.  pUC18 
shRNA-Mediated Gene Knockdown in Skeletal Muscle
shRNA-Mediated Gene Knockdown in Skeletal Muscle
Golzio, Muriel; Escoffre, Jean-Michel; Teissié, Justin
plasmid contains the cDNA of subunit α of LacZ ...
... Preparation of Plasmid Vector 1. Plasmid  pUC18 
Monitoring Drug Resistance for Herpesviruses
Monitoring Drug Resistance for Herpesviruses
Andrei, Graciela; Fiten, Pierre; Clercq, Erik; Snoeck, Robert; Opdenakker, Ghislain
(Pharmacia Biotech). 2. BamHI restriction ...
... subcloned into a double-stranded vector such as  pUC18 
BAC Finishing Strategies
BAC Finishing Strategies
Bird, Christine; Grafham, Darren
or single-stranded M13 vector. ...
... pIJ101 aac(3)IV ermEp; oriT RK2 [16] pL98 7.4  pUC18 
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Rebets, Yuriy; Kormanec, Jan; Luzhetskyy, Andriy; Bernaerts, Kristel; Anné, Jozef
aac(3)IV pIJ101 aac(3)IV ssrAp; oriT RK2 [16] ...
... further cloning of the synthetic genes into  pUC18 
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Lamport, Derek T. A.; Tan, Li; Kieliszewski, Marcia J.
-SS tob -EGFP vector. ...
... vectors pUC18Not Cloning vector, with the  pUC18 
Genetic Analysis of Gram-Negative Bacteria Using Mini Tn5 Transposons
Genetic Analysis of Gram-Negative Bacteria Using Mini Tn5 Transposons
Cebolla*, A.; Arévalo-Rodríguez, M.
multicloning site flanked by NotI sites ApR, ...
... cloning of PCR products (Promega, Madison, WI);  pUC18 
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Zoulim, Fabien; Tong, ShuPing; Trépo, Christian
or pUC19 for whole HBV-genome cloning (New ...
... in an adverse manner. In this experiment,  pUC18 
Cloning and Transformation
Cloning and Transformation
Das, Surajit; Dash, Hirak Ranjan
and lambda DNA (λDNA) will be double digested ...
... Fidelity PCR system (Roche). 7. Cloning vector  pUC18 
Generating Knock-Out and Complementation Strains of Neisseria meningitidis
Generating Knock-Out and Complementation Strains of Neisseria meningitidis
Dam, Vincent; Bos, Martine P.
(New England Biolabs) or pCRII-TOPO ...
... pMB1 (ColE1) 4.4 ~20 Lin-Chao et al. (1992)  pUC18 
Plasmid Cloning Vectors
Plasmid Cloning Vectors
Julin, Douglas
pMB1 2.7 ~75 Lin-Chao et al. ...
... For example, if the BAC library was made in  pUC18 
Transposon-Mediated Sequencing
Transposon-Mediated Sequencing
Reeg, Rachel; Madan, Anup
( Fig. 2 ), cloned into the SmaI site, it would ...
... (OD660 ≤ 1) may allow increased protein yields.   
Novel Tools for the Functional Expression of Metagenomic DNA
Novel Tools for the Functional Expression of Metagenomic DNA
Katzke, Nadine; Knapp, Andreas; Loeschcke, Anita; Drepper, Thomas; Jaeger, Karl-Erich
...
... from Promega (Madison, WI). 6. Ready-to-go  pUC18 
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Manley, Darren; Neil, Joe D.
, pre-cut at the SmaI site, is from Pharmacia ...
... with 10X buffer (New England Biolabs). 20.  pUC18 
Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells
Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells
Gruel, Nadège; Kotlan, Beatrix; Beuzard, Marie; Teillaud, Jean-Luc
(SmaI/BAP) plasmid (Amersham Pharmacia ...