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... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... materials For binding experiments plasmid DNA (  pUC18 
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... containing a large spacer DNA sequence of  pUC18 
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
Wang, Baoli; Li, Xiaoxia; Zheng, Fang; Liu, Rui; Quan, Jinxing; Liang, Hui; Guo, Shanyi; Guo, Gang; Zhang, Jingyu
. ...
... isolated from kimchi This study Plasmid       pUC18 
Identification of a replicon from pCC3, a cryptic plasmid from Leuconostoc citreum C4 derived from kimchi, and development of a new host–vector system
Identification of a replicon from pCC3, a cryptic plasmid from Leuconostoc citreum C4 derived from kimchi, and development of a new host–vector system
Chang, Ji Yoon; Chang, Hae Choon
2,686 bp, E. coli cloning vector, Apr, lacZ ...
... 2 μ-based multicopy vector, LEU2 Clontech    pUC18 
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Konishi, Jin; Fukuda, Akira; Mutaguchi, Kozue; Uemura, Takeshi
Cloning vector Takara Bio  pAUR135 Cloning ...
... digested with BamHI and SacI and ligated into  pUC18 
Overexpression of AaPal, a peptidoglycan-associated lipoprotein from Alkalomonas amylolytica, improves salt and alkaline tolerance of Escherichia coli and Arabidopsis thaliana
Overexpression of AaPal, a peptidoglycan-associated lipoprotein from Alkalomonas amylolytica, improves salt and alkaline tolerance of Escherichia coli and Arabidopsis thaliana
Zhai, Lei; Xue, Yanfen; Song, Yuehao; Xian, Mingjie; Yin, Liang; Zhong, Naiqin; Xia, Guixian; Ma, Yanhe
at the two sites. ...
... in E. coli DH5α. Recombinant vectors  pUC18 
Construction of Escherichia coli strains producing l-serine from glucose
Construction of Escherichia coli strains producing l-serine from glucose
Li, Yu; Chen, Gu-Kui; Tong, Xin-Wei; Zhang, Hui-Tu; Liu, Xiao-Guang; Liu, Yi-Han; Lu, Fu-Ping
-serA and pTRC99a-serA were constructed from ...
... by Bryan et al. (2000) and provided as a gift,  pUC18 
Nisin-induced expression of a recombinant antihypertensive peptide in dairy lactic acid bacteria
Nisin-induced expression of a recombinant antihypertensive peptide in dairy lactic acid bacteria
Renye, John A., Jr.; Somkuti, George A.
was purchased from Life Technologies Inc. ...
... theEscherichia coli expression vector system,  pUC18 
cDNA cloning and expression of aTalaromyces emersonii amylase encoding genetic determinant inEscherichia coli
cDNA cloning and expression of aTalaromyces emersonii amylase encoding genetic determinant inEscherichia coli
Bunni, L.; Coleman, D. C.; McHale, L.; Hackett, T. J.; McHale, A. P.
and this was used to transformE. coli. ...
... hydrolyzed DNA into discrete fragments:  pUC18 
BpaI and BpnI: novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus
BpaI and BpnI: novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus
Jutur, Pavan P.; Reddy, Attipalli R.
(2.6 kb for BpaI; 1.8 and 0.8 kb for BpnI), ...
... xylinum plasmid pFF6 toEscherichia coli plasmid  pUC18 
Construction of shuttle vectors derived fromAcetobacter xylinum for cellulose-producing bacteriumAcetobacter xylinum
Construction of shuttle vectors derived fromAcetobacter xylinum for cellulose-producing bacteriumAcetobacter xylinum
Fujiwara, Masashi; Fukushi, Keiko; Takai, Mitsuo; Hayashi, Jisuke; Fukaya, Masahiro; Okumura, Hajime; Kawamura, Yoshiya
. ...
... sp. plasmid pIH41 intoEscherichia coli plasmid  pUC18 
A new shuttle vector forBacillus stearothermophilus andEscherichia coli
A new shuttle vector forBacillus stearothermophilus andEscherichia coli
Nakayama, Noriyuki; Narumi, Issay; Nakamoto, Shinya; Kihara, Hiroshi
. ...
... The enzyme dephosphorylated linearized  pUC18 
An Acid Phosphatase from Manihot glaziovii as an Alternative to Alkaline Phosphatase for Molecular Cloning Experiments
An Acid Phosphatase from Manihot glaziovii as an Alternative to Alkaline Phosphatase for Molecular Cloning Experiments
Tham, S.C.; Lim, S.H.; Yeoh, H.H.
DNA and preventing self-ligation under the same ...
... Hind III-XbaI, bdhA was ligated separately into  pUC18 
Metabolic engineering of Bacillus subtilis for enhanced production of acetoin
Metabolic engineering of Bacillus subtilis for enhanced production of acetoin
Wang, Meng; Fu, Jing; Zhang, Xueyu; Chen, Tao
to yield plasmid pUC18W01. ...
... coli NEB 5-alpha (New England Biolabs) and  pUC18 
Structural peculiarities of linear megaplasmid, pLMA1, from Micrococcus luteus interfere with pyrosequencing reads assembly
Structural peculiarities of linear megaplasmid, pLMA1, from Micrococcus luteus interfere with pyrosequencing reads assembly
Wagenknecht, Martin; Dib, Julián R.; Thürmer, Andrea; Daniel, Rolf; Farías, María E.; Meinhardt, Friedhelm
were used for cloning PstI restriction ...
... respectively. The multiple cloning sites of  pUC18 
Expression of a lipase on the cell-surface of Escherichia coli using the OmpW anchoring motif and its application to enantioselective reactions
Expression of a lipase on the cell-surface of Escherichia coli using the OmpW anchoring motif and its application to enantioselective reactions
Lee, Hyuk; Park, Si Jae; Han, Mee-Jung; Eom, Gyeong Tae; Choi, Min-Jung; Kim, Seong Ho; Oh, Young Hoon; Song, Bong Keun; Lee, Seung Hwan
(51 bp fragment) obtained by digestion of ...
... of Industrial Culture collection Plasmids  pUC18 
Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif
Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif
He, Ming-xiong; Feng, Hong; Zhang, Yi-zheng
bla cloning vector Takara pMD-18T T-A cloning ...
... using TA cloning that is constructed from the  pUC18 
Selecting DNA aptamers for endotoxin separation
Selecting DNA aptamers for endotoxin separation
Ying, GuoQing; Zhu, FangFang; Yi, Yu; Chen, JianShu; Mei, JianFeng; Zhang, YanLu; Chen, ShuQing
vector, and has the same functionality of ...
... Tn5 I and O ends, tnp, bla Purvis et al. 2005  pUC18 
Re-engineering Escherichia coli for ethanol production
Re-engineering Escherichia coli for ethanol production
Yomano, L. P.; York, S. W.; Zhou, S.; Shanmugam, K. T.; Ingram, L. O.
ColE1, bla New England Biolabs ldhA deletion ...
... The amplified fragment was inserted into a  pUC18 
Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein
Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein
Bae, JooYoun; Mie, Masayasu; Kobatake, Eiry
vector digested with SmaI. ...
... fusion between the GFP expression cassette and  pUC18 
Use of the pyrG gene as a food-grade selection marker in Monascus
Use of the pyrG gene as a food-grade selection marker in Monascus
Wang, Bo-hua; Xu, Yang; Li, Yan-ping
was isolated from pSGF957 (Kim et al. 2003a, ...
... were obtained in sequence cloned into a  pUC18 
Use of Pseudomonas mendocina, or recombinant Escherichia coli cells expressing toluene-4-monooxygenase, and a cell-free tyrosinase for the synthesis of 4-fluorocatechol from fluorobenzene
Use of Pseudomonas mendocina, or recombinant Escherichia coli cells expressing toluene-4-monooxygenase, and a cell-free tyrosinase for the synthesis of 4-fluorocatechol from fluorobenzene
Nolan, Louise C.; O’Connor, Kevin E.
vector as a gift from Professor Brian Fox ...
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