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... putative oriT RA3 (Figure 1B) was cloned into  pUC18 
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
Godziszewska, Jolanta; Kulińska, Anna; Jagura-Burdzy, Grażyna
[23], it facilitated plasmid mobilization by ...
... of the IPTG-inducible promoter, AAEC189(  pUC18 
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Dai, Jianjun; Wang, Shaohui; Guerlebeck, Doreen; Laturnus, Claudia; Guenther, Sebastian; Shi, Zhenyu; Lu, Chengping; Ewers, Christa
:aatA+P) expressing aatA under the control of ...
... was conducted as previously described [17, 27].  pUC18 
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Jeong, Kwang Cheol; Hung, Kai Foong; Baumler, David J; Byrd, Jeffrey J; Kaspar, Charles W
plasmid DNA (300 ng) was mixed with varying ...
... intergenic region (Fig. 1A) was cloned into  pUC18 
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Samsonova, Natalya N; Smirnov, Sergey V; Altman, Irina B; Ptitsyn, Leonid R
vector and sequenced. ...
... coli, such as those derived from pBR322 or  pUC18 
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
So, Lok Yan; Chen, Wen-yang; Lacap-Bugler, Donnabella C; Seemann, Myriam; Watt, Rory M
, cannot be stably-maintained in Z. ...
... showed no products with the gDNA of Z. mays and  pUC18 
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Weihmann, Fabian; Eisermann, Iris; Becher, Rayko; Krijger, Jorrit-Jan; Hübner, Konstantin; Deising, Holger B.; Wirsel, Stefan G. R.
(Additional file 1A). ...
... as previously described [2] with either:  pUC18 
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Lee, David J; Bingle, Lewis EH; Heurlier, Karin; Pallen, Mark J; Penn, Charles W; Busby, Stephen JW; Hobman, Jon L
[17], 2,700 bp (high copy number plasmid), ...
... +++ AES1/pYMZ81 ++ AES153/pYMZ81 + IHE3034/  pUC18 
Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains
Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains
Enow, Constance Oben Ayuk; Oscarsson, Jan; Zlatkov, Nikola; Westermark, Marie; Duperthuy, Marylise; Wai, Sun Nyunt; Uhlin, Bernt Eric
- AES1/ ...
... 16 16 8 4 ND DH5α E. coli 4 4 - - - - ND DH5α-  pUC18 
High prevalence of Cfr-producing Staphylococcus species in retail meat in Guangzhou, China
High prevalence of Cfr-producing Staphylococcus species in retail meat in Guangzhou, China
Zeng, Zhen-Ling; Wei, Hong-Kun; Wang, Jing; Lin, Da-Chuan; Liu, Xiao-Qin; Liu, Jian-Hua
-cfr E. coli 8 8 - - - - ND ATCC 29213 S. ...
... NIZO, Ede, The Netherlands. Plasmid  pUC18 
Re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of Listeria monocytogenes to β-lactam antibiotics
Re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of Listeria monocytogenes to β-lactam antibiotics
Krawczyk-Balska, Agata; Popowska, Magdalena; Markiewicz, Zdzislaw
[24] was obtained from the collection of the ...
... glnK gene this work pTZ19R ApR lacZ f1 IG [42]  pUC18 
Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1
Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1
Noindorf, Lilian; Bonatto, Ana C; Monteiro, Rose A; Souza, Emanuel M; Rigo, Liu U; Pedrosa, Fabio O; Steffens, Maria BR; Chubatsu, Leda S
ApR, lacZ, f1 Invitrogen pUCG08del 0.8 kb DNA ...
... excised from the gel, purified, and cloned into  pUC18 
Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
Bélanger, Louise; Charles, Trevor C
vector from which the insert DNA could be ...
... leu2-3-112 lys 1-1 trp1-289 ura3-52). Plasmids  pUC18 
Hepatitis B virus X protein impedes the DNA repair via its association with transcription factor, TFIIH
Hepatitis B virus X protein impedes the DNA repair via its association with transcription factor, TFIIH
Qadri, Ishtiaq; Fatima, Kaneez; AbdeL-Hafiz, Hany
and pBR322 were used for repair synthesis ...
... restricted with 10 U of Sau3A, and cloned into  pUC18 
Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid
Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid
Wiesner, Magdalena; Fernández-Mora, Marcos; Cevallos, Miguel A; Zavala-Alvarado, Crispín; Zaidi, Mussaret B; Calva, Edmundo; Silva, Claudia
digested with BamHI using standard methods ...
... genomic DNA using an internal bla fragment (  pUC18 
The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis
The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis
Mei, Xiaofen; Xu, Kai; Yang, Lingling; Yuan, Zhiming; Mahillon, Jacques; Hu, Xiaomin
), ISces and km as probes (Figure 4). ...
... Relevant characteristics Reference Purpose  pUC18 
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense
Sant'Anna, Fernando H; Andrade, Dieime S; Trentini, Débora B; Weber, Shana S; Schrank, Irene S
cloning vector, ampr, lacZα Clontech cloning ...
... 204 chromosomal DNA, subcloned into  pUC18 
Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system
Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system
Mahfouz, Magdy E; Grayson, T Hilton; Dance, David AB; Gilpin, Martyn L
, sequenced on both strands to confirm the ...
... Tyramine producer IPLA Collection PLASMIDS  pUC18 
The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH
The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH
Linares, Daniel M; Fernández, Maria; Del-Río, Beatriz; Ladero, Victor; Martin, Maria Cruz; Alvarez, Miguel A
AmpR [39] pNZ9530 EryR, nisR-nisK [40] pILORI4 ...
... bp EcoR I-Kpn I fragment A of tatA cloned into  pUC18 
Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae
Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae
Zhang, Lijuan; Zhu, Zhaoqin; Jing, Huaiqi; Zhang, Jingyun; Xiong, Yanwen; Yan, Meiying; Gao, Shouyi; Wu, Long-Fei; Xu, Jianguo; Kan, Biao
This study pT2 461 bp Xba I-Pst I fragment B of ...
... was cut with SmaI and cloned into the vector  pUC18 
Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
Heisig, Martin; Frentzen, Alexa; Bergmann, Birgit; Galmbacher, Katharina; Gentschev, Ivaylo; Hotz, Christian; Schoen, Christoph; Stritzker, Jochen; Fensterle, Joachim; Rapp, Ulf R; Goebel, Werner
, leading to the plasmid ...
... control cultures not exposed to Tat. Negative (  pUC18 
Proliferative activity of extracellular HIV-1 Tat protein in human epithelial cells: expression profile of pathogenetically relevant genes
Proliferative activity of extracellular HIV-1 Tat protein in human epithelial cells: expression profile of pathogenetically relevant genes
Bettaccini, Alessia A; Baj, Andreina; Accolla, Roberto S; Basolo, Fulvio; Toniolo, Antonio Q
) and positive controls (GAPDH, cyclophillin A, ...
... Easy Ampr lacZ, cloning vector Promega      pUC18 
Sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain Ochrobactrum tritici SCII24T
Sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain Ochrobactrum tritici SCII24T
Branco, Rita; Chung, Ana-Paula; Morais, Paula V
Ampr lacZ, cloning vector Invitrogen    pTRC ...
... resistance cassette was amplified from  pUC18 
2D proteome analysis initiates new Insights on the SalmonellaTyphimurium LuxS protein
2D proteome analysis initiates new Insights on the SalmonellaTyphimurium LuxS protein
Kint, Gwendoline; Sonck, Kathleen AJ; Schoofs, Geert; De Coster, David; Vanderleyden, Jos; De Keersmaecker, Sigrid CJ
-2 [51] using primers PRO-194 and PRO-195, ...
... gene, we replaced 2200 nt of the phx1 + ORF in  pUC18 
A homeobox protein Phx1 regulates long-term survival and meiotic sporulation in Schizosaccharomyces pombe
A homeobox protein Phx1 regulates long-term survival and meiotic sporulation in Schizosaccharomyces pombe
Kim, Ji-Yoon; Kwon, Eun-Soo; Roe, Jung-Hye
-phx1 + recombinant plasmid with a ura4+ ...
... leuB6 thi-1) Laboratory collection Plasmids  pUC18 
The YvfTU Two-component System is involved in plcR expression in Bacillus cereus
The YvfTU Two-component System is involved in plcR expression in Bacillus cereus
Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolstø, Anne-Brit; Nguyen-the, Christophe; Lereclus, Didier; Broussolle, Véronique
ApR cloning vector Laboratory collection pMAD ...

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