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... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... expression Datsenko and Wanner (2000)  pUC18 
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
Yang, Ying; Yang, Yi; Ou, Bingming; Xia, Pengpeng; Zhou, Mingxu; Li, Luan; Zhu, Guoqiang
Ampr, cloning vector Lab stock ...
... relA1 Clontech, Heidelberg, Germany Plasmids  pUC18 
The response of Serratia marcescens JG to environmental changes by quorum sensing system
The response of Serratia marcescens JG to environmental changes by quorum sensing system
Sun, Shu-Jing; Liu, Hui-Jun; Weng, Cai-Hong; Lai, Chun-Fen; Ai, Liu-Ying; Liu, Yu-Chen; Zhu, Hu
pBR322-derived cloning vector, Lac+, Apr MBI ...
... with angD, constructed in  pET43.1a This work    pUC18 
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Liu, Qin; Ma, Yue; Zhou, Lingyun; Zhang, Yuanxing
Cloning/expression vector Sangon (Shanghai, ...
... Relevant characteristics Reference Plasmids    pUC18 
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
Ding, Nianhua; Zheng, Ying; Wu, Qian; Mao, Xiaohua
Cloning vector, ApR Yanisch-Perron et al. ...
... of a recombinant plasmid pFUC (a derivative of  pUC18 
Preliminary characterization of a thermostable DNA polymerase I from a mesophilic Bacillus sphaericus strain C3-41
Preliminary characterization of a thermostable DNA polymerase I from a mesophilic Bacillus sphaericus strain C3-41
Bei, Han; Haizhou, Liu; Xiaomin, Hu; Zhiming, Yuan
with a DNA insert) was used as DNA template and ...
... present study Plasmid Relevant genotype Source  pUC18 
N terminus determinants of MinC from Neisseria gonorrhoeae mediate interaction with FtsZ but do not affect interaction with MinD or homodimerization
N terminus determinants of MinC from Neisseria gonorrhoeae mediate interaction with FtsZ but do not affect interaction with MinD or homodimerization
Greco-Stewart, V.; Ramirez-Arcos, S.; Liao, M.; Dillon, J. R.
AmpRPlac Amersham Pharmacia pSR2 AmpRPlac::minC ...
... in acetate minimal medium This work Plasmids    pUC18 
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis
Netzer, Roman; Krause, Malgorzata; Rittmann, Doris; Peters-Wendisch, Petra G.; Eggeling, Lothar; Wendisch, Volker F.; Sahm, Hermann
Cloning vector; Apr Norrander et al. (1983) ...
... recA∷Rp-2 Tc∷Mu Simon et al. (1983) Plasmids    pUC18 
Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs
Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs
Brichta, Dayna M.; Azad, Kamran N.; Ralli, Pooja; O’Donovan, Gerard A.
/19 Apr cloning vectors, source of lacZα ...
... chromosome, Smr Simon et al. (1983) Plasmids    PUC18 
Poly β-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant
Poly β-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant
Kadouri, Daniel; Jurkevitch, Edouard; Okon, Yaacov
Apr, ColE1 replicon, lacZ, cloning vector ...
... protein Omp31 was amplified, cloned into  pUC18 
Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells
Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells
Verdiguel-Fernández, L.; Oropeza-Navarro, R.; Basurto-Alcántara, Francisco J.; Castañeda-Ramírez, A.; Verdugo-Rodríguez, Antonio
plasmid, and inactivated by inserting a ...
... σ70 promoter M. Surette Kim and Surette 2006  pUC18 
The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
Herrington, Muriel B.; Sitaras, Chris
AmpR, colE1 ori C. ...
... used as host of the cloning experiments, and  pUC18 
Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate
Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate
Wei, Ping; Bai, Liping; Song, Wengang; Hao, Gangping
was employed as vector. ...
... gene cloned on an SphI fragment This work    pUC18 
Distinct mechanisms regulate expression of the two major groEL homologues in Rhizobium leguminosarum
Distinct mechanisms regulate expression of the two major groEL homologues in Rhizobium leguminosarum
Gould, Phillip; Maguire, Maria; Lund, Peter A.
ApR; cloning vector Yanisch-Perron et al. ...
... replicon; cos Mob+, Tcr Kimbara et al. (1989)    pUC18 
Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205
Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205
Ito, Michihiro; Prokop, Zbyňek; Klvaňa, Martin; Otsubo, Yoshiyuki; Tsuda, Masataka; Damborský, Jiří; Nagata, Yuji
pMB9 replicon; Apr Vieira and Messing (1982) ...
... sp. FLA in BglII site of pIJ702 This study  pUC18 
A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols
A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols
Merkens, Hedda; Sielker, Sonja; Rose, Karsten; Fetzner, Susanne
ColE1 lacZ, bla Vieira and Messing (1982) ...
... [aac(3)IV] Blondelet-Rouault et al. (1997)    pUC18 
Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans
Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans
Guyet, Aurélie; Gominet, Myriam; Benaroudj, Nadia; Mazodier, Philippe
Cloning vector in E. coli Yanisch-Perron et al. ...
... Expression host, Camr (Chloramphenicol) Novagen  pUC18 
Genetic and biochemical analyses of chlorobenzene degradation gene clusters in Pandoraea sp. strain MCB032
Genetic and biochemical analyses of chlorobenzene degradation gene clusters in Pandoraea sp. strain MCB032
Jiang, Xi-Wen; Liu, Hong; Xu, Ying; Wang, Shu-Jun; Leak, David J.; Zhou, Ning-Yi
Cloning vector, Ampr (Ampicillin) Takara pET5a ...
... was ligated into the XabI and HindIII sites of  pUC18 
Excision of the nifD element in the heterocystous cyanobacteria
Excision of the nifD element in the heterocystous cyanobacteria
Henson, B. J.; Pennington, L. E.; Watson, L. E.; Barnum, S. R.
, creating pRE-18. pDK1 and pDK3 The plasmid ...
... Complete angE cloned in pMD18 This work    pUC18 
Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1
Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1
Liu, Qin; Ma, Yue; Wu, Haizhen; Shao, Mingfei; Liu, Haifeng; Zhang, Yuanxing
Cloning/expression vector Sangon (Shanghai, ...
... cloned into the multiple cloning sites of the  pUC18 
ISCR2 is associated with the dissemination of multiple resistance genes among Vibrio spp. and Pseudoalteromonas spp. isolated from farmed fish
ISCR2 is associated with the dissemination of multiple resistance genes among Vibrio spp. and Pseudoalteromonas spp. isolated from farmed fish
Xu, Yixiang; Wang, Chao; Zhang, Gang; Tian, Jingjing; Liu, Ying; Shen, Xihui; Feng, Jie
vector. ...
... and its flanking sequences were cloned into  pUC18 
Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenases
Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenases
Synstad, Bjørnar; Emmerhoff, Oddmund; Sirevåg, R.
/19, indicating that the putative σ70 promoter ...
... pGEM3zF+lacZ′-dnaK Vh AmpR This work  pMZ40  pUC18 
Complementation studies of the DnaK–DnaJ–GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro
Complementation studies of the DnaK–DnaJ–GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro
Żmijewski, Michał A.; Kwiatkowska, Joanna M.; Lipińska, Barbara
-grpE Vh AmpR This work  pBR322 AmpR, TetR Our ...
... kgmB gene. The kgmB gene was first cloned into  pUC18 
The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated
The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated
Vajic, Sandra; Anastasov, Natasa; Vasiljevic, Branka
to yield pUΔK1. ...
... + sacB +, gene replacement vector with MCS from  pUC18 
The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa
The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa
Gliese, Nicole; Khodaverdi, Viola; Görisch, Helmut
Hoang et al. (1998)   pLAFR3 Tcr; ...

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