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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... from Novagen (Darmstadt, Germany). The plasmid  pUC18 
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Liu, D.; Schmid, R. D.; Rusnak, M.
was purchased from MBI Fermentas (St. ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... the lacZ-tagged strain B. subtilis Tu-100(  pUC18 
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Hu, Xiaojia; Roberts, Daniel P.; Jiang, Mulan; Zhang, Yinbo
) was detected within all roots of oilseed ...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... feces (serotype O1483: H1) ATCC Plasmids    pUC18 
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Zhou, Lingyun; Liu, Qin; Wang, Qiyao; Ma, Yue; Xu, Yuzhou; Yang, Zhao; Zhao, Yan; Zhang, Yuanxing
Cloning/expression vector, Apr lacZ-α, Ori: ...
... of the 3′-end region of the GPD1. The plasmid  pUC18 
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Kong, Qing-Xue; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun
-R was created by inserting a 446-bp of ...
... the construction of the integration vector were  pUC18 
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Zhang, Huitu; Tian, Yao; Wang, Jianling; Li, Yonghai; Wang, Haikuan; Mao, Shuhong; Liu, Xiaoguang; Wang, Chunxia; Bie, Songtao; Lu, Fuping
and pUC57. ...
... along with chrysotile asbestos fibers and  pUC18 
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Yoshida, N.; Saeki, Y.
donor DNA, was spread over the surface of a ...
... of STE2 encoding alpha-factor receptor, plasmid  pUC18 
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Liu, Jing-Jing; Ding, Wen-Tao; Zhang, Guo-Chang; Wang, Jing-Yu
-ste2-URA was constructed. ...
... cosmid was further constructed into a  pUC18 
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Wang, Jianjun; Zhu, Yaxin; Zhao, Guogang; Zhu, Junge; Wu, Sheng
plasmid library and screened by the same ...
... Simon et al., 1983 pT7Blue(R) Apr, lacZ Novagen  pUC18 
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Shintani, Masaki; Yoshida, Takako; Habe, Hiroshi; Omori, Toshio; Nojiri, Hideaki
/19 Apr, lacZ, pMB9 replicon Sambrook and ...
... RNAP was established. The template plasmid is a  pUC18 
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium
Stammen, Simon; Schuller, Franziska; Dietrich, Sylvia; Gamer, Martin; Biedendieck, Rebekka; Jahn, Dieter
derivative, which enables blue/white screening ...
... Plasmid Characteristics Reference and source  pUC18 
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Kasuga, Kano; Nitta, Atsushi; Kobayashi, Masayuki; Habe, Hiroshi; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio; Kojima, Ikuo
/19 bla, lacZ′, pMB9 replicon Yanisch-Perron et ...
... and screened two subclones in E. coli. One is  pUC18 
Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli
Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli
Fu, Shulin; Zhang, Weicai; Guo, Aiguang; Wang, Jianhua
-SDH, which contained sdh; another is ...
... Genomic insertion of eGFP This study Plasmids    pUC18 
A three-step method for analysing bacterial biofilm formation under continuous medium flow
A three-step method for analysing bacterial biofilm formation under continuous medium flow
Schmutzler, Karolin; Schmid, Andreas; Buehler, Katja
_mini-Tn7-Gm-T7 ...
... Genotype or descriptiona Source or reference  pUC18 
Canthaxanthin production with modified Mucor circinelloides strains
Canthaxanthin production with modified Mucor circinelloides strains
Papp, Tamás; Csernetics, Árpád; Nagy, Gábor; Bencsik, Ottó; Iturriaga, Enrique A.; Eslava, Arturo P.; Vágvölgyi, Csaba
General cloning vector for E. coli (Amp) ...
... to erythromycin (Gryczan et al. 1980) Plasmids  pUC18 
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
Wu, Hang; Li, Weiwei; Xin, Chen; Zhang, Congming; Wang, Yansheng; Ren, Shaohua; Ren, Min; Zhao, Wei; Yuan, Li; Xu, Zhongdong; Yuan, Hualing; Geng, Ming; Zhang, Lixin; Weaver, David T.; Zhang, Buchang
General cloning vector (Sambrook and Russell ...
... as control for transformation efficiency. The  pUC18 
Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells
Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells
Aachmann, Finn Lillelund; Aune, Trond Erik Vee
plasmid with ampicillin resistance marker ...
... The C427-encoding gene was subcloned into a  pUC18 
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Nagao, Koji; Yamashita, Michio; Ueda, Mitsuyoshi
vector. pYS9107K, a plasmid for C427 expression ...
... then inserted into the corresponding sites of  pUC18 
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Choi, Seon-Kang; Harada, Hisashi; Matsuda, Satoru; Misawa, Norihiko
vector (Toyobo) to, respectively, construct ...
... or description Source E. coli originated  pUC18 
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Matsui, Toru; Saeki, Hisashi; Shinzato, Naoya; Matsuda, Hitoshi
Cloning vector, Apr Takara R. rhodochrous ...
... in stationary phase, chrysotile asbestos, and  pUC18 
Escherichia coli cells penetrated by chrysotile fibers are transformed to antibiotic resistance by incorporation of exogenous plasmid DNA
Escherichia coli cells penetrated by chrysotile fibers are transformed to antibiotic resistance by incorporation of exogenous plasmid DNA
Yoshida, N.; Kodama, K.; Nakata, K.; Yamashita, M.; Miwa, T.
donor DNA spread over the surface of a ...

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