Snippet Search

Searching 11,460,015 documents.
Discover how a particular term or phrase is used in scientific literature

Showing 1 to 25 of 328 matching articles

Results per page:

... μl) was performed with negatively supercoiled  pUC18 
A functional type I topoisomerase from Pseudomonas aeruginosa
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...
... methods Reagents and biochemicals The plasmid  pUC18 
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Perry, Christopher C.; Urata, Sarah M.; Lee, Melissa; Aguilera, Joe A.; Milligan, Jamie R.
was grown on a liter scale in an E. coli host ...
... was conducted as previously described [17, 27].  pUC18 
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Jeong, Kwang Cheol; Hung, Kai Foong; Baumler, David J; Byrd, Jeffrey J; Kaspar, Charles W
plasmid DNA (300 ng) was mixed with varying ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... − lacZ − was cotransformed with various PutA-  pUC18 
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.
constructs (PutA- ...
... the lacZ-tagged strain B. subtilis Tu-100(  pUC18 
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Hu, Xiaojia; Roberts, Daniel P.; Jiang, Mulan; Zhang, Yinbo
) was detected within all roots of oilseed ...
... pSVJ21, was constructed by ligating p54 and  pUC18 
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Miteva, Vanya; Lantz, Sarah; Brenchley, Jean
and inserting a chloramphenicol acetyl ...
... the gene encoded in the plasmid substrates (  pUC18 
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan
: TS) for use in the cleavage reactions. (c) ...
... fragments of 1,769 and 676 bp from  pUC18 
Genome-specific primer sets for starch biosynthesis genes in wheat
Genome-specific primer sets for starch biosynthesis genes in wheat
Blake, N. K.; Sherman, J. D.; Dvořák, J.; Talbert, L. E.
digested with RsaI. ...
... sterile and fertile lines were cloned into the  pUC18 
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
Das, Srirupa; Sen, Supriya; Chakraborty, Anirban; Chakraborti, Papia; Maiti, Mrinal K; Basu, Asitava; Basu, Debabrata; Sen, Soumitra K
vector. ...
... the heme proteins converted the supercoiled  pUC18 
Endonuclease-like activity of heme proteins
Endonuclease-like activity of heme proteins
Tan, Willy B.; Cheng, Wunhuey; Webber, Andrew; Bhambhani, Akhilesh; Duff, Michael R.; Kumar, Challa V.; McLendon, George L.
DNA into nicked circular and linear DNA. ...
... and Chemicals pBluescript II KS (–),  pUC18 
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong
, and pIJ2925 were used for routine cloning and ...
... much noisier. To achieve this, we sequenced a  pUC18 
Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study
Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study
Goto, Hiroki; Dickins, Benjamin; Afgan, Enis; Paul, Ian M; Taylor, James; Makova, Kateryna D; Nekrutenko, Anton
plasmid isolated from a single colony, which in ...
... partially purified enzyme preparations using  pUC18 
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Gupta, Richa; Xu, Shuang-Yong; Sharma, Prince; Capalash, Neena
and/or λ DNA as substrates and Universal Buffer ...
... cosmid was further constructed into a  pUC18 
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Wang, Jianjun; Zhu, Yaxin; Zhao, Guogang; Zhu, Junge; Wu, Sheng
plasmid library and screened by the same ...
... below). Controls Nick-translated biotin-labeled  pUC18 
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Evans, Mark F; Aliesky, Holly A; Cooper, Kumarasen
plasmid was used as a control for non-specific ...
... In contrast, the high copy number plasmid  pUC18 
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Zhang, Junjie; De Masi, Leon; John, Beena; Chen, Wenxin; Schifferli, Dieter M
-OVA that constitutively expressed the ...
... and was subsequently used to generate the  pUC18 
Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek’s disease virus (MDV) alters expression of nearby MDV genes
Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek’s disease virus (MDV) alters expression of nearby MDV genes
Kim, Taejoong; Mays, Jody; Fadly, Aly; Silva, Robert F.
–RM1–LTR–kan transfer vector, using published ...
... further cloning of the synthetic genes into  pUC18 
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Lamport, Derek T. A.; Tan, Li; Kieliszewski, Marcia J.
-SS tob -EGFP vector. ...
... La Jolla, CA); pCMV-Sport6-Empty and  pUC18 
A functional map of NFκB signaling identifies novel modulators and multiple system controls
A functional map of NFκB signaling identifies novel modulators and multiple system controls
Halsey, Thomas A; Yang, Longlong; Walker, John R; Hogenesch, John B; Thomas, Russell S
-Empty (Invitrogen); pCS2-DsRed (kindly ...
... human SP-C gene promoter (3.7 kb) cloned into  pUC18 
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Cai, Yan; Yoneda, Mitsuhiro; Tomita, Takeshi; Kurotani, Reiko; Okamoto, Minoru; Kido, Taketomo; Abe, Hiroyuki; Mitzner, Wayne; Guha, Arjun; Kimura, Shioko
vector with SV40 small T intron and poly A ...
... pMB1 (ColE1) 4.4 ~20 Lin-Chao et al. (1992)  pUC18 
Plasmid Cloning Vectors
Plasmid Cloning Vectors
Julin, Douglas
pMB1 2.7 ~75 Lin-Chao et al. ...
... segments were synthesized and cloned in  pUC18 
Expression of an engineered synthetic cry2Aa (D42/K63F/K64P) gene of Bacillus thuringiensis in marker free transgenic tobacco facilitated full-protection from cotton leaf worm (S. littoralis) at very low concentration
Expression of an engineered synthetic cry2Aa (D42/K63F/K64P) gene of Bacillus thuringiensis in marker free transgenic tobacco facilitated full-protection from cotton leaf worm (S. littoralis) at very low concentration
Gayen, Srimonta; Mandal, Chandi Charan; Samanta, Milan Kumar; Dey, Avishek; Sen, Soumitra Kumar
vector according to Sambrook et al. (1989), and ...