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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... DNA from plasmid  pUC18 
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Shiina, Takuya; Watanabe, Ritsuko; Shiraishi, Iyo; Suzuki, Masao; Sugaya, Yuki; Fujii, Kentaro; Yokoya, Akinari
was irradiated with low-LET (13 keV/μm) or ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... pDONR™ P4-P1R, and pDONR™ P2R-P3 (see Note 1),  pUC18 
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Sone, Takefumi; Imamoto, Fumio
(8), template DNA for amplification of ...
... (1991)  CaMV35S-sGFP (S65T)-NOS3′ GFP in  pUC18 
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Kiyono, Masako; Oka, Yumiko; Sone, Yuka; Tanaka, Michitaka; Nakamura, Ryosuke; Sato, Masa H.; Pan-Hou, Hidemitsu; Sakabe, Kou; Inoue, Ken-ichiro
Uemura et al. (2004)  SYP111/KOLLE GFP-SYP111 ...
... Simon et al., 1983 pT7Blue(R) Apr, lacZ Novagen  pUC18 
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Shintani, Masaki; Yoshida, Takako; Habe, Hiroshi; Omori, Toshio; Nojiri, Hideaki
/19 Apr, lacZ, pMB9 replicon Sambrook and ...
... Plasmid Characteristics Reference and source  pUC18 
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Kasuga, Kano; Nitta, Atsushi; Kobayashi, Masayuki; Habe, Hiroshi; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio; Kojima, Ikuo
/19 bla, lacZ′, pMB9 replicon Yanisch-Perron et ...
... fragment was subcloned into the HindIII site of  pUC18 
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Noda, Shuhei; Miyazaki, Takaya; Miyoshi, Takanori; Miyake, Michiru; Okai, Naoko; Tanaka, Tsutomu; Ogino, Chiaki; Kondo, Akihiko
. ...
... the gene cloning experiments. Cloning vectors  pUC18 
Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans
Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans
Hirano, Shin-ichi; Haruki, Mitsuru; Takano, Kazufumi; Imanaka, Tadayuki; Morikawa, Masaaki; Kanaya, Shigenori
, pT7Blue-T, and pSTV28 were purchased from ...
... along with chrysotile asbestos fibers and  pUC18 
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Yoshida, N.; Saeki, Y.
donor DNA, was spread over the surface of a ...
... different plasmids were used in this study: the  pUC18 
Obtaining transgenic plants using the bio-active beads method
Obtaining transgenic plants using the bio-active beads method
Liu, Haibo; Kawabe, Akira; Matsunaga, Sachihiro; Murakawa, Tomoko; Mizukami, Atsushi; Yanagisawa, Masanobu; Nagamori, Eiji; Harashima, Satoshi; Kobayashi, Akio; Fukui, Kiichi
-sGFP (4.1-kb) plasmid containing a cauliflower ...
... We used Escherichia coli JM109 harboring  pUC18 
The use of nano-sized acicular material, sliding friction, and antisense DNA oligonucleotides to silence bacterial genes
The use of nano-sized acicular material, sliding friction, and antisense DNA oligonucleotides to silence bacterial genes
Mitsudome, Yuya; Takahama, Mamiko; Hirose, Jun; Yoshida, Naoto
DNA and Pseudomonas pseudoalcaligenes KF707 to ...
... terminal repeat, and this vector was named  pUC18 
A novel fusion protein that functions as an enhanced green fluorescent protein reporter and a tetracycline-controlled transcriptional activator
A novel fusion protein that functions as an enhanced green fluorescent protein reporter and a tetracycline-controlled transcriptional activator
Hara, Kimiko; Kuwayama, Hisashi; Inukai, Yoshiaki; Yaginuma, Toshinobu; Niimi, Teruyuki
-piggyBacR. ...
... The C427-encoding gene was subcloned into a  pUC18 
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Nagao, Koji; Yamashita, Michio; Ueda, Mitsuyoshi
vector. pYS9107K, a plasmid for C427 expression ...
... in Populus sp. plants, was cloned into  pUC18 
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Murata, Hitoshi; Sunagawa, Masahide; Yamazaki, Takashi; Shishido, Kazuo; Igasaki, Tomohiro
, generating pHHM181. dsred2 was newly ...
... or description Source E. coli originated  pUC18 
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Matsui, Toru; Saeki, Hisashi; Shinzato, Naoya; Matsuda, Hitoshi
Cloning vector, Apr Takara R. rhodochrous ...
... human SP-C gene promoter (3.7 kb) cloned into  pUC18 
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Cai, Yan; Yoneda, Mitsuhiro; Tomita, Takeshi; Kurotani, Reiko; Okamoto, Minoru; Kido, Taketomo; Abe, Hiroyuki; Mitzner, Wayne; Guha, Arjun; Kimura, Shioko
vector with SV40 small T intron and poly A ...
... Yeast Co. Ltd. (Tokyo, Japan). The plasmid  pUC18 
Gene cloning and biochemical characterization of 4-N-trimethylaminobutyraldehyde dehydrogenase II from Pseudomonas sp. 13CM
Gene cloning and biochemical characterization of 4-N-trimethylaminobutyraldehyde dehydrogenase II from Pseudomonas sp. 13CM
Bari, Md. Rezaul; Hassan, Maizom; Akai, Naoki; Arima, Jiro; Mori, Nobuhiro
(Takara Bio Inc., Japan) was used for shotgun ...
... pBeloBAC11 Cmr, BAC cloning vector Invitrogen  pUC18 
Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars
Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars
Genka, Hiroyuki; Baba, Tomoya; Tsuda, Masataka; Kanaya, Shigehiko; Mori, Hirotada; Yoshida, Takanobu; Noguchi, Masako Tsujimoto; Tsuchiya, Kenichi; Sawada, Hiroyuki
Apr, cloning vector TaKaRa pUC4K Apr Kmr, ...
... along with chrysotile asbestos fibers and  pUC18 
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Yoshida, Naoto; Takebe, Kazuki
donor DNA, was spread over the surface of an LB ...
... SalI, and the digested fragment was ligated to  pUC18 
Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana
Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana
Chung, Ho Yong; Fujioka, Shozo; Choe, Sunghwa; Lee, Soyoun; Lee, Youn Hyung; Baek, Nam In; Chung, In Sik
, creating ...
... bla erm Pspac-purE’ lacZ lacI This study  pUC18 
Accumulation of gene-targeted Bacillus subtilis mutations that enhance fermentative inosine production
Accumulation of gene-targeted Bacillus subtilis mutations that enhance fermentative inosine production
Asahara, Takayuki; Mori, Yukiko; Zakataeva, Natalia P.; Livshits, Vitaliy A.; Yoshida, Ken-ichi; Matsuno, Kiyoshi
bla Yanisch-Perron et al. 1985 pKM240 bla deoD ...
... then inserted into the corresponding sites of  pUC18 
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Choi, Seon-Kang; Harada, Hisashi; Matsuda, Satoru; Misawa, Norihiko
vector (Toyobo) to, respectively, construct ...
... chamber. Plasmid and mRNA 1. Plasmid:  pUC18 
Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
Kobayashi, Tominari; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz [2] (see Note 3). 2. A commercially ...