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... in Escherichia coli JM109 from an inducible  pUC18 
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Huang, Yanna; You, Chunping; Liu, Zhenmin
vector, respectively, and the resulting strains ...
... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... erm This study Plasmids    pUC18 
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Fu, Jing; Huo, Guangxin; Feng, Lili; Mao, Yufeng; Wang, Zhiwen; Ma, Hongwu; Chen, Tao; Zhao, Xueming
Ampr Lab Stock   ...
... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... for AFM For AFM imaging in air, 100.0 μg/ml  pUC18 
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
Shen, Xin-Cheng; Bao, Lei; Zhang, Zhi-Ling; Liu, Xiaoshen; Pang, Dai-Wen; Xu, Jianrong
DNA sample was diluted into final concentration ...
... of the IPTG-inducible promoter, AAEC189(  pUC18 
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Dai, Jianjun; Wang, Shaohui; Guerlebeck, Doreen; Laturnus, Claudia; Guenther, Sebastian; Shi, Zhenyu; Lu, Chengping; Ewers, Christa
:aatA+P) expressing aatA under the control of ...
... E. coli cloning vector, 4.3 kb TaKaRa     pUC18 
Role of DNA in Bacterial Aggregation
Role of DNA in Bacterial Aggregation
Liu, Hui-Hui; Yang, Yi-Ran; Shen, Xin-Cheng; Zhang, Zhi-Ling; Shen, Ping; Xie, Zhi-Xiong
E. coli cloning vector, 2.7 kb TaKaRa   Herring ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... with two XcmI cassettes was inserted into  pUC18 
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
Gu, Jingsong; Ye, Chunjiang
to construct an efficient XcmI T-vector parent ...
... expressions of DR4 and DR5, we transfected the  pUC18 
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
Kong, Fanyun; You, Hongjuan; Zhao, Jinjin; Liu, Wen; Hu, Lei; Luo, Wenya; Hu, Wei; Tang, Renxian; Zheng, Kuiyang
-HBV1.2, a plasmid contains the full-length HBV ...
... (carrying wildtype dnaQ), and the empty vector  pUC18 
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Luan, Guodong; Cai, Zhen; Li, Yin; Ma, Yanhe
, was transformed into E. coli cells, ...
... and Chemicals pBluescript II KS (–),  pUC18 
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong
, and pIJ2925 were used for routine cloning and ...
... respectively, and subcloned into the plasmid  pUC18 
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Yang, Xue-Peng; Zhong, Gui-Fang; Lin, Jin-Ping; Mao, Duo-Bin; Wei, Dong-Zhi
between the EcoRI–PstI sites downstream of the ...
...  pPM Cmr, Emr; pHP13 (P43b ::mdh) This study    pUC18 
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Mu, Li; Wen, Jianping
E. coli cloning vector; Ampr Laboratory stock ...
... 621H pGOX3, and E. coli cloning vector  pUC18 
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Zhang, Lin; Lin, Jinping; Ma, Yushu; Wei, Dongzhi; Sun, Ming
. ...
... with the appropriate antibiotic. The pYES2,  pUC18 
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Huang, Xiao-mei; Li, Qing-qing; Chen, Xiu-ling; Fan, Jin-xia; Xu, Xiu-hong; Sun, Xu-dong; Li, Dong-yu; Zhao, Hong-xiao
, pPIC9K, and pCSN43 plasmids were used for the ...
... for promoter screening CGMCC 3584 Plasmids    pUC18 
Screening of promoters from Arthrobacter sp. CGMCC 3584 using a green fluorescent protein reporter system
Screening of promoters from Arthrobacter sp. CGMCC 3584 using a green fluorescent protein reporter system
Niu, Huanqing; Yang, Wei; Zhuang, Kun; Chen, Xiaochun; Chen, Yong; Liu, Dong; Wu, Jinglan; Zhu, Chenjie; Ying, Hanjie
Ampr, 2.686 kb Clontech  Plac− ...
... Relevant characteristics Reference Plasmids    pUC18 
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
Ding, Nianhua; Zheng, Ying; Wu, Qian; Mao, Xiaohua
Cloning vector, ApR Yanisch-Perron et al. ...
... to erythromycin (Gryczan et al. 1980) Plasmids  pUC18 
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
Wu, Hang; Li, Weiwei; Xin, Chen; Zhang, Congming; Wang, Yansheng; Ren, Shaohua; Ren, Min; Zhao, Wei; Yuan, Li; Xu, Zhongdong; Yuan, Hualing; Geng, Ming; Zhang, Lixin; Weaver, David T.; Zhang, Buchang
General cloning vector (Sambrook and Russell ...
... from Sigma Co., Ltd. (Shanghai, China).  pUC18 
The Synthesis of l-dopa-l-Tyr and The Interaction of l-dopa-l-Tyr With ctDNA
The Synthesis of l-dopa-l-Tyr and The Interaction of l-dopa-l-Tyr With ctDNA
Lu, Kui; Sun, Jing; Zhao, Dong-Xin; Zhu, Qi-Cong; Lv, Wei
DNA was provided by Fermentas Co., Ltd. ...
... of the trc promoter This work Plasmids    pUC18 
Succinic Acid Production from Corn Cob Hydrolysates by Genetically Engineered Corynebacterium glutamicum
Succinic Acid Production from Corn Cob Hydrolysates by Genetically Engineered Corynebacterium glutamicum
Wang, Chen; Zhang, Hengli; Cai, Heng; Zhou, Zhihui; Chen, Yilu; Chen, Yali; Ouyang, Pingkai
Apr; a-lac/MCS M13 ori Takara   ...
... in this study Store in our lab [21] Plasmids    pUC18 
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Liao, Yan; Li, Chun-mei; Chen, Hui; Wu, Qi; Shan, Zhi; Han, Xue-yi
-phyA m Modified phyA fragment cloned into the ...
... with angD, constructed in  pET43.1a This work    pUC18 
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Liu, Qin; Ma, Yue; Zhou, Lingyun; Zhang, Yuanxing
Cloning/expression vector Sangon (Shanghai, ...