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... in Escherichia coli JM109 from an inducible  pUC18 
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Cloning of d-lactate dehydrogenase genes of Lactobacillus delbrueckii subsp. bulgaricus and their roles in d-lactic acid production
Huang, Yanna; You, Chunping; Liu, Zhenmin
vector, respectively, and the resulting strains ...
... nucleases were studied using supercoiled  pUC18 
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Hangan, Adriana Corina; Stan, Roxana Liana; Turza, Alexandru; Oprean, Luminiţa Simona; Páll, Emöke; Gheorghe-Cetean, Sînziana; Sevastre, Bogdan
DNA in cacodylate buffer (0.1 M, pH 6.0), in ...
... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... from the pDONR 221 plasmid, cloned into the  pUC18 
pELMO, an optimised in-house cloning vector
pELMO, an optimised in-house cloning vector
Ramos, Andrea E.; Muñoz, Marina; Moreno-Pérez, Darwin A.; Patarroyo, Manuel A.
vector’s multiple cloning site (Thermo ...
... with the appropriate antibiotic. The pYES2,  pUC18 
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Huang, Xiao-mei; Li, Qing-qing; Chen, Xiu-ling; Fan, Jin-xia; Xu, Xiu-hong; Sun, Xu-dong; Li, Dong-yu; Zhao, Hong-xiao
, pPIC9K, and pCSN43 plasmids were used for the ...
... pIJ101 aac(3)IV ermEp; oriT RK2 [16] pL98 7.4  pUC18 
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Rebets, Yuriy; Kormanec, Jan; Luzhetskyy, Andriy; Bernaerts, Kristel; Anné, Jozef
aac(3)IV pIJ101 aac(3)IV ssrAp; oriT RK2 [16] ...
... arms of hyaluronidase gene was inserted into  pUC18 
Improved Yield of High Molecular Weight Hyaluronic Acid Production in a Stable Strain of Streptococcus zooepidemicus via the Elimination of the Hyaluronidase-Encoding Gene
Improved Yield of High Molecular Weight Hyaluronic Acid Production in a Stable Strain of Streptococcus zooepidemicus via the Elimination of the Hyaluronidase-Encoding Gene
Pourzardosht, Navid; Rasaee, Mohammad Javad
plasmid to construct ...
... The resulting BamHI fragments were cloned into  pUC18 
Gene Overexpression in Streptomyces hygroscopicus Associated with DNA Amplification
Gene Overexpression in Streptomyces hygroscopicus Associated with DNA Amplification
Cao, Guangxiang; Zhang, Peipei; Gu, Yuanxin; Pang, Xiuhua
pretreated with BamHI, and the E. coli ...
... protection of fully hydroxymethylated 5hmC  pUC18 
Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA
Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA
Skvortsova, Ksenia; Zotenko, Elena; Luu, Phuc-Loi; Gould, Cathryn M.; Nair, Shalima S.; Clark, Susan J.; Stirzaker, Clare
was ~100% (“Methods” section; Additional file ...
... (OD660 ≤ 1) may allow increased protein yields.   
Novel Tools for the Functional Expression of Metagenomic DNA
Novel Tools for the Functional Expression of Metagenomic DNA
Katzke, Nadine; Knapp, Andreas; Loeschcke, Anita; Drepper, Thomas; Jaeger, Karl-Erich
...
... by introducing it into an expression vector  pUC18 
Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester
Protein Engineering and Homologous Expression of Serratia marcescens Lipase for Efficient Synthesis of a Pharmaceutically Relevant Chiral Epoxyester
Chen, Ke-Cai; Zheng, Ming-Min; Pan, Jiang; Li, Chun-Xiu; Xu, Jian-He
, resulting in ca. 20-fold higher lipase ...
... transformation and one tube is for the  pUC18 
Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein–Cofactor and Protein–Protein Functional Relationships
Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein–Cofactor and Protein–Protein Functional Relationships
Sehanobish, Esha; Dow, Brian A.; Davidson, Victor L.
control.) Preheat NZY+ broth to 42 °C. ...
... below. Construction of a loxP–htk–loxP cassette  pUC18 
Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus
Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus
Togawa, Yoichiro; Nunoshiba, Tatsuo; Hiratsu, Keiichiro
–loxP–htk–loxP harboring a loxP–htk–loxP ...
... Kmr Novagen pHSG396 pMB1origin, Cmr Takara  pUC18 
Construction, properties, and application of the pCB5 plasmid, a novel conjugative shuttle vector with a Cupriavidus basilensis origin of replication
Construction, properties, and application of the pCB5 plasmid, a novel conjugative shuttle vector with a Cupriavidus basilensis origin of replication
Wang, Sheng; Zhang, Mei-Nan; Bai, Na-Ling; Ding, Hai-Tao; Zhu, Xu-Fen; Zhao, Yu-Hua
pMB1 origin, Ampr pK18mobsacB pMB1 origin mob + ...
... in pUC19, [in E. coli AmpR] Laboratory stock  pUC18 
Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
Brutesco, Catherine; Prévéral, Sandra; Escoffier, Camille; Descamps, Elodie C. T.; Prudent, Elsa; Cayron, Julien; Dumas, Louis; Ricquebourg, Manon; Adryanczyk-Perrier, Géraldine; de Groot, Arjan; Garcia, Daniel; Rodrigue, Agnès; Pignol, David; Ginet, Nicolas
-AmyEDD amyE (amylase), [AmpR] This study Table ...
... healthy rabbits. Constrution of transfer vector  pUC18 
Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone
Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone
Wu, Ying; Li, Yangguang; Wang, Mingshu; Sun, Kunfeng; Jia, Renyong; Chen, Shun; Zhu, Dekang; Liu, Mafeng; Yang, Qiao; Zhao, Xinxin; Chen, Xiaoyue; Cheng, Anchun
/EGFP-TKAB-BAC11 The plasmid pBeloBAC11 ...
... experiments. This vector was based on  pUC18 
Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis
Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis
Lu, Yifei; Yan, Hongxiang; Deng, Jiezhong; Huang, Zhigang; Jin, Xurui; Yu, Yanlan; Hu, Qiwen; Hu, Fuquan; Wang, Jing
added with a Ts replication origin RepA(Ts) ...
... evaporation. A total of 75 ng of commercial  pUC18 
Rapid and accurate early-stage detection of T-DNA/plant flanking sequences of resistant kumquats
Rapid and accurate early-stage detection of T-DNA/plant flanking sequences of resistant kumquats
Yang, Li; Chen, Manman; Shao, Nan; Shan, Shulei; Xu, Changjie; Guo, Weidong; Wang, Changchun
was simultaneously subjected to digestion with ...
... the desired genes were cloned into a  pUC18 
Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?
Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?
Kolesinski, Piotr; Rydzy, Malgorzata; Szczepaniak, Andrzej
vector (Thermo Fisher Scientific) or its ...
... an internal amplifications control based on  pUC18 
Detection and isolation of Shiga-Toxin producing Escherichia coli in flour in Germany between 2014 and 2017
Detection and isolation of Shiga-Toxin producing Escherichia coli in flour in Germany between 2014 and 2017
Mäde, Dietrich; Geuthner, Anne-Catrin; Imming, Rainer; Wicke, Amal
/pUC19. ...
... protein Omp31 was amplified, cloned into  pUC18 
Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells
Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells
Verdiguel-Fernández, L.; Oropeza-Navarro, R.; Basurto-Alcántara, Francisco J.; Castañeda-Ramírez, A.; Verdugo-Rodríguez, Antonio
plasmid, and inactivated by inserting a ...
... NdeI-HindIII, and cloned into the same sites of  pUC18 
Combining metabolomics and network analysis to improve tacrolimus production in Streptomyces tsukubaensis using different exogenous feedings
Combining metabolomics and network analysis to improve tacrolimus production in Streptomyces tsukubaensis using different exogenous feedings
Wang, Cheng; Liu, Jiao; Liu, Huanhuan; Liang, Shaoxiong; Wen, Jianping
, generating ...
... Reaction mixtures of 40 μl contained 0.1 μg  pUC18 
Evolution and function of the Mycoplasma hyopneumoniae peroxiredoxin, a 2-Cys-like enzyme with a single Cys residue
Evolution and function of the Mycoplasma hyopneumoniae peroxiredoxin, a 2-Cys-like enzyme with a single Cys residue
Gonchoroski, Taylor; Virginio, Veridiana G.; Thompson, Claudia E.; Paes, Jéssica A.; Machado, Cláudio X.; Ferreira, Henrique B.
supercoiled DNA, 25 mM Tris pH 7.5, 10 mM DTT, ...
... to the method of Wang et al. (2008). Plasmid  pUC18 
Characterization of LuxI and LuxR Protein Homologs of N-Acylhomoserine Lactone-Dependent Quorum Sensing System in Pseudoalteromonas sp. 520P1
Characterization of LuxI and LuxR Protein Homologs of N-Acylhomoserine Lactone-Dependent Quorum Sensing System in Pseudoalteromonas sp. 520P1
Dang, Hoang Tran; Komatsu, Shinya; Masuda, Hideyuki; Enomoto, Keiichi
and Escherichia coli DH5α were purchased from ...
... plasmid donor, CmR, KanR [40] Plasmids    pUC18 
Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus
Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus
Wang, Junhua; Wang, Cheng; Song, Kejing; Wen, Jianping
E. coli general cloning vector, AmpR [41] ...