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... nucleases were studied using supercoiled  pUC18 
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Synthesis, crystal structures, characterization and antitumor activities of two copper(II) complexes of a sulfonamide ligand
Hangan, Adriana Corina; Stan, Roxana Liana; Turza, Alexandru; Oprean, Luminiţa Simona; Páll, Emöke; Gheorghe-Cetean, Sînziana; Sevastre, Bogdan
DNA in cacodylate buffer (0.1 M, pH 6.0), in ...
... allow efficient cloning of the PCR product to  pUC18 
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization
Qin, Yanli; Wang, Yong-Xiang; Zhang, Jiming; Li, Jisu; Tong, Shuping
vector, whereas the internal BspQI sites allow ...
... from the pDONR 221 plasmid, cloned into the  pUC18 
pELMO, an optimised in-house cloning vector
pELMO, an optimised in-house cloning vector
Ramos, Andrea E.; Muñoz, Marina; Moreno-Pérez, Darwin A.; Patarroyo, Manuel A.
vector’s multiple cloning site (Thermo ...
... with the appropriate antibiotic. The pYES2,  pUC18 
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Huang, Xiao-mei; Li, Qing-qing; Chen, Xiu-ling; Fan, Jin-xia; Xu, Xiu-hong; Sun, Xu-dong; Li, Dong-yu; Zhao, Hong-xiao
, pPIC9K, and pCSN43 plasmids were used for the ...
... pIJ101 aac(3)IV ermEp; oriT RK2 [16] pL98 7.4  pUC18 
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Rebets, Yuriy; Kormanec, Jan; Luzhetskyy, Andriy; Bernaerts, Kristel; Anné, Jozef
aac(3)IV pIJ101 aac(3)IV ssrAp; oriT RK2 [16] ...
... (OD660 ≤ 1) may allow increased protein yields.   
Novel Tools for the Functional Expression of Metagenomic DNA
Novel Tools for the Functional Expression of Metagenomic DNA
Katzke, Nadine; Knapp, Andreas; Loeschcke, Anita; Drepper, Thomas; Jaeger, Karl-Erich
...
... transformation and one tube is for the  pUC18 
Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein–Cofactor and Protein–Protein Functional Relationships
Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein–Cofactor and Protein–Protein Functional Relationships
Sehanobish, Esha; Dow, Brian A.; Davidson, Victor L.
control.) Preheat NZY+ broth to 42 °C. ...
... Kmr Novagen pHSG396 pMB1origin, Cmr Takara  pUC18 
Construction, properties, and application of the pCB5 plasmid, a novel conjugative shuttle vector with a Cupriavidus basilensis origin of replication
Construction, properties, and application of the pCB5 plasmid, a novel conjugative shuttle vector with a Cupriavidus basilensis origin of replication
Wang, Sheng; Zhang, Mei-Nan; Bai, Na-Ling; Ding, Hai-Tao; Zhu, Xu-Fen; Zhao, Yu-Hua
pMB1 origin, Ampr pK18mobsacB pMB1 origin mob + ...
... in pUC19, [in E. coli AmpR] Laboratory stock  pUC18 
Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
Brutesco, Catherine; Prévéral, Sandra; Escoffier, Camille; Descamps, Elodie C. T.; Prudent, Elsa; Cayron, Julien; Dumas, Louis; Ricquebourg, Manon; Adryanczyk-Perrier, Géraldine; de Groot, Arjan; Garcia, Daniel; Rodrigue, Agnès; Pignol, David; Ginet, Nicolas
-AmyEDD amyE (amylase), [AmpR] This study Table ...
... evaporation. A total of 75 ng of commercial  pUC18 
Rapid and accurate early-stage detection of T-DNA/plant flanking sequences of resistant kumquats
Rapid and accurate early-stage detection of T-DNA/plant flanking sequences of resistant kumquats
Yang, Li; Chen, Manman; Shao, Nan; Shan, Shulei; Xu, Changjie; Guo, Weidong; Wang, Changchun
was simultaneously subjected to digestion with ...
... the desired genes were cloned into a  pUC18 
Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?
Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?
Kolesinski, Piotr; Rydzy, Malgorzata; Szczepaniak, Andrzej
vector (Thermo Fisher Scientific) or its ...
... Reaction mixtures of 40 μl contained 0.1 μg  pUC18 
Evolution and function of the Mycoplasma hyopneumoniae peroxiredoxin, a 2-Cys-like enzyme with a single Cys residue
Evolution and function of the Mycoplasma hyopneumoniae peroxiredoxin, a 2-Cys-like enzyme with a single Cys residue
Gonchoroski, Taylor; Virginio, Veridiana G.; Thompson, Claudia E.; Paes, Jéssica A.; Machado, Cláudio X.; Ferreira, Henrique B.
supercoiled DNA, 25 mM Tris pH 7.5, 10 mM DTT, ...
... to the method of Wang et al. (2008). Plasmid  pUC18 
Characterization of LuxI and LuxR Protein Homologs of N-Acylhomoserine Lactone-Dependent Quorum Sensing System in Pseudoalteromonas sp. 520P1
Characterization of LuxI and LuxR Protein Homologs of N-Acylhomoserine Lactone-Dependent Quorum Sensing System in Pseudoalteromonas sp. 520P1
Dang, Hoang Tran; Komatsu, Shinya; Masuda, Hideyuki; Enomoto, Keiichi
and Escherichia coli DH5α were purchased from ...
... previously (Sambrook and Russel 2001). The  pUC18 
Studies on the reactions of 3,2′-polymethylene-2-phenylbenzo[b]-1,10-phenanthrolines with Ru(tpy)Cl3 and properties of the products
Studies on the reactions of 3,2′-polymethylene-2-phenylbenzo[b]-1,10-phenanthrolines with Ru(tpy)Cl3 and properties of the products
Lu, Yang; Motiur Rahman, A. F. M.; Jahng, Yurngdong
plasmid was purchased from Bayou Biolabs and ...
... resistance mini-transposon [14] Plasmids  pUC18 
Knock-In-Leave-Behind (KILB): Genetic Grafting of Protease-Cleaving Sequences into Permissive Sites of Proteins with a Tn5-Based Transposition System
Knock-In-Leave-Behind (KILB): Genetic Grafting of Protease-Cleaving Sequences into Permissive Sites of Proteins with a Tn5-Based Transposition System
Calles, Belén; Lorenzo, Víctor
ApR, oriV ColE1, cloning vector (GenBank ...
... × 100 DNA cleavage The cleavage of supercoiled  pUC18 
Studies on some metal complexes of a quinoxaline based unsymmetrical ONNO donor ligand
Studies on some metal complexes of a quinoxaline based unsymmetrical ONNO donor ligand
Dhanaraj, C. Justin; Johnson, Jijo
DNA to its nicked circular and linear forms was ...
... of the ChiLCV genome The monomeric backbone (  pUC18 
Chilli leaf curl virus-based vector for phloem-specific silencing of endogenous genes and overexpression of foreign genes
Chilli leaf curl virus-based vector for phloem-specific silencing of endogenous genes and overexpression of foreign genes
Kushwaha, Nirbhay Kumar; Chakraborty, Supriya
-ChiLCV DNA A) of ChiLCV DNA A (GenBank ...
... about 200 ng of plasmid DNA (pBR322, Fermentas;  pUC18 
TfoI produced by Tepidimonas fonticaldi PL17, a moderate thermophilic bacterium, is an isoschizomer of MseI
TfoI produced by Tepidimonas fonticaldi PL17, a moderate thermophilic bacterium, is an isoschizomer of MseI
Kumar, Ravinder; Pinnaka, Anil Kumar; Krishnan, Beena
, Merck) and phage DNA (Lambda DNA, Takara or ...
... and OsPCS2b (700 bp) were cloned into  pUC18 
Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses
Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses
Das, Natasha; Bhattacharya, Surajit; Bhattacharyya, Somnath; Maiti, Mrinal K.
plasmid, and transformed into Escherichia coli ...
... used in this study Plasmid Genotype References  pUC18 
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
Meyer, Benjamin H.; Shams-Eldin, Hosam; Albers, Sonja-Verena
Cloning vector; Ampr Yanisch-Perron et al. ...
... bacteria and ampicillin-resistant E. coli dhpα-  pUC18 
Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars
Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars
Avetisyan, Arpi; Markosian, Anahit; Petrosyan, Margarit; Sahakyan, Naira; Babayan, Anush; Aloyan, Samvel; Trchounian, Armen
were used. ...
... and inserted into the SamI and PstI sites of  pUC18 
Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module
Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module
Liu, Yingmiao; Wang, Junzhi; Xu, Chongmao; Chen, Yong; Yang, Junjie; Liu, Dong; Niu, Huanqing; Jiang, Yu; Yang, Sheng; Ying, Hanjie
, producing pUC-PnpyrG carrying the natural ...
... harboring pIB139DR This study Plasmids    pUC18 
Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model
Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model
Dang, Lanqing; Liu, Jiao; Wang, Cheng; Liu, Huanhuan; Wen, Jianping
E. coli cloning vector; AmpR Our lab ...
... Cells, Growth Media 1. Parental vector (e.g.,  pUC18 
Analysis of Posttranscriptional Regulation of CCN Genes
Analysis of Posttranscriptional Regulation of CCN Genes
Kondo, Seiji; Kubota, Satoshi; Takigawa, Masaharu
, pcDNA3.1) for a double-step subcloning ...
... resistance gene was derived from  pUC18 
High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6
High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6
Kim, Taeyeon; Cho, Sukhyeong; Woo, Han Min; Lee, Sun-Mi; Lee, Jinwon; Um, Youngsoon; Seo, Jin-Ho
(Cho et al. 2013), which contains pMB1 origin ...
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