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... erm This study Plasmids    pUC18 
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production
Fu, Jing; Huo, Guangxin; Feng, Lili; Mao, Yufeng; Wang, Zhiwen; Ma, Hongwu; Chen, Tao; Zhao, Xueming
Ampr Lab Stock   ...
... on supercoiled covalently closed circular  pUC18 
Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions
Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions
Dizman, Yesim Akturk; Muratoglu, Hacer; Sandalli, Cemal; Nalcacioglu, Remziye; Demirbag, Zihni
DNA. ...
... 1.5 × 106 4 × 10−8 3 × 10−10 133.3 60Co γ–ray  pUC18 
DNA strand break dependence on Tris and arginine scavenger concentrations under ultra-soft X-ray irradiation: the contribution of secondary arginine radicals
DNA strand break dependence on Tris and arginine scavenger concentrations under ultra-soft X-ray irradiation: the contribution of secondary arginine radicals
Souici, Mounir; Khalil, Talat Tariq; Boulanouar, Omar; Belafrites, Abdelfettah; Mavon, Christophe; Fromm, Michel
29.75 ng µL−1 Tomita et al. (1995) 1.5 × 107 ...
... of Escherichia coli DH5α cells harbouring  pUC18 
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
Matos, Tiago; Mohamed, Elsayed T.; Queiroz, João A.; Bülow, Leif
in LB medium [26]. ...
... DNA in solution The interaction of HUTth with  pUC18 
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
Papageorgiou, Anna C.; Adam, Panagiotis S.; Stavros, Philemon; Nounesis, George; Meijers, Rob; Petratos, Kyriacos; Vorgias, Constantinos E.
plasmid DNA was performed by MST (Zillner et ...
... expression Datsenko and Wanner (2000)  pUC18 
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
Yang, Ying; Yang, Yi; Ou, Bingming; Xia, Pengpeng; Zhou, Mingxu; Li, Luan; Zhu, Guoqiang
Ampr, cloning vector Lab stock ...
... showed no products with the gDNA of Z. mays and  pUC18 
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Weihmann, Fabian; Eisermann, Iris; Becher, Rayko; Krijger, Jorrit-Jan; Hübner, Konstantin; Deising, Holger B.; Wirsel, Stefan G. R.
(Additional file 1A). ...
...   pKD4 Apr Kmr [18]   pETSPO Cmr MLSr [4]     pUC18 
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Croux, Christian; Nguyen, Ngoc-Phuong-Thao; Lee, Jieun; Raynaud, Céline; Saint-Prix, Florence; Gonzalez-Pajuelo, Maria; Meynial-Salles, Isabelle; Soucaille, Philippe
Apr Fermentas    ...
... of 50 μL at 37 °C for 2.5 h. Subsequently,  pUC18 
SeGSTo, a novel glutathione S-transferase from the beet armyworm (Spodoptera exigua), involved in detoxification and oxidative stress
SeGSTo, a novel glutathione S-transferase from the beet armyworm (Spodoptera exigua), involved in detoxification and oxidative stress
Xu, Pengfei; Han, Ningning; Kang, Tinghao; Zhan, Sha; Lee, Kwang Sik; Jin, Byung Rae; Li, Jianhong; Wan, Hu
super-coiled plasmid DNA (1000 ng) was added to ...
... relA1 Clontech, Heidelberg, Germany Plasmids  pUC18 
The response of Serratia marcescens JG to environmental changes by quorum sensing system
The response of Serratia marcescens JG to environmental changes by quorum sensing system
Sun, Shu-Jing; Liu, Hui-Jun; Weng, Cai-Hong; Lai, Chun-Fen; Ai, Liu-Ying; Liu, Yu-Chen; Zhu, Hu
pBR322-derived cloning vector, Lac+, Apr MBI ...
... concentrations: 3, 6, 9, 12 and 15 μM), 1 μL of  pUC18 
Synthesis, crystal structure and characterization of new biologically active Cu(II) complexes with ligand derived from N-substituted sulfonamide
Synthesis, crystal structure and characterization of new biologically active Cu(II) complexes with ligand derived from N-substituted sulfonamide
HANGAN, ADRIANA CORINA; TURZA, ALEXANDRU; STAN, ROXANA LIANA; SEVASTRE, BOGDAN; PÁLL, EMÖKE; CETEAN, SÎNZIANA; OPREAN, LUMINIŢA SIMONA
DNA solution (0.25 μg/ μL, 750 μM in base ...
... high copy number plasmids pLitmus38 (AMPr) and  pUC18 
Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level
Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level
Rohde, Alexander; Hammerl, Jens Andre; Al Dahouk, Sascha
(AMPr) or the medium copy number vector pBR328 ...
... U169 (Φ80 lacZ ΔM15) recA Takara Plasmids    pUC18 
The Development of Leucine Dehydrogenase and Formate Dehydrogenase Bifunctional Enzyme Cascade Improves the Biosynthsis of L-tert-Leucine
The Development of Leucine Dehydrogenase and Formate Dehydrogenase Bifunctional Enzyme Cascade Improves the Biosynthsis of L-tert-Leucine
Lu, Jixue; Zhang, Yonghui; Sun, Dongfang; Jiang, Wei; Wang, Shizhen; Fang, Baishan
-ldh Ampr, ldh expression cassette containing ...
... segments were synthesized and cloned in  pUC18 
Expression of an engineered synthetic cry2Aa (D42/K63F/K64P) gene of Bacillus thuringiensis in marker free transgenic tobacco facilitated full-protection from cotton leaf worm (S. littoralis) at very low concentration
Expression of an engineered synthetic cry2Aa (D42/K63F/K64P) gene of Bacillus thuringiensis in marker free transgenic tobacco facilitated full-protection from cotton leaf worm (S. littoralis) at very low concentration
Gayen, Srimonta; Mandal, Chandi Charan; Samanta, Milan Kumar; Dey, Avishek; Sen, Soumitra Kumar
vector according to Sambrook et al. (1989), and ...
... Methods Reagents and cell lines The HBV plasmid  pUC18 
Hepatitis B virus X protein promotes interleukin-7 receptor expression via NF-κB and Notch1 pathway to facilitate proliferation and migration of hepatitis B virus-related hepatoma cells
Hepatitis B virus X protein promotes interleukin-7 receptor expression via NF-κB and Notch1 pathway to facilitate proliferation and migration of hepatitis B virus-related hepatoma cells
Kong, Fanyun; Hu, Wei; Zhou, Kai; Wei, Xiao; Kou, Yanbo; You, Hongjuan; Zheng, Kuiyang; Tang, Renxian
-HBV1.2 (a vector containing HBV genome) and ...
... to erythromycin (Gryczan et al. 1980) Plasmids  pUC18 
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea
Wu, Hang; Li, Weiwei; Xin, Chen; Zhang, Congming; Wang, Yansheng; Ren, Shaohua; Ren, Min; Zhao, Wei; Yuan, Li; Xu, Zhongdong; Yuan, Hualing; Geng, Ming; Zhang, Lixin; Weaver, David T.; Zhang, Buchang
General cloning vector (Sambrook and Russell ...
... hydrogen formation and to produce more 1,3-PDO.   
Transcriptional Regulation of Genes Involved in 3-Hydroxypropionic Acid Production in Response to Aeration of Recombinant Klebsiella pneumoniae
Transcriptional Regulation of Genes Involved in 3-Hydroxypropionic Acid Production in Response to Aeration of Recombinant Klebsiella pneumoniae
Huang, Yanna; Li, Zhimin; Ye, Qin
...
... and driven by 35S promoter To make the modified  pUC18 
A WD40 protein, AtGHS40, negatively modulates abscisic acid degrading and signaling genes during seedling growth under high glucose conditions
A WD40 protein, AtGHS40, negatively modulates abscisic acid degrading and signaling genes during seedling growth under high glucose conditions
Hsiao, Yu-Chun; Hsu, Yi-Feng; Chen, Yun-Chu; Chang, Yi-Lin; Wang, Co-Shine
(Genemark) construct containing 35S promoter, ...
... al. (1990) Butyrivibrio fibrisolvens GS113 xylB  pUC18 
Molecular cloning and comparative sequence analysis of fungal β-Xylosidases
Molecular cloning and comparative sequence analysis of fungal β-Xylosidases
Mustafa, Ghulam; Kousar, Sumaira; Rajoka, Muhammad Ibrahim; Jamil, Amer
E. coli DH5α 60 Specific activity ...
... the PTGDR has been cloned in the polylinker. 5.  pUC18 
Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Marcos-Vadillo, Elena; García-Sánchez, Asunción
plasmid: Vector used as DNADNA carrier, to ...
... (M13RV tag). PCR was performed with the plasmid  pUC18 
Non-covalent Modification of Double-Stranded DNA at the Mismatch and Bulged Site
Non-covalent Modification of Double-Stranded DNA at the Mismatch and Bulged Site
Dohno, Chikara; Nakatani, Kazuhiko
as a template using the M13RV-tag primer and a ...
... −80 °C in 15 % glycerol. 2. PlasmidsPlasmids :  pUC18 
Isolation and Analysis of Suppressor Mutations in Tumor-Targeted msbB Salmonella
Isolation and Analysis of Suppressor Mutations in Tumor-Targeted msbB Salmonella
Low, K. Brooks; Murray, Sean R.; Pawelek, John; Bermudes, David
(Fisher Scientific, Waltham, MA) and pNK2883 ...
... was determined as the relaxation of supercoiled  pUC18 
Methyleugenol and oxidative metabolites induce DNA damage and interact with human topoisomerases
Methyleugenol and oxidative metabolites induce DNA damage and interact with human topoisomerases
Groh, Isabel Anna Maria; Rudakovski, Olga; Gründken, Malte; Schroeter, Anika; Marko, Doris; Esselen, Melanie
plasmid DNA according to Esselen et al. ...
... regions Synthesized through Genescript 2  pUC18 
Changing the Codon Usage of hfq Gene has Profound Effect on Phenotype and Pathogenicity of Salmonella Typhimurium
Changing the Codon Usage of hfq Gene has Profound Effect on Phenotype and Pathogenicity of Salmonella Typhimurium
Behera, Parthasarathi; Kutty, V. H. Muhammed; Kumar, Ajeet; Sharma, Bhaskar
...
... pHBV1.3 containing 1.3 copy of HBV genome in  pUC18 
HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells
HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells
Kong, Xiao-xiao; Lv, Yan-ru; Shao, Li-ping; Nong, Xiang-yang; Zhang, Guang-ling; Zhang, Yi; Fan, Hong-xia; Liu, Min; Li, Xin; Tang, Hua
) [26] were co-transfected into HBV-negative ...