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... [0.7 % (w/v)] showing the cleavage of  pUC18 
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine
Mendo, Ana Soraia; Figueiredo, Sara; Roma-Rodrigues, Catarina; Videira, Paula A.; Ma, Zhen; Diniz, Mário; Larguinho, Miguel; Costa, Pedro M.; Lima, João C.; Pombeiro, Armando J. L.; Baptista, Pedro V.; Fernandes, Alexandra R.
DNA (200 ng) incubated with ZM5 (50 μM) in the ...
...  pUC4K Apr, Kmr Taylor and Rose (1988)    pUC18 
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts
Nagayama, Hirofumi; Sugawara, Tomonori; Endo, Ryo; Ono, Akira; Kato, Hiromi; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
Apr, cloning vector pMB1ori Vieira and Messing ...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... Culture Collection (CICC). The plasmid  pUC18 
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Guo, Xuewu; Wang, Yazhou; Guan, Xiangyu; Chen, Yefu; Zhang, Cuiying; Xiao, Dongguang
 K were provided by Yu Zheng, associate ...
... expressions of DR4 and DR5, we transfected the  pUC18 
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
Kong, Fanyun; You, Hongjuan; Zhao, Jinjin; Liu, Wen; Hu, Lei; Luo, Wenya; Hu, Wei; Tang, Renxian; Zheng, Kuiyang
-HBV1.2, a plasmid contains the full-length HBV ...
... antibody. Materials and methods Materials The  pUC18 
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Hao, Chao-hui; Han, Qian-he; Shan, Zhong-jie; Hu, Jian-ting; Zhang, Nan; Zhang, Xue-pei
and pSectag2B plasmids, restriction ...
... cosmid was further constructed into a  pUC18 
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Wang, Jianjun; Zhu, Yaxin; Zhao, Guogang; Zhu, Junge; Wu, Sheng
plasmid library and screened by the same ...
... in an adverse manner. In this experiment,  pUC18 
Cloning and Transformation
Cloning and Transformation
Das, Surajit; Dash, Hirak Ranjan
and lambda DNA (λDNA) will be double digested ...
... Genomic insertion of eGFP This study Plasmids    pUC18 
A three-step method for analysing bacterial biofilm formation under continuous medium flow
A three-step method for analysing bacterial biofilm formation under continuous medium flow
Schmutzler, Karolin; Schmid, Andreas; Buehler, Katja
_mini-Tn7-Gm-T7 ...
... ΔldhA Markerless ldhA mutant This work Plasmids  pUC18 
Metabolic Analysis of a Corynebacterium glutamicumIdhA Mutant During an Efficient Succinate Production Using pH-Control Under Oxygen Deprivation
Metabolic Analysis of a Corynebacterium glutamicumIdhA Mutant During an Efficient Succinate Production Using pH-Control Under Oxygen Deprivation
Wang, Chen; Cai, Heng; Zhou, Zhihui; Wan, Hong-gui; Ouyang, Ping-kai
Apr; a-lac/MCS M13 ori Takara ...
... PCR fragments were cloned consecutively into  pUC18 
The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
Moritz, Katharina D.; Amendt, Birgit; Witt, Elisabeth M. H. J.; Galinski, Erwin A.
(Yanisch-Perron et al. 1985) resulting in the ...
... to conjugation experiments Biomedal Lifescience  pUC18 
Characterization of the molecular degradation mechanism of diphenyl ethers by Cupriavidus sp. WS
Characterization of the molecular degradation mechanism of diphenyl ethers by Cupriavidus sp. WS
Wang, Sheng; Bai, Naling; Wang, Bing; Feng, Zhuo; Hutchins, William C.; Yang, Ching-Hong; Zhao, Yuhua
Ampr pBasic Ampr, constructed from ...
... propionate), D2O, DNO3, NaOD, and  pUC18 
Interaction of methotrexate, an anticancer agent, with copper(II) ions: coordination pattern, DNA-cleaving properties and cytotoxic studies
Interaction of methotrexate, an anticancer agent, with copper(II) ions: coordination pattern, DNA-cleaving properties and cytotoxic studies
Nagaj, Justyna; Kołkowska, Paulina; Bykowska, Aleksandra; Komarnicka, Urszula K.; Kyzioł, Agnieszka; Jeżowska-Bojczuk, Małgorzata
plasmid DNA were obtained from Sigma-Aldrich ...
... scavenging properties. Effect of extract on  pUC18 
Leaf extract of cracker plant (Ruellia tuberosa Linn) induces metal chelating activity and DNA strands break: implications for its antioxidant-prooxidant property
Leaf extract of cracker plant (Ruellia tuberosa Linn) induces metal chelating activity and DNA strands break: implications for its antioxidant-prooxidant property
Singh, Mukesh; Dasgupta, Monikankana; Biswas, Soham
plasmid DNA was demonstrated by agarose gel ...
... human SP-C gene promoter (3.7 kb) cloned into  pUC18 
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Transgenically-expressed secretoglobin 3A2 accelerates resolution of bleomycin-induced pulmonary fibrosis in mice
Cai, Yan; Yoneda, Mitsuhiro; Tomita, Takeshi; Kurotani, Reiko; Okamoto, Minoru; Kido, Taketomo; Abe, Hiroyuki; Mitzner, Wayne; Guha, Arjun; Kimura, Shioko
vector with SV40 small T intron and poly A ...
... 2 μ-based multicopy vector, LEU2 Clontech    pUC18 
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Konishi, Jin; Fukuda, Akira; Mutaguchi, Kozue; Uemura, Takeshi
Cloning vector Takara Bio  pAUR135 Cloning ...
... with non-exposed supercoiled plasmid DNA (10 nM  pUC18 
UV radiation effects on a DNA repair enzyme: conversion of a [4Fe–4S]2+ cluster into a [2Fe–2S]2+
UV radiation effects on a DNA repair enzyme: conversion of a [4Fe–4S]2+ cluster into a [2Fe–2S]2+
Folgosa, Filipe; Camacho, Inês; Penas, Daniela; Guilherme, Márcia; Fróis, João; Ribeiro, Paulo A.; Tavares, Pedro; Pereira, Alice S.
, 2,686 bp) at different protein-pDNA ratios ...
... EDTA, 25 % (v/v) acetonitrile (see Note 7 ). 5.  pUC18 
SNP Genotyping by Heteroduplex Analysis
SNP Genotyping by Heteroduplex Analysis
Paniego, Norma; Fusari, Corina; Lia, Verónica; Puebla, Andrea
Hae III digested (Sigma Chemical Company, MO, ...
... used for subcloning and expression were  pUC18 
Backbone structures in human milk oligosaccharides: trans-glycosylation by metagenomic β-N-acetylhexosaminidases
Backbone structures in human milk oligosaccharides: trans-glycosylation by metagenomic β-N-acetylhexosaminidases
Nyffenegger, Christian; Nordvang, Rune Thorbjørn; Zeuner, Birgitte; Łężyk, Mateusz; Difilippo, Elisabetta; Logtenberg, Madelon J.; Schols, Henk A.; Meyer, Anne S.; Mikkelsen, Jørn Dalgaard
(Thermo Scientific, USA) and pETM-10 (EMBL), ...
... Agarose gel electrophoresis The plasmid  pUC18 
Syntheses of valpromide dipeptide derivatives and interactions of derivatives with ctDNA
Syntheses of valpromide dipeptide derivatives and interactions of derivatives with ctDNA
Zhao, Dongxin; Ma, Li; Lu, Kui; Wu, Jiangzhuo; He, Juan
commonly has three forms: supercoiled form, ...
... the lac promoter of commercially available  pUC18 
High-level expression of a recombinant active microbial transglutaminase in Escherichia coli
High-level expression of a recombinant active microbial transglutaminase in Escherichia coli
Salis, Barbara; Spinetti, Gaia; Scaramuzza, Silvia; Bossi, Michele; Saccani Jotti, Gloria; Tonon, Giancarlo; Crobu, Davide; Schrepfer, Rodolfo
vector (ATCC N° 37253) a coding sequence for ...
... This work  pMD-18 T TA-cloning vector Takara    pUC18 
DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea
DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea
Liao, Cheng-Heng; Xu, Ya; Rigali, Sébastien; Ye, Bang-Ce
-tsr ...
... (CT DNA; Bangalore Genie, India), supercoiled  pUC18 
DNA binding and cleavage activity of a structurally characterized Ni(II) Schiff base complex
DNA binding and cleavage activity of a structurally characterized Ni(II) Schiff base complex
KUMAR, SARAT CHANDRA; PAL, ABHIJIT; MITRA, MERRY; MANIKANDAMATHAVAN, V M; LIN, CHIA -HER; NAIR, BALACHANDRAN UNNI; GHOSH, RAJARSHI
DNA (Cesium chloride purified, Bangalore Genie, ...
... shuttle vector, CmR, EmR Haima et al. (1987)    pUC18 
Inverse metabolic engineering of Bacillus subtilis for xylose utilization based on adaptive evolution and whole-genome sequencing
Inverse metabolic engineering of Bacillus subtilis for xylose utilization based on adaptive evolution and whole-genome sequencing
Zhang, Bo; Li, Ning; Wang, Zhiwen; Tang, Ya-Jie; Chen, Tao; Zhao, Xueming
AmpR Yanisch-Perron et al. (1985)  pUCaraR ...