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... Mannheim, Germany). Subcloning into  pUC18 
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Debode, Frederic; Janssen, Eric; Berben, Gilbert
...
... into the SacI and SalI enzyme-cutting sites of  pUC18 
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Yari, Kheirollah; Afzali, Saboor; Mozafari, Hadi; Mansouri, Kamran; Mostafaie, Ali
cloning vector. ...
... DNA from plasmid  pUC18 
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Shiina, Takuya; Watanabe, Ritsuko; Shiraishi, Iyo; Suzuki, Masao; Sugaya, Yuki; Fujii, Kentaro; Yokoya, Akinari
was irradiated with low-LET (13 keV/μm) or ...
... the construction of the integration vector were  pUC18 
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Zhang, Huitu; Tian, Yao; Wang, Jianling; Li, Yonghai; Wang, Haikuan; Mao, Shuhong; Liu, Xiaoguang; Wang, Chunxia; Bie, Songtao; Lu, Fuping
and pUC57. ...
... et al. (1991a) pEBM2 8.0 pBL1 Kmr, lacZα  pUC18 
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Pátek, Miroslav; Nešvera, Jan
MCS Mobilizable Tauch et al. (1998) pMJ-Ab 5.2 ...
... (carrying wildtype dnaQ), and the empty vector  pUC18 
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Luan, Guodong; Cai, Zhen; Li, Yin; Ma, Yanhe
, was transformed into E. coli cells, ...
... that all complexes used converted supercoiled  pUC18 
Studies on synthesis, structure, and DNA cleaving of homo-dinuclear Mn(II), Cu(II), Ni(II), and Zn(II) complexes with a heterocycle-based macroacyclic Schiff base
Studies on synthesis, structure, and DNA cleaving of homo-dinuclear Mn(II), Cu(II), Ni(II), and Zn(II) complexes with a heterocycle-based macroacyclic Schiff base
Baran, Talat; Karaoğlu, Kaan; Serbest, Kerim; Ağır, Hülya; Sandallı, Cemal; Değirmencioğlu, İsmail
plasmid DNA to its nicked or linear form. ...
... Korea and used as a host for gene expression.  pUC18 
Enhanced activity of meso-secondary alcohol dehydrogenase from Klebsiella species by codon optimization
Enhanced activity of meso-secondary alcohol dehydrogenase from Klebsiella species by codon optimization
Lee, Soojin; Kim, Borim; Oh, Minkyu; Kim, Youngrok; Lee, Jinwon
(Takara Shuzo, Kyoto, Japan) was used as the ...
...  pPM Cmr, Emr; pHP13 (P43b ::mdh) This study    pUC18 
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Mu, Li; Wen, Jianping
E. coli cloning vector; Ampr Laboratory stock ...
... in this study Store in our lab [21] Plasmids    pUC18 
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Liao, Yan; Li, Chun-mei; Chen, Hui; Wu, Qi; Shan, Zhi; Han, Xue-yi
-phyA m Modified phyA fragment cloned into the ...
... Plasmid Characteristics Reference and source  pUC18 
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Kasuga, Kano; Nitta, Atsushi; Kobayashi, Masayuki; Habe, Hiroshi; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio; Kojima, Ikuo
/19 bla, lacZ′, pMB9 replicon Yanisch-Perron et ...
... digested with HindIII/PstI and ligated into the  pUC18 
Bm65 is Essential for the Propagation of Bombyx mori Nucleopolyhedrovirus
Bm65 is Essential for the Propagation of Bombyx mori Nucleopolyhedrovirus
Tang, Qi; Li, Guohui; Yao, Qin; Chen, Liang; Feng, Fan; Yuan, Yi; Chen, Keping
plasmid to generate the recombinant plasmid ...
... Genotype or descriptiona Source or reference  pUC18 
Canthaxanthin production with modified Mucor circinelloides strains
Canthaxanthin production with modified Mucor circinelloides strains
Papp, Tamás; Csernetics, Árpád; Nagy, Gábor; Bencsik, Ottó; Iturriaga, Enrique A.; Eslava, Arturo P.; Vágvölgyi, Csaba
General cloning vector for E. coli (Amp) ...
... from Shenshi Chem. Ltd. The plasmid DNA (  pUC18 
The Interaction of Polysaccharide from Auricularia polytricha with Quantum Dots and the Protection of Plasmid DNA from Damage
The Interaction of Polysaccharide from Auricularia polytricha with Quantum Dots and the Protection of Plasmid DNA from Damage
Wang, Wei; Zhang, Guoguang; Zou, Jinmei
) was extracted and purified from Escherichia ...
... CP-583, gltA:trc This study Plasmids    pUC18 
Directed pathway evolution of the glyoxylate shunt in Escherichia coli for improved aerobic succinate production from glycerol
Directed pathway evolution of the glyoxylate shunt in Escherichia coli for improved aerobic succinate production from glycerol
Li, Ning; Zhang, Bo; Chen, Tao; Wang, Zhiwen; Tang, Ya-jie; Zhao, Xueming
bla, pBR322 ori Lab collection  pEL04 cat-sacB ...
... I and Pst I and inserted into a cloning vector  pUC18 
Cofactor engineering through heterologous expression of an NADH oxidase and its impact on metabolic flux redistribution in Klebsiella pneumoniae
Cofactor engineering through heterologous expression of an NADH oxidase and its impact on metabolic flux redistribution in Klebsiella pneumoniae
Ji, Xiao-Jun; Xia, Zhi-Fang; Fu, Ning-Hua; Nie, Zhi-Kui; Shen, Meng-Qiu; Tian, Qian-Qian; Huang, He
, resulted in ...
... excised from the gel, purified, and cloned into  pUC18 
Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen
Bélanger, Louise; Charles, Trevor C
vector from which the insert DNA could be ...
... chloride was purchased from Qualigens. Plasmid  pUC18 
White light augments chemotherapeutic potential of cyclophosphamide: an in vitro study
White light augments chemotherapeutic potential of cyclophosphamide: an in vitro study
Chibber, Sandesh; Hassan, Iftekhar; Farhan, Mohd; Salman, Maria; Naseem, Imrana
was purchased from Genei, Banglore, India. ...
... was purified and cloned into the SmaI site of  pUC18 
Construction of an Expression Vector for Production and Purification of Human Somatostatin in Escherichia coli
Construction of an Expression Vector for Production and Purification of Human Somatostatin in Escherichia coli
Maicas, Sergi; Moukadiri, Ismaïl; Nieto, Almudena; Valentín, Eulogio
; the plasmid was named ...
... σ70 promoter M. Surette Kim and Surette 2006  pUC18 
The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
Herrington, Muriel B.; Sitaras, Chris
AmpR, colE1 ori C. ...
... restricted with 10 U of Sau3A, and cloned into  pUC18 
Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid
Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid
Wiesner, Magdalena; Fernández-Mora, Marcos; Cevallos, Miguel A; Zavala-Alvarado, Crispín; Zaidi, Mussaret B; Calva, Edmundo; Silva, Claudia
digested with BamHI using standard methods ...
... was digested with SalI/BamHI and cloned into  PUC18 
Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha
Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha
Wang, Hui; Song, Hao-lei; Wang, Qian; Qiu, Bing-sheng
to create the plasmid ...
... phnA4 from strain CHY-1 Jouanneau, unpublished  pUC18 
Diversity and catalytic potential of PAH-specific ring-hydroxylating dioxygenases from a hydrocarbon-contaminated soil
Diversity and catalytic potential of PAH-specific ring-hydroxylating dioxygenases from a hydrocarbon-contaminated soil
Martin, Florence; Malagnoux, Laure; Violet, Fabien; Jakoncic, Jean; Jouanneau, Yves
AmpR, cloning plasmid Yanisch-Perron et al. ...
... in this study Plasmid Description Reference pUT  pUC18 
Development of a Fur-dependent and tightly regulated expression system in Escherichia colifor toxic protein synthesis
Development of a Fur-dependent and tightly regulated expression system in Escherichia colifor toxic protein synthesis
Guan, Lingyu; Liu, Qin; Li, Chao; Zhang, Yuanxing
derivative with rrnBT1T2 terminator, Apr [18] ...
... [aac(3)IV] Blondelet-Rouault et al. (1997)    pUC18 
Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans
Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans
Guyet, Aurélie; Gominet, Myriam; Benaroudj, Nadia; Mazodier, Philippe
Cloning vector in E. coli Yanisch-Perron et al. ...