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... the RNA. Materials and methods DNA and RNA The  pUC18 
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... A fragment of the DNA sequence of the  pUC18 
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Golubev, S. S.; Kononogov, S. A.; Ravin, N. V.; Skryabin, K. G.
plasmid is proposed as a reference sequence for ...
... methods Reagents and biochemicals The plasmid  pUC18 
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Perry, Christopher C.; Urata, Sarah M.; Lee, Melissa; Aguilera, Joe A.; Milligan, Jamie R.
was grown on a liter scale in an E. coli host ...
... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... The libraries were developed in two vectors,  pUC18 
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Sharma, Nandita; Tanksale, Himgouri; Kapley, Atya; Purohit, Hemant J.
and pBeloBAC11, and efficacy was demonstrated ...
... pDONR™ P4-P1R, and pDONR™ P2R-P3 (see Note 1),  pUC18 
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Sone, Takefumi; Imamoto, Fumio
(8), template DNA for amplification of ...
... (1991)  CaMV35S-sGFP (S65T)-NOS3′ GFP in  pUC18 
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Kiyono, Masako; Oka, Yumiko; Sone, Yuka; Tanaka, Michitaka; Nakamura, Ryosuke; Sato, Masa H.; Pan-Hou, Hidemitsu; Sakabe, Kou; Inoue, Ken-ichiro
Uemura et al. (2004)  SYP111/KOLLE GFP-SYP111 ...
... of the elongation factor 1α (EF 1α) promoter.  pUC18 
shRNA-Mediated Gene Knockdown in Skeletal Muscle
shRNA-Mediated Gene Knockdown in Skeletal Muscle
Golzio, Muriel; Escoffre, Jean-Michel; Teissié, Justin
plasmid contains the cDNA of subunit α of LacZ ...
... GFP, the ORF was amplified by PCR and cloned in  pUC18 
AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family
AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family
Weichert, Annett; Brinkmann, Christopher; Komarova, Nataliya Y.; Dietrich, Daniela; Thor, Kathrin; Meier, Stefan; Suter Grotemeyer, Marianne; Rentsch, Doris
-spGFP6 and ...
... partially purified enzyme preparations using  pUC18 
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Gupta, Richa; Xu, Shuang-Yong; Sharma, Prince; Capalash, Neena
and/or λ DNA as substrates and Universal Buffer ...
... K. pneumoniae were cloned into the  pUC18 
Synthesis of Pure meso-2,3-Butanediol from Crude Glycerol Using an Engineered Metabolic Pathway in Escherichia coli
Synthesis of Pure meso-2,3-Butanediol from Crude Glycerol Using an Engineered Metabolic Pathway in Escherichia coli
Lee, Soojin; Kim, Borim; Park, Kyungmoon; Um, Youngsoon; Lee, Jinwon
plasmid and introduced into E. coli. In 2 l ...
... Fidelity PCR system (Roche). 7. Cloning vector  pUC18 
Generating Knock-Out and Complementation Strains of Neisseria meningitidis
Generating Knock-Out and Complementation Strains of Neisseria meningitidis
Dam, Vincent; Bos, Martine P.
(New England Biolabs) or pCRII-TOPO ...
... NIZO, Ede, The Netherlands. Plasmid  pUC18 
Re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of Listeria monocytogenes to β-lactam antibiotics
Re-evaluation of the significance of penicillin binding protein 3 in the susceptibility of Listeria monocytogenes to β-lactam antibiotics
Krawczyk-Balska, Agata; Popowska, Magdalena; Markiewicz, Zdzislaw
[24] was obtained from the collection of the ...
... and cloned into EcoRI-HindIII digested  pUC18 
Recombinatorial biosynthesis of polyketides
Recombinatorial biosynthesis of polyketides
Starcevic, Antonio; Wolf, Kerstin; Diminic, Janko; Zucko, Jurica; Ruzic, Ida Trninic; Long, Paul F.; Hranueli, Daslav; Cullum, John
[18] vector to give plasmid pKW40. ...
... pHT3101 ori pHT1030 (B. thuringiensis) ori  pUC18 
Shuttle Vectors of Bacillus thuringiensis
Shuttle Vectors of Bacillus thuringiensis
Ochoa-Zarzosa, Alejandra; López-Meza, Joel Edmundo
(E. coli) Lereclus et al. (1989) Low copy ...
... deoxyribonucleic acid (calf thymus), plasmid  pUC18 
Light-mediated interaction of methotrexate with transition metal Cu(II)
Light-mediated interaction of methotrexate with transition metal Cu(II)
Chibber, Sandesh; Hassan, Iftekhar; Farhan, Mohd; Naseem, Imrana
, BSA, and ethidium bromide were purchased from ...
... Spcr, Emr, Kmr, Tetr This study Plasmids  pUC18 
Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis
Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis
Li, Shanshan; Huang, Di; Li, Yong; Wen, Jianping; Jia, Xiaoqiang
E. coli cloning vector; Ampr Laboratory stock ...
... Inc. (MA, USA). Plasmid DNA preparation The  pUC18 
Effect of catechins and tannins on depleted uranium-induced DNA strand breaks
Effect of catechins and tannins on depleted uranium-induced DNA strand breaks
Matsuda, Emiko; Nakajima, Akira
plasmid DNA (Invitrogen Corp., CA, USA) was ...
... 5.109–2.1010 transformants/μg of supercoiled  pUC18 
Synthetic Customized scFv Libraries
Synthetic Customized scFv Libraries
Robin, Gautier; Martineau, Pierre
plasmid) required for the final library ...
... was obtained by PCR and was then cloned into  pUC18 
Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo
Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo
Gu, Jinyan; Zhang, Yu; Lian, Xue; Sun, Hailiang; Wang, Jingman; Liu, Weiting; Meng, Gang; Li, Peng; Zhu, Dan; Jin, Yuexin; Cao, Ruibing
digested with EcoR I to generate ...
... Tyramine producer IPLA Collection PLASMIDS  pUC18 
The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH
The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH
Linares, Daniel M; Fernández, Maria; Del-Río, Beatriz; Ladero, Victor; Martin, Maria Cruz; Alvarez, Miguel A
AmpR [39] pNZ9530 EryR, nisR-nisK [40] pILORI4 ...
... DNA protection was studied using supercoiled  pUC18 
Reducing power and iron chelating property of Terminalia chebula (Retz.) alleviates iron induced liver toxicity in mice
Reducing power and iron chelating property of Terminalia chebula (Retz.) alleviates iron induced liver toxicity in mice
Sarkar, Rhitajit; Hazra, Bibhabasu; Mandal, Nripendranath
plasmid DNA according to an earlier reported ...
... TAC125. The mesophilic signals consist of the  pUC18 
Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
Rippa, Valentina; Papa, Rosanna; Giuliani, Maria; Pezzella, Cinzia; Parrilli, Ermenegilda; Tutino, Maria Luisa; Marino, Gennaro; Duilio, Angela
-derived origin of replication (OriC) and a ...
... directly damages DNA in vitro, plasmid DNA of  pUC18 
Widdrol activates DNA damage checkpoint through the signaling Chk2–p53–Cdc25A–p21–MCM4 pathway in HT29 cells
Widdrol activates DNA damage checkpoint through the signaling Chk2–p53–Cdc25A–p21–MCM4 pathway in HT29 cells
Yun, Hee Jung; Hyun, Sook Kyung; Park, Jung Ha; Kim, Byung Woo; Kwon, Hyun Ju
(1.8 μg) and chromosomal DNA of HT29 cells ...
... Germany). EYFP cDNA was cloned into the plasmid  pUC18 
Biosynthesis of allene oxides in Physcomitrella patens
Biosynthesis of allene oxides in Physcomitrella patens
Scholz, Julia; Brodhun, Florian; Hornung, Ellen; Herrfurth, Cornelia; Stumpe, Michael; Beike, Anna K; Faltin, Bernd; Frank, Wolfgang; Reski, Ralf; Feussner, Ivo
-Entry [52] via PCR using primers with NotI and ...