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... of trimers) and a DNA substrate: i) undigested  pUC18 
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong; Watt, Rory M
(184 ng); ii) PstI-linearized ...
... with two XcmI cassettes was inserted into  pUC18 
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
Gu, Jingsong; Ye, Chunjiang
to construct an efficient XcmI T-vector parent ...
... for AFM For AFM imaging in air, 100.0 μg/ml  pUC18 
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
Shen, Xin-Cheng; Bao, Lei; Zhang, Zhi-Ling; Liu, Xiaoshen; Pang, Dai-Wen; Xu, Jianrong
DNA sample was diluted into final concentration ...
... fragment was subcloned into the HindIII site of  pUC18 
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Noda, Shuhei; Miyazaki, Takaya; Miyoshi, Takanori; Miyake, Michiru; Okai, Naoko; Tanaka, Tsutomu; Ogino, Chiaki; Kondo, Akihiko
. ...
... of STE2 encoding alpha-factor receptor, plasmid  pUC18 
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Liu, Jing-Jing; Ding, Wen-Tao; Zhang, Guo-Chang; Wang, Jing-Yu
-ste2-URA was constructed. ...
... NeoR, EGFP cloning vector Clontech pEMBL19  pUC18 
Arm site independence of coliphage HK022 integrase in human cells
Arm site independence of coliphage HK022 integrase in human cells
Malchin, Natalia; Tuby, Chen N.; Yagil, Ezra; Kolot, Mikhail
, cloning plasmid Yanisch-Perron et al. (1985) ...
... enzymes were purchased from TAKARA. Plasmids of  pUC18 
Cloning and Characterization of a Sucrose Isomerase from Erwinia rhapontici NX-5 for Isomaltulose Hyperproduction
Cloning and Characterization of a Sucrose Isomerase from Erwinia rhapontici NX-5 for Isomaltulose Hyperproduction
Li, Sha; Cai, Heng; Qing, Yujia; Ren, Ben; Xu, Hong; Zhu, Hongyang; Yao, Jun
and pET22b were obtained from Novagen Co., ...
... further cloning of the synthetic genes into  pUC18 
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Lamport, Derek T. A.; Tan, Li; Kieliszewski, Marcia J.
-SS tob -EGFP vector. ...
... and was subsequently used to generate the  pUC18 
Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek’s disease virus (MDV) alters expression of nearby MDV genes
Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek’s disease virus (MDV) alters expression of nearby MDV genes
Kim, Taejoong; Mays, Jody; Fadly, Aly; Silva, Robert F.
–RM1–LTR–kan transfer vector, using published ...
... much noisier. To achieve this, we sequenced a  pUC18 
Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study
Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study
Goto, Hiroki; Dickins, Benjamin; Afgan, Enis; Paul, Ian M; Taylor, James; Makova, Kateryna D; Nekrutenko, Anton
plasmid isolated from a single colony, which in ...
... ApR, cloning vector for E. coli Stratagene  pUC18 
Enhanced production of recombinant nattokinase in Bacillus subtilis by promoter optimization
Enhanced production of recombinant nattokinase in Bacillus subtilis by promoter optimization
Wu, Shu-Ming; Feng, Chi; Zhong, Jin; Huan, Lian-Dong
2.7 kb, ApR, cloning vector for E. coli ...
... was cloned into the corresponding sites of  pUC18 
Display of Organophosphorus Hydrolase on the Cyanobacterial Cell Surface Using Synechococcus Outer Membrane Protein A as an Anchoring Motif
Display of Organophosphorus Hydrolase on the Cyanobacterial Cell Surface Using Synechococcus Outer Membrane Protein A as an Anchoring Motif
Chungjatupornchai, Wipa; Kamlangdee, Attapon; Fa-aroonsawat, Sirirat
to obtain plasmid ...
... the Sfi 1 cloning cassette was cloned into  pUC18 
Site directed biotinylation of filamentous phage structural proteins
Site directed biotinylation of filamentous phage structural proteins
Smelyanski, Larisa; Gershoni, Jonathan M
expression vector (GeneScript USA Inc., ...
... et al. and Chen et al. labelled plasmid DNA (  pUC18 
The Role of Cell Wall Revealed by the Visualization of Saccharomyces cerevisiae Transformation
The Role of Cell Wall Revealed by the Visualization of Saccharomyces cerevisiae Transformation
Pham, Tuan Anh; Kawai, Shigeyuki; Kono, Emi; Murata, Kousaku
) with YOYO-1, incubated WT cell in the ...
... glnK gene this work pTZ19R ApR lacZ f1 IG [42]  pUC18 
Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1
Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1
Noindorf, Lilian; Bonatto, Ana C; Monteiro, Rose A; Souza, Emanuel M; Rigo, Liu U; Pedrosa, Fabio O; Steffens, Maria BR; Chubatsu, Leda S
ApR, lacZ, f1 Invitrogen pUCG08del 0.8 kb DNA ...
... gel. The 4CL fragments were subcloned into the  pUC18 
Isolation of RNA from Field-Grown Jute (Corchorus capsularis) Plant in Different Developmental Stages for Effective Downstream Molecular Analysis
Isolation of RNA from Field-Grown Jute (Corchorus capsularis) Plant in Different Developmental Stages for Effective Downstream Molecular Analysis
Samanta, Pradipta; Sadhukhan, Sanjoy; Das, Subrata; Joshi, Alpana; Sen, Soumitra K.; Basu, Asitava
vector following standard cloning method [18]. ...
... LSA34 Given by Dr. Zhaolan Mo  pUC-gapA  pUC18 
Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Zhao, Yan; Liu, Qin; Wang, Xiuhua; Zhou, Lingyun; Wang, Qiyao; Zhang, Yuanxing
derivative; Plac-gapA fusion, Apr This study ...
... EF695400). The PCR products were inserted into  pUC18 
Transgenic Peanut (Arachis hypogaea L.) Expressing the Urease Subunit B Gene of Helicobacter pylori
Transgenic Peanut (Arachis hypogaea L.) Expressing the Urease Subunit B Gene of Helicobacter pylori
Yang, Cheng-yun; Chen, Shuai-yin; Duan, Guang-cai
Vector (clone vector), resulting in ...
... leu2-3-112 lys 1-1 trp1-289 ura3-52). Plasmids  pUC18 
Hepatitis B virus X protein impedes the DNA repair via its association with transcription factor, TFIIH
Hepatitis B virus X protein impedes the DNA repair via its association with transcription factor, TFIIH
Qadri, Ishtiaq; Fatima, Kaneez; AbdeL-Hafiz, Hany
and pBR322 were used for repair synthesis ...
... provided by China Lion Brewery Group. Plasmid  pUC18 
Construction of recombinant industrial S. cerevisiae strain with barley lipid-transfer protein 1 secretion capability and lower PrA activity
Construction of recombinant industrial S. cerevisiae strain with barley lipid-transfer protein 1 secretion capability and lower PrA activity
Zhang, Hong-bo; Ruan, Hui; Li, Wei-fen; Zhang, Wei; Su, Zhong-rui; He, Guo-qing; Chen, Qi-he
was used as a vector for gene fragments ...
... to the authentic sfRNA, we then constructed  pUC18 
Small noncoding RNA modulates japanese encephalitis virus replication and translation in trans
Small noncoding RNA modulates japanese encephalitis virus replication and translation in trans
Fan, Yi-Hsin; Nadar, Muthukumar; Chen, Chiu-Chin; Weng, Chia-Chen; Lin, Yun-Tong; Chang, Ruey-Yi
/JEV(-)10976-10454 and used for generating ...
... T+ sac B+, gene repacement vector with MSC from  pUC18 
A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence
A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence
Goldová, Jana; Ulrych, Aleš; Hercík, Kamil; Branny, Pavel
(24) pPS858 ApR, GmR, gfp+, contains GmR-GFP ...
... pBI121 and pCAMBIA1301 and cloning vector  pUC18 
Agrobacterium tumefaciens Mediated Transformation of ChiV Gene to Trichoderma harzianum
Agrobacterium tumefaciens Mediated Transformation of ChiV Gene to Trichoderma harzianum
Yang, Liming; Yang, Qian; Sun, Kening; Tian, Ye; Li, Hulun
were used to successfully construct expression ...
... Relevant characteristics Reference Purpose  pUC18 
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense
Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense
Sant'Anna, Fernando H; Andrade, Dieime S; Trentini, Débora B; Weber, Shana S; Schrank, Irene S
cloning vector, ampr, lacZα Clontech cloning ...
... 9. Oligonucleotides with desired mutation. 10.  pUC18 
Site-Directed Mutagenesis
Site-Directed Mutagenesis
Carrigan, Patricia E.; Ballar, Petek; Tuzmen, Sukru
control plasmid for transformation control. 11. ...