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... provided by Prof. Gong Heng  K. pneumoniae/  pUC18 
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Zhao, Li; Zheng, Yu; Ma, Xingyuan; Wei, Dongzhi
K-dhaT Recombinant K. pneumoniae with plasmid ...
... μl) was performed with negatively supercoiled  pUC18 
A functional type I topoisomerase from Pseudomonas aeruginosa
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... by PCR. They were inserted into vector  pUC18 
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He; Hu, Nan
, yielding the recombinant expression vector ...
... the gene encoded in the plasmid substrates (  pUC18 
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan
: TS) for use in the cleavage reactions. (c) ...
... by standard PCR techniques from the template  pUC18 
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Caly, Leon; Jans, David A.; Piller, Sabine C.
-NL4.3 [15]. ...
... K12 and the recombinant strain E. coli JM101  pUC18 
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Sevcenco, Ana-Maria; Krijger, Gerard C.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Hagen, Wilfred R.; Hagedoorn, Peter-Leon
-azu [17] were used. ...
... as previously described [2] with either:  pUC18 
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Lee, David J; Bingle, Lewis EH; Heurlier, Karin; Pallen, Mark J; Penn, Charles W; Busby, Stephen JW; Hobman, Jon L
[17], 2,700 bp (high copy number plasmid), ...
... CA, USA). Nuclease activity assay Plasmid  pUC18 
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Wu, Shih-Lu; Li, Chia-Cheng; Chen, Jaw-Chyun; Chen, Yi-Jin; Lin, Ching-Ting; Ho, Tin-Yun; Hsiang, Chien-Yun
dsDNA, preparing with the Qiagen plasmid midi ...
... terminal repeat, and this vector was named  pUC18 
A novel fusion protein that functions as an enhanced green fluorescent protein reporter and a tetracycline-controlled transcriptional activator
A novel fusion protein that functions as an enhanced green fluorescent protein reporter and a tetracycline-controlled transcriptional activator
Hara, Kimiko; Kuwayama, Hisashi; Inukai, Yoshiaki; Yaginuma, Toshinobu; Niimi, Teruyuki
-piggyBacR. ...
... Engineering). Construction of rescue plasmid  pUC18 
Stimulation of bacterial DNA transformation by cattle saliva: implications for using genetically modified plants in animal feed
Stimulation of bacterial DNA transformation by cattle saliva: implications for using genetically modified plants in animal feed
Shedova, Ekaterina; Albrecht, Christiane; Zverlov, Vladimir V.; Schwarz, Wolfgang H.
/nptII The intact nptII DNA fragment (792 bp) ...
... tuberculosis genomic library was constructed in  pUC18 
Cloning and characterization of Rv0621 gene related to surfactant stress tolerance in Mycobacterium tuberculosis
Cloning and characterization of Rv0621 gene related to surfactant stress tolerance in Mycobacterium tuberculosis
Dan, Liao; Jianping, Xie; Ruzhen, Guo; Honghai, Wang
vector under the controlling of the inducible ...
... isolated from kimchi This study Plasmid       pUC18 
Identification of a replicon from pCC3, a cryptic plasmid from Leuconostoc citreum C4 derived from kimchi, and development of a new host–vector system
Identification of a replicon from pCC3, a cryptic plasmid from Leuconostoc citreum C4 derived from kimchi, and development of a new host–vector system
Chang, Ji Yoon; Chang, Hae Choon
2,686 bp, E. coli cloning vector, Apr, lacZ ...
... as control for transformation efficiency. The  pUC18 
Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells
Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells
Aachmann, Finn Lillelund; Aune, Trond Erik Vee
plasmid with ampicillin resistance marker ...
... and non-mobilizable multicopy plasmid (  pUC18 
Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?
Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?
Pérez-Mendoza, Daniel; de la Cruz, Fernando
::lacI q ). ...
... (Qiagen) and ligated to dephosphorylated  pUC18 
Heterologous Expression of Legumin Gene in E. coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)
Heterologous Expression of Legumin Gene in E. coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)
Gupta, Subodh; Mishra, Avinash; Verma, A. K.; Pandey, Ruchi; Chand, Laxmi
vector arms. ...
... site of plasmid pAJluc (a derivative of  pUC18 
Octamer and heat shock elements regulate transcription from the AcMNPV polyhedrin gene promoter
Octamer and heat shock elements regulate transcription from the AcMNPV polyhedrin gene promoter
Kumar, M. Senthil; Ramachandran, Aruna; Hasnain, Seyed E.; Bashyam, Murali Dharan
carrying the 1,892-bp luc gene [19] ligated at ...
... cells/ml), chrysotile (50 µg/ml), NaCl, and  pUC18 
Plasmid uptake by bacteria: a comparison of methods and efficiencies
Plasmid uptake by bacteria: a comparison of methods and efficiencies
Yoshida, Naoto; Sato, Misa
donor plasmid (10–100 ng/ml) was spread onto a ...
... The gfp cassette was obtained by digesting  pUC18 
Characterization of Bombyx mori nucleopolyhedrovirus orf74, a novel gene involved in virulence of virus
Characterization of Bombyx mori nucleopolyhedrovirus orf74, a novel gene involved in virulence of virus
Shen, Hongxing; Chen, Keping; Yao, Qin; Yu, Wei; Pan, Ye; Huo, Juan; Xia, Hengchuan; Huang, Guoping
-gfp with XhoI and HindIII, and was subcloned ...
... used as host of the cloning experiments, and  pUC18 
Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate
Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate
Wei, Ping; Bai, Liping; Song, Wengang; Hao, Gangping
was employed as vector. ...
... bp EcoR I-Kpn I fragment A of tatA cloned into  pUC18 
Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae
Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae
Zhang, Lijuan; Zhu, Zhaoqin; Jing, Huaiqi; Zhang, Jingyun; Xiong, Yanwen; Yan, Meiying; Gao, Shouyi; Wu, Long-Fei; Xu, Jianguo; Kan, Biao
This study pT2 461 bp Xba I-Pst I fragment B of ...
... directional cloning of these sequences in  pUC18 
Expression of sunflower cytoplasmic male sterility-associated open reading frame, orfH522 induces male sterility in transgenic tobacco plants
Expression of sunflower cytoplasmic male sterility-associated open reading frame, orfH522 induces male sterility in transgenic tobacco plants
Nizampatnam, Narasimha Rao; Doodhi, Harinath; Kalinati Narasimhan, Yamini; Mulpuri, Sujatha; Viswanathaswamy, Dinesh Kumar
. ...
... Expression host, Camr (Chloramphenicol) Novagen  pUC18 
Genetic and biochemical analyses of chlorobenzene degradation gene clusters in Pandoraea sp. strain MCB032
Genetic and biochemical analyses of chlorobenzene degradation gene clusters in Pandoraea sp. strain MCB032
Jiang, Xi-Wen; Liu, Hong; Xu, Ying; Wang, Shu-Jun; Leak, David J.; Zhou, Ning-Yi
Cloning vector, Ampr (Ampicillin) Takara pET5a ...
... al. (2005), Quenee et al. (2005) Plasmid       pUC18 
Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440
Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440
Yang, Yao; Yuan, Sheng; Chen, Ting; Ma, Pengjuan; Shang, Guangdong; Dai, Yijun
Cloning vector. ...
... + sacB +, gene replacement vector with MCS from  pUC18 
The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa
The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa
Gliese, Nicole; Khodaverdi, Viola; Görisch, Helmut
Hoang et al. (1998)   pLAFR3 Tcr; ...
... preserved in our laboratory. E. coli JM109 and  pUC18 
Functional analysis of active site residues of Bacillus thuringiensis WB7 chitinase by site-directed mutagenesis
Functional analysis of active site residues of Bacillus thuringiensis WB7 chitinase by site-directed mutagenesis
Cai, Wenxuan; Sha, Li; Zhou, Jiawei; Huang, Zhipeng; Guan, Xiong
vector (Stratagene) were used for recombinant ...