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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... − lacZ − was cotransformed with various PutA-  pUC18 
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli
Zhou, Yuzhen; Zhu, Weidong; Bellur, Padmanetra S.; Rewinkel, Dustin; Becker, Donald F.
constructs (PutA- ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...
... feces (serotype O1483: H1) ATCC Plasmids    pUC18 
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Zhou, Lingyun; Liu, Qin; Wang, Qiyao; Ma, Yue; Xu, Yuzhou; Yang, Zhao; Zhao, Yan; Zhang, Yuanxing
Cloning/expression vector, Apr lacZ-α, Ori: ...
... per recipient cell (t/r) using plasmid  pUC18 
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Rizzi, Aurora; Brusetti, Lorenzo; Arioli, Stefania; Nielsen, Kaare M.; Tamagnini, Isabella; Tamburini, Alberto; Sorlini, Claudia; Daffonchio, Daniele
or a 1,873 bp fragment as donor DNA, and 3.5 × ...
... pSVJ21, was constructed by ligating p54 and  pUC18 
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Miteva, Vanya; Lantz, Sarah; Brenchley, Jean
and inserting a chloramphenicol acetyl ...
... was conducted as previously described [17, 27].  pUC18 
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Jeong, Kwang Cheol; Hung, Kai Foong; Baumler, David J; Byrd, Jeffrey J; Kaspar, Charles W
plasmid DNA (300 ng) was mixed with varying ...
... E. coli cloning vector, 4.3 kb TaKaRa     pUC18 
Role of DNA in Bacterial Aggregation
Role of DNA in Bacterial Aggregation
Liu, Hui-Hui; Yang, Yi-Ran; Shen, Xin-Cheng; Zhang, Zhi-Ling; Shen, Ping; Xie, Zhi-Xiong
E. coli cloning vector, 2.7 kb TaKaRa   Herring ...
... with nitrogen for 15 min prior to measurement.  pUC18 
Evaluation of antiproliferative activities and apoptosis induction caused by copper(II)–benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cells
Evaluation of antiproliferative activities and apoptosis induction caused by copper(II)–benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cells
González-Álvarez, Marta; Alzuet, Gloria; Borrás, Joaquín; Castillo-Agudo, Lucas; Montejo-Bernardo, Jose Manuel; Gutiérrez-Rodríguez, Angel; García-Granda, Santiago
DNA cleavage Reactions were performed by mixing ...
... for construction of GFP derivatives. Plasmid  pUC18 
Surface Display of GFP by Pseudomonas Syringae Truncated Ice Nucleation Protein in Attenuated Vibrio Anguillarum Strain
Surface Display of GFP by Pseudomonas Syringae Truncated Ice Nucleation Protein in Attenuated Vibrio Anguillarum Strain
Xu, Yuzhou; Liu, Qin; Zhou, Lingyun; Yang, Zhao; Zhang, Yuanxing
(TaKaRa, Japan) was used as a parent plasmid ...
... any plasmid encoding the LacZα-peptide such as  pUC18 
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Refaii, Abdalla Al; Jean-Hervé, Alix
/19 or pWSK129 (13) can be used. ...
... and Rp107; Additional file 4) and the plasmid  pUC18 
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Hwang, Yeen Ting; McCartney, Andrew W; Gidda, Satinder K; Mullen, Robert T
/CIRV (purchased from the American Type Culture ...
... Relevant characteristics Reference Plasmids    pUC18 
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
The 5′ untranslated region of fruA mRNA is required for translational enhancement of FruA synthesis during Myxococcus xanthus development
Ding, Nianhua; Zheng, Ying; Wu, Qian; Mao, Xiaohua
Cloning vector, ApR Yanisch-Perron et al. ...
... the ligands and their complexes were assayed on  pUC18 
Synthesis, characterization, DNA binding and nuclease activity of binuclear copper(II) complexes of cuminaldehyde thiosemicarbazones
Synthesis, characterization, DNA binding and nuclease activity of binuclear copper(II) complexes of cuminaldehyde thiosemicarbazones
Murali Krishna, Panchangam; Hussain Reddy, Katreddi; Pandey, Jay Prakash; Siddavattam, Dayananda
plasmid DNA using gel electrophoresis in the ...
... buffers (New England BioLabs, Beverly, MA) 2.  pUC18 
Construction of Infectious Clones for RNA Viruses: TMV
Construction of Infectious Clones for RNA Viruses: TMV
Chapman, Sean N.
plasmid (Fermentas, Hanover, MD) (see note 7) ...
... elements from -156 to +195 was cloned into  pUC18 
EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection
EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection
Eilebrecht, Sebastian; Pellay, François-Xavier; Odenwälder, Peter; Brysbaert, Guillaume; Benecke, Bernd-Joachim; Benecke, Arndt
. ...
... screening program [22]. Escherichia coli DH5α/  pUC18 
Optimization of Surfactin Production by Bacillus subtilis Isolate BS5
Optimization of Surfactin Production by Bacillus subtilis Isolate BS5
Abdel-Mawgoud, A. Mohammad; Aboulwafa, M. Mabrouk; Hassouna, Nadia Abdel-Haleem
(Hanahan 1983; Vieira and Messing 1982) was ...
... and subcloned into the EcoR I–Hind III site of  pUC18 
Cloning and Characterization of the DHDPS Gene Encoding the Lysine Biosynthetic Enzyme Dihydrodipocolinate Synthase from Zizania latifolia (Griseb)
Cloning and Characterization of the DHDPS Gene Encoding the Lysine Biosynthetic Enzyme Dihydrodipocolinate Synthase from Zizania latifolia (Griseb)
Kong, Fanna; Jiang, Shaomei; Meng, Xiangbing; Song, Chengli; Shi, Jinfeng; Jin, Demin; Jiang, Shaolin; Wang, Bin
. ...
... Biotech Company (Shanghai). pMD18-T vector,  pUC18 
Construction of a Rhizopus arrhizus glucoamylase gene suitable for expression in distinct host: introns spliced artificially by PCR
Construction of a Rhizopus arrhizus glucoamylase gene suitable for expression in distinct host: introns spliced artificially by PCR
Yang, Liquan; Dai, Xiaojun; Hou, Jianhua; Ma, Chunxiao; Wang, Cuiyan; Wu, Zhiqiang; Li, Minggang
vector, T4 DNA Ligase, and soluble starch are ...
... sites were used to clone the fragment into  pUC18 
Gene deletion and allelic replacement in the filamentous fungus Podospora anserina
Gene deletion and allelic replacement in the filamentous fungus Podospora anserina
El-Khoury, Riyad; Sellem, Carole H.; Coppin, Evelyne; Boivin, Antoine; Maas, Marc F. P. M.; Debuchy, Robert; Sainsard-Chanet, Annie
. ...
... kit (Bioflux). Cloning of Hoxa7 The plasmid  pUC18 
An Alternative Approach to Synthesize cDNA Bypassing Traditional Reverse Transcription
An Alternative Approach to Synthesize cDNA Bypassing Traditional Reverse Transcription
Li, Xiao-Xia; Zheng, Fang; Jiao, Yan-Li; Guo, Gang; Wang, Bao-Li; Yao, Zhi
was digested using HindIII and EcoRI and ...
... No abstract available Small (pUC12/13 1680 bp,  pUC18 
pUC Vectors
pUC Vectors
Rédei, George P.
/19 2686 bp) plasmids containing the ...
... 2). Construction of the Genomic Libraries 1.  pUC18 
Investigating the Mode of Action of Proline-Rich Antimicrobial Peptides Using a Genetic Approach: A Tool to Identify New Bacterial Targets Amenable to the Design of Novel Antibiotics
Investigating the Mode of Action of Proline-Rich Antimicrobial Peptides Using a Genetic Approach: A Tool to Identify New Bacterial Targets Amenable to the Design of Novel Antibiotics
Scocchi, Marco; Mattiuzzo, Maura; Benincasa, Monica; Antcheva, Nikolinka; Tossi, Alessandro; Gennaro, Renato
plasmid. 2. BamHI (10 U/ $\upmu$ L), Sau3AI (10 ...
... under 35S promoter were inserted into the  pUC18 
Transient expression of AtNCED3 and AAO3 genes in guard cells causes stomatal closure in Vicia faba
Transient expression of AtNCED3 and AAO3 genes in guard cells causes stomatal closure in Vicia faba
Melhorn, Vanessa; Matsumi, Kunimi; Koiwai, Hanae; Ikegami, Keiichi; Okamoto, Masanori; Nambara, Eiji; Bittner, Florian; Koshiba, Tomokazu
(35S::GFP ...
... was ligated into the XabI and HindIII sites of  pUC18 
Excision of the nifD element in the heterocystous cyanobacteria
Excision of the nifD element in the heterocystous cyanobacteria
Henson, B. J.; Pennington, L. E.; Watson, L. E.; Barnum, S. R.
, creating pRE-18. pDK1 and pDK3 The plasmid ...