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... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... of the 3′-end region of the GPD1. The plasmid  pUC18 
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Kong, Qing-Xue; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun
-R was created by inserting a 446-bp of ...
... and Chemicals pBluescript II KS (–),  pUC18 
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong
, and pIJ2925 were used for routine cloning and ...
... containing a large spacer DNA sequence of  pUC18 
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
Wang, Baoli; Li, Xiaoxia; Zheng, Fang; Liu, Rui; Quan, Jinxing; Liang, Hui; Guo, Shanyi; Guo, Gang; Zhang, Jingyu
. ...
... salinarum bearing pHH205 [29] Plasmids     pUC18 
Identification Homologous Recombination Function from Haloarchaea Plasmid pHH205
Identification Homologous Recombination Function from Haloarchaea Plasmid pHH205
Mei, Yunjun; Chen, Dong; Sun, Dongchang; Wang, Xiaojuan; Huang, Yuping; Chen, Xiangdong; Shen, Ping
Rep(pMB1) AmpR Laboratory reserved   pWL102 ...
... tumefaciens) with shaking (200 rpm). Plasmid  pUC18 
Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate
Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate
Kahrizi, Danial; Salmanian, Ali Hatef; Afshari, Afsoon; Moieni, Ahmad; Mousavi, Amir
(MBI, Fermentas) was used for routine cloning ...
... and screened two subclones in E. coli. One is  pUC18 
Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli
Identification of promoters of two dehydrogenase genes in Ketogulonicigenium vulgare DSM 4025 and their strength comparison in K. vulgare and Escherichia coli
Fu, Shulin; Zhang, Weicai; Guo, Aiguang; Wang, Jianhua
-SDH, which contained sdh; another is ...
... present study Plasmid Relevant genotype Source  pUC18 
N terminus determinants of MinC from Neisseria gonorrhoeae mediate interaction with FtsZ but do not affect interaction with MinD or homodimerization
N terminus determinants of MinC from Neisseria gonorrhoeae mediate interaction with FtsZ but do not affect interaction with MinD or homodimerization
Greco-Stewart, V.; Ramirez-Arcos, S.; Liao, M.; Dillon, J. R.
AmpRPlac Amersham Pharmacia pSR2 AmpRPlac::minC ...
... La Jolla, CA); pCMV-Sport6-Empty and  pUC18 
A functional map of NFκB signaling identifies novel modulators and multiple system controls
A functional map of NFκB signaling identifies novel modulators and multiple system controls
Halsey, Thomas A; Yang, Longlong; Walker, John R; Hogenesch, John B; Thomas, Russell S
-Empty (Invitrogen); pCS2-DsRed (kindly ...
... then inserted into the corresponding sites of  pUC18 
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli
Choi, Seon-Kang; Harada, Hisashi; Matsuda, Satoru; Misawa, Norihiko
vector (Toyobo) to, respectively, construct ...
... or description Source E. coli originated  pUC18 
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Analysis of the 7.6-kb cryptic plasmid pNC500 from Rhodococcus rhodochrous B-276 and construction of RhodococcusE. coli shuttle vector
Matsui, Toru; Saeki, Hisashi; Shinzato, Naoya; Matsuda, Hitoshi
Cloning vector, Apr Takara R. rhodochrous ...
... was cloned and expressed in E. coli using a  pUC18 
A point mutation changes the serotype of a Potato virus Y isolate; genomic determination of the serotype of PVY strains
A point mutation changes the serotype of a Potato virus Y isolate; genomic determination of the serotype of PVY strains
Chikh Ali, Mohamad; Maoka, Tetsuo; Natsuaki, Keiko T.
vector. ...
... of chloramphenicol resistance gene; Cmr [14]    pUC18 
Inactivation of type I polyhydroxyalkanoate synthase in Aeromonas hydrophila resulted in discovery of another potential PHA synthase
Inactivation of type I polyhydroxyalkanoate synthase in Aeromonas hydrophila resulted in discovery of another potential PHA synthase
Hu, Fengqing; You, Song
Suicide plasmid. ...
... was then transferred as an EcoRI fragment in  pUC18 
Cloning of the Arthrobacter sp. FG1 dehalogenase genes and construction of hybrid pathways in Pseudomonas putida strains
Cloning of the Arthrobacter sp. FG1 dehalogenase genes and construction of hybrid pathways in Pseudomonas putida strains
Radice, Francesca; Orlandi, Viviana; Massa, Valentina; Battini, Vanessa; Bertoni, Giovanni; Reineke, Walter; Barbieri, Paola
(Vieiria and Messing 1982) and in the ...
... gene cloned on an SphI fragment This work    pUC18 
Distinct mechanisms regulate expression of the two major groEL homologues in Rhizobium leguminosarum
Distinct mechanisms regulate expression of the two major groEL homologues in Rhizobium leguminosarum
Gould, Phillip; Maguire, Maria; Lund, Peter A.
ApR; cloning vector Yanisch-Perron et al. ...
... Plasmid and mutant construction Plasmid  pUC18 
Over-expressing GLT1 in a gpd2Δ mutant of Saccharomyces cerevisiae to improve ethanol production
Over-expressing GLT1 in a gpd2Δ mutant of Saccharomyces cerevisiae to improve ethanol production
Kong, Qing-Xue; Zhang, Ai-Li; Cao, Li-Min; Chen, Xun
-RYUR containing a gene disruption cassette has ...
... replicon; cos Mob+, Tcr Kimbara et al. (1989)    pUC18 
Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205
Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from γ-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205
Ito, Michihiro; Prokop, Zbyňek; Klvaňa, Martin; Otsubo, Yoshiyuki; Tsuda, Masataka; Damborský, Jiří; Nagata, Yuji
pMB9 replicon; Apr Vieira and Messing (1982) ...
... to the vectors pDrive (Qiagen, Hilden) and  pUC18 
FarR, a putative regulator of amino acid metabolism in Corynebacterium glutamicum
FarR, a putative regulator of amino acid metabolism in Corynebacterium glutamicum
Hänßler, Eva; Müller, Tim; Jeßberger, Nadja; Völzke, Anja; Plassmeier, Jens; Kalinowski, Jörn; Krämer, Reinhard; Burkovski, Andreas
(Viera and Messing 1982), respectively, ...
... from T. fusca NTU22, Escherichia coli DH5α and  pUC18 
Cloning and characterization of a maltotriose-producing α-amylase gene from Thermobifida fusca
Cloning and characterization of a maltotriose-producing α-amylase gene from Thermobifida fusca
Yang, Chao-Hsun; Liu, Wen-Hsiung
(Boehringer Mannheim, Germany) were used as the ...
... sp. FLA in BglII site of pIJ702 This study  pUC18 
A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols
A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols
Merkens, Hedda; Sielker, Sonja; Rose, Karsten; Fetzner, Susanne
ColE1 lacZ, bla Vieira and Messing (1982) ...
... nonpolar mutants using the cloned genes and  puc18 
Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae
Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae
Lee, Sang-Won; Ronald, Pamela C.
, and complement mutants with the cloned genes ...
... and ligated into the EcoRI and BamHI site of  pUC18 
The functional alteration of mutant GFAP depends on the location of the domain: morphological and functional studies using astrocytoma-derived cells
The functional alteration of mutant GFAP depends on the location of the domain: morphological and functional studies using astrocytoma-derived cells
Yoshida, Tomokatsu; Tomozawa, Yasuko; Arisato, Takayo; Okamoto, Yuji; Hirano, Hirofumi; Nakagawa, Masanori
plasmid (Takara Shuzo, Otsu, Japan). ...
... now GE Healthcare) and inserted into the  pUC18 
In situdetection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS
In situdetection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS
Stougaard, Magnus; Lohmann, Jakob S; Zajac, Magdalena; Hamilton-Dutoit, Stephen; Koch, Jørn
plasmid (Fermentas) using the restriction ...
... The arc gene (encoding ARC), located on a  pUC18 
Proteome analysis of Streptomyces coelicolor mutants affected in the proteasome system reveals changes in stress-responsive proteins
Proteome analysis of Streptomyces coelicolor mutants affected in the proteasome system reveals changes in stress-responsive proteins
De Mot, René; Schoofs, Geert; Nagy, István
-cloned 8233-bp SphI genomic fragment ...
... al. 2005). A hybrid plasmid, pRM100 composed of  pUC18 
Cloning and expression of heterologous genes in Rhodothermus marinus
Cloning and expression of heterologous genes in Rhodothermus marinus
Bjornsdottir, Snaedis H.; Fridjonsson, Olafur H.; Kristjansson, Jakob K.; Eggertsson, Gudmundur
and the cryptic R. marinus plasmid pRM21, was ...