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... Knabc We constructed an expression vector from  pUC18 
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Kurz, Matthias; Brünig, Anika NS; Galinski, Erwin A
where the β-lactamase is replaced in-frame by ...
... inactivation of glnA3; aprar This work  pUC18 
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... from Novagen (Darmstadt, Germany). The plasmid  pUC18 
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst
Liu, D.; Schmid, R. D.; Rusnak, M.
was purchased from MBI Fermentas (St. ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... Sisco Research Laboratories, Mumbai, India.  pUC18 
Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.)
Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.)
Ani, V.; Varadaraj, M. C.; Naidu, K. Akhilender
DNA was purchased from Bangalore Genei, ...
... along with chrysotile asbestos fibers and  pUC18 
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Yoshida, Naoto; Takebe, Kazuki
donor DNA, was spread over the surface of an LB ...
... a double cos cassette Keen et al. 1988    pUC18 
Plasmid-mediated degradation of 4-chloronitrobenzene by newly isolated Pseudomonas putida strain ZWL73
Plasmid-mediated degradation of 4-chloronitrobenzene by newly isolated Pseudomonas putida strain ZWL73
Zhen, Da; Liu, Hong; Wang, Shu-Jun; Zhang, Jun-Jie; Zhao, Fei; Zhou, Ning-Yi
Cloning vector, Ampr TaKaRa  pET5a Expression ...
... of a recombinant plasmid pFUC (a derivative of  pUC18 
Preliminary characterization of a thermostable DNA polymerase I from a mesophilic Bacillus sphaericus strain C3-41
Preliminary characterization of a thermostable DNA polymerase I from a mesophilic Bacillus sphaericus strain C3-41
Bei, Han; Haizhou, Liu; Xiaomin, Hu; Zhiming, Yuan
with a DNA insert) was used as DNA template and ...
... at 37°C in Luria Bertani (LB) medium. Plasmids  pUC18 
Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T
Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T
Zhao, B.; Lu, W.; Yang, L.; Zhang, B.; Wang, L.; Yang, S. S.
and pGEM T-easy vector were used in cloning and ...
... in Populus sp. plants, was cloned into  pUC18 
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Murata, Hitoshi; Sunagawa, Masahide; Yamazaki, Takashi; Shishido, Kazuo; Igasaki, Tomohiro
, generating pHHM181. dsred2 was newly ...
... [49] pLAF-csaR pLAFR3 carrying csaR [49]  pUC18 
Quorum Sensing and Phenazines are Involved in Biofilm Formation by Pseudomonas chlororaphis (aureofaciens) Strain 30-84
Quorum Sensing and Phenazines are Involved in Biofilm Formation by Pseudomonas chlororaphis (aureofaciens) Strain 30-84
Maddula, V. S. R. K.; Zhang, Z.; Pierson, E. A.; Pierson, L. S., III
-csaI ...
... for E. coli/Streptomyces conjugation [20]  pUC18 
Identification of AHBA Biosynthetic Genes Related to Geldanamycin Biosynthesis in Streptomyces hygroscopicus 17997
Identification of AHBA Biosynthetic Genes Related to Geldanamycin Biosynthesis in Streptomyces hygroscopicus 17997
He, Weiqing; Wu, Linzhuan; Gao, Qunjie; Du, Yu; Wang, Yiguang
/Amr Vector with apramycin resistant gene This ...
... were cultured at 37°C in LB medium. Plasmids  pUC18 
Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T
Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T
Zhao, Baisuo; Lu, Weidong; Yang, Lifu; Zhang, Bo; Wang, Lei; Yang, Su Sheng
and pGEM T-easy vector were used in cloning and ...
... the gene cloning experiments. Cloning vectors  pUC18 
Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans
Gene cloning and in vivo characterization of a dibenzothiophene dioxygenase from Xanthobacter polyaromaticivorans
Hirano, Shin-ichi; Haruki, Mitsuru; Takano, Kazufumi; Imanaka, Tadayuki; Morikawa, Masaaki; Kanaya, Shigenori
, pT7Blue-T, and pSTV28 were purchased from ...
... 204 chromosomal DNA, subcloned into  pUC18 
Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system
Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system
Mahfouz, Magdy E; Grayson, T Hilton; Dance, David AB; Gilpin, Martyn L
, sequenced on both strands to confirm the ...
... transformation and expression host. The plasmid  pUC18 
Cloning, purification, and characterization of chitosanase from Bacillus sp. DAU101
Cloning, purification, and characterization of chitosanase from Bacillus sp. DAU101
Lee, Y. S.; Yoo, J. S.; Chung, S. Y.; Lee, Y. C.; Cho, Y. S.; Choi, Y. L.
was used as a cloning vector and pGEX-6P-1 ...
... the genome, between the L1 ORF and the LCR. The  puc18 
Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Angeletti, Peter C.
backbone can be excised by a BamHI digest. ...
... BamHI/ NdeI fragment of pPIG4 ligated into  pUC18 
Disruption of the copper efflux pump (CopA) of Serratia marcescens ATCC 274 pleiotropically affects copper sensitivity and production of the tripyrrole secondary metabolite, prodigiosin
Disruption of the copper efflux pump (CopA) of Serratia marcescens ATCC 274 pleiotropically affects copper sensitivity and production of the tripyrrole secondary metabolite, prodigiosin
Williamson, N. R.; Simonsen, H. T.; Harris, A. K. P.; Leeper, F. J.; Salmond, George P. C.
This study pNRW31 SphI digested Cm marker ...
... pBeloBAC11 Cmr, BAC cloning vector Invitrogen  pUC18 
Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars
Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars
Genka, Hiroyuki; Baba, Tomoya; Tsuda, Masataka; Kanaya, Shigehiko; Mori, Hirotada; Yoshida, Takanobu; Noguchi, Masako Tsujimoto; Tsuchiya, Kenichi; Sawada, Hiroyuki
Apr, cloning vector TaKaRa pUC4K Apr Kmr, ...
... receptor-associated factor 6 TRAF6 3.49 0.56  PUC18 
Induction of tumour necrosis factor receptor-expressing macrophages by interleukin-10 and macrophage colony-stimulating factor in rheumatoid arthritis
Induction of tumour necrosis factor receptor-expressing macrophages by interleukin-10 and macrophage colony-stimulating factor in rheumatoid arthritis
Takasugi, Koji; Yamamura, Masahiro; Iwahashi, Mitsuhiro; Otsuka, Fumio; Yamana, Jiro; Sunahori, Katsue; Kawashima, Masanori; Yamada, Masao; Makino, Hirofumi
...
... ATCC 33679 Johnson et al. 1980 Plasmids    pUC18 
New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics
New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics
Moldes, Cristina; Farinós, Gema P.; Eugenio, Laura I.; García, Pedro; García, José L.; Ortego, Félix; Hernández-Crespo, Pedro; Castañera, Pedro; Prieto, María A.
Apr, cloning vector Sambrook and Russell 2001 ...
... were used for expression experiments. Plasmids  pUC18 
A gene linB2 responsible for the conversion of β-HCH and 2,3,4,5,6-pentachlorocyclohexanol in Sphingomonas sp. BHC-A
A gene linB2 responsible for the conversion of β-HCH and 2,3,4,5,6-pentachlorocyclohexanol in Sphingomonas sp. BHC-A
Wu, Jun; Hong, Qing; Han, Peng; He, Jian; Li, Shunpeng
(Ampr) and pET29a (Kmr) were used for cloning ...
... proA + B + lacI a lacZΔM15] [30] Plasmids  pUC18 
Cloning, Sequencing, and Characterization of the Azospirillum brasilense fhuE Gene
Cloning, Sequencing, and Characterization of the Azospirillum brasilense fhuE Gene
Cui, Yanhua; Tu, Ran; Guan, Yue; Ma, Luyan; Chen, Sanfeng
Cloning vector, Apr [40] pVK100 Cloning vector, ...
... pCR2.1 [10] and subsequently subcloned into  pUC18 
Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients
Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients
Burns, Malcolm; Corbisier, Philippe; Wiseman, Gordon; Valdivia, Hernan; McDonald, Paul; Bowler, Peter; Ohara, Katrina; Schimmel, Heinz; Charels, Diana; Damant, Andrew; Harris, Neil
to yield pIRMM-0030 and pIRMM-0031, ...
... with adhA gene from strain TKN14 This study    pUC18 
Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols
Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols
Peng, Xue; Taki, Hironori; Komukai, Syoko; Sekine, Mitsuo; Kanoh, Kaneo; Kasai, Hiroaki; Choi, Seon-Kang; Omata, Seiha; Tanikawa, Satoshi; Harayama, Shigeaki; Misawa, Norihiko
Apr, cloning vector Yanisch-Perron et al. 1985 ...