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... fragments of 1,769 and 676 bp from  pUC18 
Genome-specific primer sets for starch biosynthesis genes in wheat
Genome-specific primer sets for starch biosynthesis genes in wheat
Blake, N. K.; Sherman, J. D.; Dvořák, J.; Talbert, L. E.
digested with RsaI. ...
... along with chrysotile asbestos fibers and  pUC18 
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Yoshida, N.; Saeki, Y.
donor DNA, was spread over the surface of a ...
... different plasmids were used in this study: the  pUC18 
Obtaining transgenic plants using the bio-active beads method
Obtaining transgenic plants using the bio-active beads method
Liu, Haibo; Kawabe, Akira; Matsunaga, Sachihiro; Murakawa, Tomoko; Mizukami, Atsushi; Yanagisawa, Masanobu; Nagamori, Eiji; Harashima, Satoshi; Kobayashi, Akio; Fukui, Kiichi
-sGFP (4.1-kb) plasmid containing a cauliflower ...
... subcloned into a double-stranded vector such as  pUC18 
BAC Finishing Strategies
BAC Finishing Strategies
Bird, Christine; Grafham, Darren
or single-stranded M13 vector. ...
... cloning of PCR products (Promega, Madison, WI);  pUC18 
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Zoulim, Fabien; Tong, ShuPing; Trépo, Christian
or pUC19 for whole HBV-genome cloning (New ...
... For example, if the BAC library was made in  pUC18 
Transposon-Mediated Sequencing
Transposon-Mediated Sequencing
Reeg, Rachel; Madan, Anup
( Fig. 2 ), cloned into the SmaI site, it would ...
... HI and subcloned into the Bam HI/Sma I sites of  pUC18 
Genomic organization of the Neurospora crassa gsn gene: possible involvement of the STRE and HSE elements in the modulation of transcription during heat shock
Genomic organization of the Neurospora crassa gsn gene: possible involvement of the STRE and HSE elements in the modulation of transcription during heat shock
Zanolli Freitas, F.; Bertolini, M. C.
, generating the plasmid pUC-HSE. ...
... The C427-encoding gene was subcloned into a  pUC18 
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido)cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain
Nagao, Koji; Yamashita, Michio; Ueda, Mitsuyoshi
vector. pYS9107K, a plasmid for C427 expression ...
... in acetate minimal medium This work Plasmids    pUC18 
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis
Netzer, Roman; Krause, Malgorzata; Rittmann, Doris; Peters-Wendisch, Petra G.; Eggeling, Lothar; Wendisch, Volker F.; Sahm, Hermann
Cloning vector; Apr Norrander et al. (1983) ...
... (Aldrich). 19. Core streptavidin gene in  pUC18 
Smart Polymer-Streptavidin Conjugates
Smart Polymer-Streptavidin Conjugates
Stayton, Patrick S.; Ding, Zhongli; Hoffman, Allan S.
plasmid. ...
... recA∷Rp-2 Tc∷Mu Simon et al. (1983) Plasmids    pUC18 
Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs
Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs
Brichta, Dayna M.; Azad, Kamran N.; Ralli, Pooja; O’Donovan, Gerard A.
/19 Apr cloning vectors, source of lacZα ...
... produces an intracellular DNA plasmid product (  pUC18 
Optimal operation of high-pressure homogenization for intracellular product recovery
Optimal operation of high-pressure homogenization for intracellular product recovery
Kelly, William J.; Muske, Kenneth R.
). ...
... RV PHA-/Hup- double mutant This work Plasmids  pUC18 
Metabolically Engineered Rhodobacter sphaeroides RV strains for Improved Biohydrogen Photoproduction Combined with Disposal of Food Wastes
Metabolically Engineered Rhodobacter sphaeroides RV strains for Improved Biohydrogen Photoproduction Combined with Disposal of Food Wastes
Franchi, Elisabetta; Tosi, Claudio; Scolla, Giuseppe; Penna, Gino Della; Rodriguez, Francesco; Pedroni, Paola Maria
Apr, lacZa Yanisch-Perron et al. (1985) ...
... pCR2.1 (Invitrogen, Carlsbad, Calif., USA);  pUC18 
Cloning of l-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485
Cloning of l-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485
Desai, S. G.; Guerinot, M. L.; Lynd, L. R.
(Invitrogen); pBLUESCRIPT II SK(+) ...
... The PCR fragments were subcloned into a SmaI  pUC18 
Cloning, expression and partial characterization of a gene encoding the S15a ribosomal protein of Taenia solium
Cloning, expression and partial characterization of a gene encoding the S15a ribosomal protein of Taenia solium
Jiménez, Lucía; Becerra, Arturo; Landa, Abraham
vector via blunt-end ligation, sequenced as ...
... WAVE Nucleic Acid System. WAVE traces of: (A) a  pUC18 
Mutation Scanning for the Clinical Laboratory
Mutation Scanning for the Clinical Laboratory
Harvey, John F.; Sampson, Julian R.
HaeIII digest, (B) a sample heterozygous for an ...
... fragments were inserted into a modified  pUC18 
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Horstmann, Verena; Huether, Claudia M; Jost, Wolfgang; Reski, Ralf; Decker, Eva L
vector, where every construct contained the ...
... 10 mM rATP, pH 7.5 in sterile H2O (4X 250 mL);  pUC18 
Development of a Mammalian Tet-On Expression Cell Line
Development of a Mammalian Tet-On Expression Cell Line
Liu, Yong-Yu; Cabot, Myles C.
plasmid control DNA, BamHI digested (10mg), T4 ...
... (Technical University of Gdansk, Poland).  pUC18 
Heterologous gene expression in Thermus thermophilus: β-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase
Heterologous gene expression in Thermus thermophilus: β-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase
Park, Ho-Shin; Kayser, Kevin J.; Kwak, Jung-Ho; Kilbane, John J., II
was purchased from New England Biolabs ...
... pGEM3zF+lacZ′-dnaK Vh AmpR This work  pMZ40  pUC18 
Complementation studies of the DnaK–DnaJ–GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro
Complementation studies of the DnaK–DnaJ–GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro
Żmijewski, Michał A.; Kwiatkowska, Joanna M.; Lipińska, Barbara
-grpE Vh AmpR This work  pBR322 AmpR, TetR Our ...
... kgmB gene. The kgmB gene was first cloned into  pUC18 
The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated
The kgmB gene, encoding ribosomal RNA methylase from Streptomyces tenebrarius, is autogenously regulated
Vajic, Sandra; Anastasov, Natasa; Vasiljevic, Branka
to yield pUΔK1. ...
... Complete angE cloned in pMD18 This work    pUC18 
Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1
Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1
Liu, Qin; Ma, Yue; Wu, Haizhen; Shao, Mingfei; Liu, Haifeng; Zhang, Yuanxing
Cloning/expression vector Sangon (Shanghai, ...
... was fused to the 3′-terminal end of GFP in the  PUC18 
A salt-responsive receptor-like kinase gene regulated by the ethylene signaling pathway encodes a plasma membrane serine/threonine kinase
A salt-responsive receptor-like kinase gene regulated by the ethylene signaling pathway encodes a plasma membrane serine/threonine kinase
He, X.-J.; Zhang, Z.-G.; Yan, D.-Q.; Zhang, J.-S.; Chen, S.-Y.
vector, which can be expressed under the ...
... was digested with HindIII and subcloned into  pUC18 
Chemolithoautotrophy in the marine, magnetotactic bacterial strains MV-1 and MV-2
Chemolithoautotrophy in the marine, magnetotactic bacterial strains MV-1 and MV-2
Bazylinski, Dennis A.; Dean, Annette J.; Williams, Timothy J.; Long, Linda Kimble; Middleton, Shawna L.; Dubbels, Bradley L.
following standard ligation and transformation ...
... dehydrogenase. Materials and methods Materials  pUC18 
Gene and primary structures of dye-linked l-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
Gene and primary structures of dye-linked l-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
Kawakami, Ryushi; Sakuraba, Haruhiko; Ohshima, Toshihisa
, pET15b, and TKR MEGALABEL were purchased from ...