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... RRS (IRMM-410R), and from the cloning vector  pUC18 
Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays)
Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays)
Rønning, Sissel B.; Vaïtilingom, Marc; Berdal, Knut G.; Holst-Jensen, Arne
(Amersham Biotech) were used as templates in ...
... intergenic region (Fig. 1A) was cloned into  pUC18 
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Samsonova, Natalya N; Smirnov, Sergey V; Altman, Irina B; Ptitsyn, Leonid R
vector and sequenced. ...
... imm downstream of the tet R promoter.  pUC18 
Stationary phase-like properties of the bacteriophage λ Rex exclusion phenotype
Stationary phase-like properties of the bacteriophage λ Rex exclusion phenotype
Slavcev, R. A.; Hayes, S.
and pUC19 were obtained from New England ...
... below). Controls Nick-translated biotin-labeled  pUC18 
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Evans, Mark F; Aliesky, Holly A; Cooper, Kumarasen
plasmid was used as a control for non-specific ...
... processes were evaluated with commercial  pUC18 
DNA cleavage studies of mononuclear and dinuclear copper(II) complexes with benzothiazolesulfonamide ligands
DNA cleavage studies of mononuclear and dinuclear copper(II) complexes with benzothiazolesulfonamide ligands
González-Álvarez, Marta; Alzuet, Gloria; Borrás, Joaquín; Pitié, Marguerite; Meunier, Bernard
(Roche Diagnostics, Germany) (0.25 µg/μL in ...
... from Promega (Madison, WI). 6. Ready-to-go  pUC18 
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Manley, Darren; Neil, Joe D.
, pre-cut at the SmaI site, is from Pharmacia ...
... was cloned into Sma I-cut, phosphatase-treated  pUC18 
Active-site residue, domain and module swaps in modular polyketide synthases
Active-site residue, domain and module swaps in modular polyketide synthases
Vecchio, Francesca; Petkovic, Hrvoje; Kendrew, Steven G.; Low, Lindsey; Wilkinson, Barrie; Lill, Rachel; Cortés, Jesús; Rudd, Brian A. M.; Staunton, Jim; Leadlay, Peter F.
, and demethylated plasmid DNA was produced by ...
... This work pLysE cam R T7 lysozyme F.W. Studier  pUC18 
Mutations arise independently of transcription in non-dividing bacteria
Mutations arise independently of transcription in non-dividing bacteria
Barionovi, D.; Ghelardini, P.; Lallo, G. Di; Paolozzi, L.
amp R Laboratory collection ...
... chromosome, Smr Simon et al. (1983) Plasmids    PUC18 
Poly β-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant
Poly β-hydroxybutyrate depolymerase (PhaZ) in Azospirillum brasilense and characterization of a phaZ mutant
Kadouri, Daniel; Jurkevitch, Edouard; Okon, Yaacov
Apr, ColE1 replicon, lacZ, cloning vector ...
... a bar expression cassette subcloned into  pUC18 
Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker
Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker
Ma, Biao; Mayfield, Mary B.; Gold, Michael H.
(New England Biolabs), which included, from 5′ ...
... fluorescent protein (GFP) reporter gene in  pUC18 
A rice transcription factor OsbHLH1 is involved in cold stress response
A rice transcription factor OsbHLH1 is involved in cold stress response
Wang, Yu-Jun; Zhang, Zhi-Gang; He, Xin-Jian; Zhou, Hua-Lin; Wen, Yu-Xiang; Dai, Ji-Xun; Zhang, Jin-Song; Chen, Shou-Yi
and sequenced. ...
... cloned into an EcoRI-HindIII site of modified  pUC18 
Random Insertion and Deletion Mutagenesis
Random Insertion and Deletion Mutagenesis
Murakami, Hiroshi; Hohsaka, Takahiro; Sisido, Masahiko
(pUM). ...
... cDNAs, which were cloned into the EcoRI site of  pUC18 
Cloning of a nitrate reductase inactivator (NRI) cDNA from Spinacia oleracea L. and expression of mRNA and protein of NRI in cultured spinach cells
Cloning of a nitrate reductase inactivator (NRI) cDNA from Spinacia oleracea L. and expression of mRNA and protein of NRI in cultured spinach cells
Sonoda, Masatoshi; Ide, Hiroaki; Nakayama, Shinya; Sasaki, Asako; Kitazaki, Shinei; Sato, Takahide; Nakagawa, Hiroki
. ...
... and cloned into the EcoRI site of plasmid  pUC18 
cAMP signaling in Aspergillus fumigatus is involved in the regulation of the virulence gene pksP and in defense against killing by macrophages
cAMP signaling in Aspergillus fumigatus is involved in the regulation of the virulence gene pksP and in defense against killing by macrophages
Liebmann, B.; Gattung, S.; Jahn, B.; Brakhage, A. A.
, resulting in plasmid pUCacyA1. ...
... PCR1 and PCR2, were amplified and cloned in  pUC18 
Development of cloning vectors and transformation methods for Amycolatopsis
Development of cloning vectors and transformation methods for Amycolatopsis
Dhingra, Gauri; Kumari, Rekha; Bala, Shashi; Majumdar, Swati; Malhotra, Shweta; Sharma, Poonam; Lal, Sukanya; Cullum, John; Lal, Rup
/19 (pAT8A and pAT8B respectively). ...
... the −1,076/−73 promoter region cloned in  pUC18 
Analysis of regulatory elements of the promoter and the 3′ untranslated region of the maize Hrgp gene coding for a cell wall protein
Analysis of regulatory elements of the promoter and the 3′ untranslated region of the maize Hrgp gene coding for a cell wall protein
Menossi, M.; Rabaneda, F.; Puigdomènech, P.; Martínez-Izquierdo, J. A.
). ...
... enzyme sites, and cloned into plasmid  pUC18 
The stem-loop region of the tobacco psbA 5′UTR is an important determinant of mRNA stability and translation efficiency
The stem-loop region of the tobacco psbA 5′UTR is an important determinant of mRNA stability and translation efficiency
Zou, Z.; Eibl, C.; Koop, H.-U.
by blunt-end ligation. ...
... colonies when “standard” (i.e., 107/μg of  pUC18 
Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)
Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)
Miyazaki, Kentaro
) competent cells are used. ...
... were digested with EcoRⅠ and HindⅢ, ligated in  pUC18 
Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid
Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid
Vidal, Ana M.; Ben-Cheikh, Waddi; Talón, Manuel; García-Martínez, José L.
and used to transform DH-5α-competent cells. ...
... Tetr gal (DE3) endA Hte Invitrogen Plasmids    pUC18 
A zinc-containing mannitol-2-dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291: purification of the enzyme and cloning of the gene
A zinc-containing mannitol-2-dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291: purification of the enzyme and cloning of the gene
Hahn, Gerald; Kaup, Björn; Bringer-Meyer, Stephanie; Sahm, Hermann
, pUC19 2.7 kb ; bla, lacZα Vieira and Messing ...
... was constructed by cloning DNA fragments into  pUC18 
Cloning, sequence analysis, and heterologous expression of the gene encoding a (S)-specific alcohol dehydrogenase from Rhodococcus erythropolis DSM 43297
Cloning, sequence analysis, and heterologous expression of the gene encoding a (S)-specific alcohol dehydrogenase from Rhodococcus erythropolis DSM 43297
Abokitse, K.; Hummel, W.
. ...
... into the EcoRI/Bam HI and BamHI/SalI sites of  PUC18 
Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-α Moiety
Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-α Moiety
Xiang, Jim; Gordon, John R.
vector to form the ...
... (Rockville, MD). The HaeIIIdigest of  pUC18 
Denaturing High-Performance Liquid Chromatography
Denaturing High-Performance Liquid Chromatography
Premstaller, Andreas; Oefner, Peter J.
is from Sigma (St. ...
... DNA were ligated into a SmaI/EcoRI-digested  pUC18 
Analysis of the structure and inheritance of a linear plasmid from the obligate biotrophic fungus Blumeria graminis f. sp. hordei
Analysis of the structure and inheritance of a linear plasmid from the obligate biotrophic fungus Blumeria graminis f. sp. hordei
Giese, H.; Lyngkjaer, M. F.; Stummann, B. M.; Grell, M. N.; Christiansen, S. K.
vector according to standard procedures ...
... using pJM102, which is a derivative of  pUC18 
Genetic system constructed to overproduce and secrete proinsulin in Bacillus subtilis
Genetic system constructed to overproduce and secrete proinsulin in Bacillus subtilis
Olmos-Soto, J.; Contreras-Flores, R.
and carries a cat gene inserted in NdeI ...