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... Preparation of Plasmid Vector 1. Plasmid  pUC18 
Monitoring Drug Resistance for Herpesviruses
Monitoring Drug Resistance for Herpesviruses
Andrei, Graciela; Fiten, Pierre; Clercq, Erik; Snoeck, Robert; Opdenakker, Ghislain
(Pharmacia Biotech). 2. BamHI restriction ...
... active β-galactosidase has been synthesized. 2.  pUC18 
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
Braman, Jeffrey; Papworth, Carol; Greener, Alan
Control plasmid (0.1 ng/µL) was also obtained ...
... “TE only” negative control transformation and a  pUC18 
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
Lail-Trecker, Melissa
positive control transformation (supplied with ...
... entire plasmid pJK2-1 and the entire plasmid  pUC18 
Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-1 and application in the development of a cloning vector
Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-1 and application in the development of a cloning vector
Trček, J.; Raspor, P.; Teuber, M.
was successfully used in transformation ...
... IGF-I was kindly provided by Martin Jansen (5).  pUC18 
Antisense IGF-I for Hepatocellular Carcinoma
Antisense IGF-I for Hepatocellular Carcinoma
Liu, Yanjun; Zhao, Jian; Lu, Yang; Trojan, Jerzy; Wu, Mengchao; Guo, Yajun
was kindly provided by Peter Harte (4).REP-4 ...
... containing the replication origin was cloned in  pUC18 
A Replication Origin of Bacillus thuringiensis
A Replication Origin of Bacillus thuringiensis
Yu, Jianxiu; Zhang, Yingjie; Pang, Yi; Xu, Minqing
and sequenced. ...
... a PstI rice (var. Malkolam) genomic library in  pUC18 
Sequence variations at a complex microsatellite locus in rice and its conservation in cereals
Sequence variations at a complex microsatellite locus in rice and its conservation in cereals
Davierwala, A. P.; Ramakrishna, W.; Ranjekar, P. K.; Gupta, V. S.
. ...
... of the creatininase gene, was subcloned into a  pUC18 
Expression of the creatininase gene from Pseudomonas putidaRS65 in Escherichia coli
Expression of the creatininase gene from Pseudomonas putidaRS65 in Escherichia coli
Tang, T-Y; Wen, C-J; Liu, W-H
vector and the nucleotide sequence of the ...
... of Chinese bovine Sry gene were subcloned into  pUC18 
Structural characterization of the Sry 5’ regulatory region of bovine
Structural characterization of the Sry 5’ regulatory region of bovine
Hui-fang, Shi; Han-hua, Cheng; Yi-qing, Guo; Li, Liu; Qi-xing, Yu; Rong-jia, Zhou
. ...
... of replication of the high copy-number plasmid  pUC18 
Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES
Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES
Heinrich, Jochen; Strack, Bettina; Nawrath, Michael; Pavlovic, Jovan; Moelling, Karin
in bacteria allows the production of large ...
... efficiencies of 2−6 × 1010 CFU/µg supercoiled  pUC18 
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
Engberg, Jan; Johansen, Lene K.; Westengaard-Hildinge, Michelle; Riise, Erik S.; Albrechtsen, Bjarne
test DNA (Stratagene) with ToplO/F′ and TG1 ...
... the Plasmid Vector 1. The plasmid vector (e.g.,  pUC18 
Identification of Differentially Expressed Genes in Young and Senescent T Cells
Identification of Differentially Expressed Genes in Young and Senescent T Cells
Engel, Andrea; Adibzadeh, Mahdi; Pawelec, Graham
, pUC19, Bluescript, or any other vector) is ...
... and 5′ untranslated sequences in a  pUC18 
Development and Characterization of Lyophilized DNA Vaccine Formulations
Development and Characterization of Lyophilized DNA Vaccine Formulations
Shen, Nancy L.; Hartikka, Jukka; Horn, Nancy A.; Manthorpe, Marston; Marquet, Magda
backbone. 3. Plasmid VR1223 (19,20) encodes the ...
... fragment of the human α2C-AR gene cloned into  pUC18 
Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors
Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors
Schaak, Stéphane; Devedjian, Jean-Christophe; Paris, Hervé
(24). ...
... vector such as Bluescript II (Stratagene) or  pUC18 
Isolation of Adrenergic Receptor Genes
Isolation of Adrenergic Receptor Genes
Scofield, Margaret A.; Deupree, Jean D.; Bylund, David B.
/19 (Life Technologies). 2. Both strands of the ...