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... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... together with the T7 promoter into plasmid  pUC18 
In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons
In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons
Kramer, Gisela; Kudlicki, Wieslaw; Hardesty, Boyd
followed by transcription of the DNA is ...
... Plasmid DNA∶ pCV17 (5), pTrans, pROK2, pROK8,  pUC18 
Manipulation of Photosynthetic Metabolism
Manipulation of Photosynthetic Metabolism
Parry, Martin A. J.; Colliver, Steven P.; Madgwick, Pippa J.; Paul, Matthew J.
(9). 51. Prehybridization solution∶ 0.5M ...
... bacterium,Cellulomonas biazotea, were cloned in  pUC18 
Cloning and expression of β-glucosidase genes inEscherichia coli andSaccharomyces cerevisiae using shuttle vector pYES 2.0
Cloning and expression of β-glucosidase genes inEscherichia coli andSaccharomyces cerevisiae using shuttle vector pYES 2.0
Rajoka, M. I.; Bashir, A.; Hussain, S. R. A.; Ghauri, M. T.; Parvez, R.; Malik, K. A.
in itsSacI cloning site and transformed toE. ...
... (13). The resultant plasmid contains the  pUC18 
Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
Pérez, Esther; Gavigan, Julie-Ann; Otal, Isabel; Guilhot, Christophe; Pelicic, Vladimir; Giquel, Brigitte; Martin, Carlos
replicon for replication in E. coli and the ...
... amplified cDNA (766 bp) into the SmaI site of  pUC18 
I1PP2A and I2PP2A
I1PP2A and I2PP2A
Li, Mei; Damuni, Zahi
in LR buffer, and then transform Escherichia ...
... six restriction fragments were sub cloned into  pUC18 
Subcloning and Sequencing of DNA Fragment Related to Salt Tolerance in Sinorhizobium fredii
Subcloning and Sequencing of DNA Fragment Related to Salt Tolerance in Sinorhizobium fredii
Yang, S. S.; Bian, X. L.; Sun, H. H.; Gao, W. M.
for sequencing. ...
... the polymerase chain reaction, subcloned into  pUC18 
Partial sequencing of Reissner’s fiber glycoprotein I (RF-Gly I)
Partial sequencing of Reissner’s fiber glycoprotein I (RF-Gly I)
Nualart, F.; Hein, S.; Yulis, C. R.; Zárraga, A. M.; Araya, A.; Rodríguez, E. M.
vector, and sequenced. ...
... EcoRI site within the lacZ gene of the plasmid  pUC18 
A rapid method to monitor repair and mis-repair of DNA double-strand breaks by using cell extracts of the yeast Saccharomyces cerevisiae
A rapid method to monitor repair and mis-repair of DNA double-strand breaks by using cell extracts of the yeast Saccharomyces cerevisiae
Jha, Bhavanath; Ahne, F.; Kistler, Manfred; Klaus, Christian; Eckardt-Schupp, Friederike
and the plasmid was exposed to cellular ...
... aac(6′)-Ill L29044 pAT479 ~ 2300 Hindlll(  pUC18 
The Application of Molecular Techniques for the Study of Aminoglycoside Resistance
The Application of Molecular Techniques for the Study of Aminoglycoside Resistance
Shaw, Karen J.; Sabatelli, Frank J.; Naples, Linda; Mann, Paul; Hare, Roberta S.; Miller, George H.
) 172 (Sspl/Hlndlll) 400 (HL/HR) (14, 15) ...
... marinus, strain ITI 378, was constructed in  pUC18 
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
Halldórsdóttir, S.; Thórólfsdóttir, E. T.; Spilliaert, R.; Johansson, M.; Thorbjarnardóttir, S. H.; Palsdottir, A.; Hreggvidsson, G. Ó.; Kristjánsson, J. K.; Holst, O.; Eggertsson, G.
and transformed into Escherichia coli. ...
... cloning chromosomal DNA of mutants into  pUC18 
Tn917 transposon mutagenesis and marker rescue of interrupted genes of Streptococcus mutans
Tn917 transposon mutagenesis and marker rescue of interrupted genes of Streptococcus mutans
Cvitkovitch, Dennis G.; Gutierrez, Juan A.; Crowley, Paula J.; Wojciechowski, Laura; Hillman, Jeffrey D.; Bleiweis, Arnold S.
, followed by selection of ampicillin and ...
... cloning chromosomal DNA of mutants into  pUC18 
Tn917 transposon mutagenesis and marker rescue of interrupted genes of Streptococcus mutans
Tn917 transposon mutagenesis and marker rescue of interrupted genes of Streptococcus mutans
Cvitkovitch, Dennis G.; Gutierrez, Juan A.; Crowley, Paula J.; Wojciechowski, Laura; Hillman, Jeffrey D.; Bleiweis, Arnold S.
, followed by selection of ampicillin and ...
... This fragment was drrectronally ligated mto  pUC18 
Photorhabdus luminescens luxCDABE Promoter Probe Vectors
Photorhabdus luminescens luxCDABE Promoter Probe Vectors
Dyk, Tina K.; Rosson, Reinhardt A.
DNA that had been digested with EcoRI and SstI ...
... co1i that do not replicate in H ducreyi, (e.g.,  pUC18 
Chancroid
Chancroid
Morse, Stephen A.; Trees, David L.; Totten, Patricia A.
) and a portion of the gene is deleted and ...
... is inserted into the HindIII-BamHI site of a  pUC18 
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
Brierley, Russell A.
-based vector directly downstream of the DNA ...
... to at least 1 x 108 colonies/pg supercoiled  pUC18 
Differential Display to Define Molecular Markers and Genes That Mediate Malignancy
Differential Display to Define Molecular Markers and Genes That Mediate Malignancy
Pavlik, Edward J.; Nelson, Katherine; Srinivasan, Suseela; Johnson, Thomas L.; Priest, Paul D.
DNA 2. ...
... terminator cassette present in  pUC18 
Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Trafficking and Stability of Heterologous Proteins in Transgenic Plants
Napier, Johnathan A.; Richard, Gaelle; Shewry, Peter R.
) suitable for direct subcloning into a ...
... gel. 4. Ligate into EcoRI and HindIII-cleaved  pUC18 
Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana
Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana
Pujol, Gemma; Ferrer, Albert; Ariño, Joaquin
or equivalent vector Sequence the insert to ...