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... in the catalog. If, for example, you have  pUC18 
SEQMAN
SEQMAN
Swindell, Simon R.; Plasterer, Thomas N.
cut with SmaI, defined as a vector and want to ...
... 6. PCR oligonucleotide primers. 7. Plasmid  pUC18 
Molecular Characterization of H. pylori Surface Antigens
Molecular Characterization of H. pylori Surface Antigens
Doig, Peter; O’Toole, Paul W.; Trust, Trevor J.
. 8. PCR reaction buffer. 10 mM Tris-HCl, pH ...
... “TE only” negative control transformation and a  pUC18 
Cloning PCR Products Utilizing the T/A Overhang and a Kit
Cloning PCR Products Utilizing the T/A Overhang and a Kit
Lail-Trecker, Melissa
positive control transformation (supplied with ...
... purchased directly from Pharmacia (Ready-To-Go  pUC18 
Using T4 DNA Polymerase to Generate Clonable PCR Products
Using T4 DNA Polymerase to Generate Clonable PCR Products
Wang, Kai
cloning kit). 9. In the ligation reaction, we ...
... amplification of a 4.8-kb plasmid derived from  pUC18 
GC-Rich Template Amplification by Inverse PCR
GC-Rich Template Amplification by Inverse PCR
Moreau, Alain; Duez, Colette; Dusart, Jean
and containing a 1980-bp insert, the gene ...
... plasmid, p1/1. The genes were subcloned into  pUC18 
Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida
Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida
Wahbi, L.P.; Phumathon, P.; Brown, A.; Minter, S.; Stephens, G.M.
and pKK223-3 and expressed under the lac and ...