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... active β-galactosidase has been synthesized. 2.  pUC18 
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
Braman, Jeffrey; Papworth, Carol; Greener, Alan
control plasmid (0.1 ng/µL) was also obtained ...
... whereas, M90T containing the control vector,  pUC18 
ThenadB gene ofSalmonella typhimurium complements the nicotinic acid auxotrophy ofShigella flexneri
ThenadB gene ofSalmonella typhimurium complements the nicotinic acid auxotrophy ofShigella flexneri
Mantis, N. J.; Sansonetti, P. J.
does not, suggesting that this species ...
... and its flanking sequences were cloned into  pUC18 
Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenases
Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenases
Synstad, Bjørnar; Emmerhoff, Oddmund; Sirevåg, R.
/19, indicating that the putative σ70 promoter ...
... coli BL21[DE3] (14), vectors PET-3a (14), and  pUC18 
Homolog Scanning
Homolog Scanning
Wang, Lin-Fa
(15). 4. Oligonucleotide primers. a. Flanking ...
... Fig. 2 (from ref. 9). The plasmid is based on a  pUC18 
Random Fragment Libraries Using Yeast Expression Plasmid
Random Fragment Libraries Using Yeast Expression Plasmid
Bénichou, Serge; Inchauspé, Geneviève
backbone, and is able to replicate in both E. ...
... andDecapterus were subcloned in theSmaI site of  pUC18 
PCR amplification of cDNAs of fish hemoglobin β chains using a consensus primer: cDNA-derived amino acid sequences of β chains from the catfishParasilurus asotus and the scadDecapterus maruadsi
PCR amplification of cDNAs of fish hemoglobin β chains using a consensus primer: cDNA-derived amino acid sequences of β chains from the catfishParasilurus asotus and the scadDecapterus maruadsi
Suzuki, Tomohiko; Nishikawa, Teppei
and cDNA-derived amino acid sequences of 147 ...
... dilution of a supercoiled plasmid, such as  pUC18 
Transformation of Bacteria by Electroporation
Transformation of Bacteria by Electroporation
Drury, Lucy
, to use as a positive control in all ...
... in Escherichia coli using the expression vector  pUC18 
Molecular cloning and expression of a Micromonospora chalcae β-glucosidase encoding gene in Escherichia coli
Molecular cloning and expression of a Micromonospora chalcae β-glucosidase encoding gene in Escherichia coli
Winters, A.; Gallagher, J.; Barron, N.; Rollan, A.; McHale, A. P.
. ...
... cells using a routine plasmid vector, such as  pUC18 
Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
Hales, Barbara Anne; Winstanley, Craig
/19. ...
... a CsCl/actinomycin-D gradient and cloned into  pUC18 
Cloning and analysis of a 6.8-kb rDNA intergenic spacer region of the European ash (Fraxinus excelsior L.)
Cloning and analysis of a 6.8-kb rDNA intergenic spacer region of the European ash (Fraxinus excelsior L.)
Jeandroz, S.; Pugin, A.; Bervillé, A.
for further characterization. ...
... was obtained, which was then subcloned in  pUC18 
Characterization of γ-crystallin from the eye lens of bullfrog: Complexity of γ-crystallin multigene family as revealed by sequence comparison among different amphibian species
Characterization of γ-crystallin from the eye lens of bullfrog: Complexity of γ-crystallin multigene family as revealed by sequence comparison among different amphibian species
Lu, Shao-Fan; Pan, Fu-Ming; Chiou, Shyh-Horng
vector and transformed intoEscherichia coli ...
... dGTP. 4. Forward and reverse PCR primers for  pUC18 
The Use of Selective Enrichment for the Isolation of Species-Specific DNA Probes for Insects
The Use of Selective Enrichment for the Isolation of Species-Specific DNA Probes for Insects
Clapp, Justin P.
: 5′CACACAGGAAACAGCTATG and ...
... EcoRI SacI KpnI SmaI BamHI XbaI portion of the  pUC18 
An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
Sedgwick, Steven G.; Morgan, Brian A.; Man, Nguyen thi; Morris, Glenn E.
polylinker. ...
... and 10mM 7dc-dGTP. 3. Some preparations of  pUC18 
Using the Automated DNA Sequencer
Using the Automated DNA Sequencer
Du, Zijin; Wilson, Richard K.
and pUC1 18 have deleted one nucleotide at the ...