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... Kit (Biobasic Inc.) and ligated into the  pUC18 
Development, characterization and utilization of GenBank microsatellite markers in Phyllostachys pubescens and related species
Development, characterization and utilization of GenBank microsatellite markers in Phyllostachys pubescens and related species
Tang, Ding-Qin; Lu, Jiang-Jie; Fang, Wei; Zhang, Shan; Zhou, Ming-Bing
vector (TaKaRa, Japan). ...
... pOCT Octane (component of crude oil) P. putida  pUC18 
Biodegradation and Bioremediation of Organic Chemical Pollutants by Pseudomonas
Biodegradation and Bioremediation of Organic Chemical Pollutants by Pseudomonas
Kahlon, Rachhpal S.
Dibenzothiophene naphthalene, phenanthrene P. ...
... restriction enzymes XbaI (site present in the  pUC18 
The Xerophyta viscosa Aldose Reductase (ALDRXV4) Confers Enhanced Drought and Salinity Tolerance to Transgenic Tobacco Plants by Scavenging Methylglyoxal and Reducing the Membrane Damage
The Xerophyta viscosa Aldose Reductase (ALDRXV4) Confers Enhanced Drought and Salinity Tolerance to Transgenic Tobacco Plants by Scavenging Methylglyoxal and Reducing the Membrane Damage
Kumar, Deepak; Singh, Preeti; Yusuf, Mohd Aslam; Upadhyaya, Chandrama Prakash; Roy, Suchandra Deb; Hohn, Thomas; Sarin, Neera Bhalla
MCS of pC2301) and BstEII (site present ...
... 1. The purified PCR product was subcloned into  pUC18 
Polymorphism in microRNA-binding site in HNF1B influences the susceptibility of type 2 diabetes mellitus: a population based case–control study
Polymorphism in microRNA-binding site in HNF1B influences the susceptibility of type 2 diabetes mellitus: a population based case–control study
Goda, Naoki; Murase, Haruna; Kasezawa, Nobuhiko; Goda, Toshinao; Yamakawa-Kobayashi, Kimiko
vector. ...
... 366, Zhou 8425B, Lumai 21 (lanes 1–18). M:  pUC18 
Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai
Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai
Xu, Hongxing; Yi, Yanjie; Ma, Pengtao; Qie, Yanmin; Fu, Xiaoyi; Xu, Yunfeng; Zhang, Xiaotian; An, Diaoguo
/MspI, numbers to the left are band sizes (bp), ...
... of this system by cloning it in the multicopy  pUC18 
Type II Toxin-Antitoxin Loci Encoded by Plasmids
Type II Toxin-Antitoxin Loci Encoded by Plasmids
Diago-Navarro, Elizabeth; Hernández-Arriaga, Ana M.; Díaz-Orejas, Ramón
plasmid leads to partial neutralization of the ...
... of which was under the CaMV 35S promoter in  pUC18 
Identification and Functional Analysis of the NADK Gene Family in Wheat
Identification and Functional Analysis of the NADK Gene Family in Wheat
Wang, Xiang; Li, Wen-Yan; Zhang, Mao-Mao; Gao, Yin-Tao; Liu, Wen-Ting; Li, Wen-Qiang; Muhammad, Izhar; Chen, Kun-Ming
vector, were transformed into rice ...
... bacterial alkaline phosphatase-treated  pUC18 
Satellite repeats in the functional centromere and pericentromeric heterochromatin of Medicago truncatula
Satellite repeats in the functional centromere and pericentromeric heterochromatin of Medicago truncatula
Kulikova, Olga; Geurts, René; Lamine, Monique; Kim, Dong-Jin; Cook, Douglas R.; Leunissen, Jack; Jong, Hans; Roe, Bruce A.; Bisseling, Ton
(Pharmacia), and Escherichia coli strain ...
... an Escherichia coli plasmid, such as  pUC18 
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis
Bifani, Pablo; Kurepina, Natalia; Mathema, Barun; Wang, Xiao-Ming; Kreiswirth, Barry
, containing the cloned 3′-IS6110 fragment as ...
... were ligated either into the BamHI site of  pUC18 
Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias
Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias
van der Walt, Eric; Martin, Darren P; Varsani, Arvind; Polston, Jane E; Rybicki, Edward P
using standard techniques (all clones labelled ...
... of a 311 bp fragment, which was cloned into the  pUC18 
Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881
Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881
Ryzhkov, Pavel M.; Ostermann, Kai; Rödel, Gerhard
vector and sequenced. ...
... B − ),gal(DE3) Novagen Plasmids  pUC18 
Identification of α-d-glucose-1-phosphate cytidylyltransferase involved in Ebosin biosynthesis of Streptomyces sp. 139
Identification of α-d-glucose-1-phosphate cytidylyltransferase involved in Ebosin biosynthesis of Streptomyces sp. 139
Qi, Xiao-Qiang; Sun, Qing-Li; Bai, Li-Ping; Shan, Jun-Jie; Zhang, Yang; Zhang, Ren; Li, Yuan
E. coli general cloning vector with multiple ...
... mechanically sheared DNA (2.5–5 Kb) into  pUC18 
Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
Legault, Boris A; Lopez-Lopez, Arantxa; Alba-Casado, Jose Carlos; Doolittle, W Ford; Bolhuis, Henk; Rodriguez-Valera, Francisco; Papke, R Thane
. ...
... was inserted into the PstI site of plasmid  pUC18 
Heterologous Production of Thermostable Proteins and Enzymes
Heterologous Production of Thermostable Proteins and Enzymes
Sakuraba, Haruhiko; Ohshima, Toshihisa
, which was then used to transform E. coli ...
... DNA fragment of 4,125 bp was ligated into  pUC18 
Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus
Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus
Sugareva, Venelina; Härtl, Albert; Brock, Matthias; Hübner, Katrin; Rohde, Manfred; Heinekamp, Thorsten; Brakhage, Axel A.
, also digested with EcoRI, yielding plasmid ...
... SSR fragment size ladders We initially used a  pUC18 
Robust and highly informative microsatellite-based genetic identity kit for potato
Robust and highly informative microsatellite-based genetic identity kit for potato
Ghislain, Marc; Núñez, Jorge; Rosario Herrera, María; Pignataro, José; Guzman, Frank; Bonierbale, Merideth; Spooner, David M.
sequencing reaction or a IRDye-50–350 size ...
... + (Kilaru et al. 2006) and p004iGM3 which is a  pUC18 
Paradoxical performance of tryptophan synthase gene trp1+ in transformations of the basidiomycete Coprinopsis cinerea
Paradoxical performance of tryptophan synthase gene trp1+ in transformations of the basidiomycete Coprinopsis cinerea
Dörnte, Bastian; Kües, Ursula
construct containing an enhanced gfp (egfp) ...
... amplified insert This study  pUCGmlox ApR, GmR,  pUC18 
Hydroxy-fatty acid production in a Pseudomonas aeruginosa 42A2 PHA synthase mutant generated by directed mutagenesis
Hydroxy-fatty acid production in a Pseudomonas aeruginosa 42A2 PHA synthase mutant generated by directed mutagenesis
Torrego-Solana, Noelia; Martin-Arjol, Ignacio; Bassas-Galia, Mònica; Diaz, Pilar; Manresa, Angeles
-based vector containing the lox flanked aacC1 ...
... gene [39]. pBlueScript II KS(+) replaced the  pUC18 
Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production
Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production
Wang, Xiaoqing; Ruan, Zhenhua; Boileau, Danielle; Sears, Barbara B.; Liu, Yan; Liao, Wei
part of the plasmid p699 by cutting both ...
... libraries with 46,462 clones were created using  pUC18 
Genomics of Xanthomonas citri and Related Species
Genomics of Xanthomonas citri and Related Species
Jalan, Neha; Yan, Qing; Kogenaru, Sunitha; Guo, Yinping; Jones, Jeffrey B.; Graham, James H.; Wang, Nian
to completely sequence the pathogen with 7X ...
... Jeffrey Leiden (University of Chicago) in the  pUC18 
β1 integrins show specific association with CD98 protein in low density membranes
β1 integrins show specific association with CD98 protein in low density membranes
Kolesnikova, Tatiana V; Mannion, Brian A; Berditchevski, Fedor; Hemler, Martin E
vector. ...
... suicide vector, AmpR [26] pUCGmlox AmpR, GmR,  pUC18 
The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis
The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis
Bazire, Alexis; Dufour, Alain
-based vector containing the lox flanked aacC1 ...
... total DNA extracted was < 21 blaTEM alleles (  pUC18 
Bacterial diversity in faeces from polar bear (Ursus maritimus) in Arctic Svalbard
Bacterial diversity in faeces from polar bear (Ursus maritimus) in Arctic Svalbard
Glad, Trine; Bernhardsen, Pål; Nielsen, Kaare M; Brusetti, Lorenzo; Andersen, Magnus; Aars, Jon; Sundset, Monica A
) per PCR sample [15]. ...
... product is ligated into a plasmid vector (e.g.,  pUC18 
Methods
Methods
Schiebel, Ralf; Hemleben, Christoph
), and transformed into competent Escherichia ...
... primer was complementary to regions of the  pUC18 
Virus-induced gene silencing in rice using a vector derived from a DNA virus
Virus-induced gene silencing in rice using a vector derived from a DNA virus
Purkayastha, Arunima; Mathur, Saloni; Verma, Vidhu; Sharma, Shweta; Dasgupta, Indranil
backbone of pRTBV203. ...