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... into the SmaI site of YEp352 This study p5921  pUC18 
Kre29p is a novel nuclear protein involved in DNA repair and mitotic fidelity in Candida glabrata
Kre29p is a novel nuclear protein involved in DNA repair and mitotic fidelity in Candida glabrata
Miyazaki, Taiga; Tsai, Huei-Fung; Bennett, John E.
containing a 4-kb BamHI/BglII hisG-URA3-hisG ...
... sequences of the selected cosmid clones. One  pUC18 
Isolation and characterization of alkane hydroxylases from a metagenomic library of Pacific deep-sea sediment
Isolation and characterization of alkane hydroxylases from a metagenomic library of Pacific deep-sea sediment
Xu, Meixiang; Xiao, Xiang; Wang, Fengping
library of random 2 kb fragments obtained by ...
... promoter studies pSVA5 (Albers et al. 2006), a  pUC18 
Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus
Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus
Lubelska, Joanna M.; Jonuscheit, Melanie; Schleper, Christa; Albers, Sonja-Verena; Driessen, Arnold J. M.
derivative, contained the pyrEF genes for ...
... I sites added on either end was reassembled in  pUC18 
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine
Tanzer, Fiona L; Shephard, Enid G; Palmer, Kenneth E; Burger, Marieta; Williamson, Anna-Lise; Rybicki, Edward P
by polymerase chain reaction (PCR) as detailed ...
... and was inserted into the HincII site of  pUC18 
NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme
NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme
Ohashi, Kazuto; Kawai, Shigeyuki; Koshimizu, Mari; Murata, Kousaku
, resulting in pMK1966. ...
... DNA of the pPL152 insert This study pTn13-20  pUC18 
Genetic analysis of the biosynthesis of non-ribosomal peptide- and polyketide-like antibiotics, iron uptake and biofilm formation by Bacillus subtilis A1/3
Genetic analysis of the biosynthesis of non-ribosomal peptide- and polyketide-like antibiotics, iron uptake and biofilm formation by Bacillus subtilis A1/3
Hofemeister, J.; Conrad, B.; Adler, B.; Hofemeister, B.; Feesche, J.; Kucheryava, N.; Steinborn, G.; Franke, P.; Grammel, N.; Zwintscher, A.; Leenders, F.; Hitzeroth, G.; Vater, J.
with excision DNA of transposon mutant BA13-20 ...
... to GFP under CaMV35S promoter in a modified  pUC18 
Identification of rice Di19 family reveals OsDi19-4 involved in drought resistance
Identification of rice Di19 family reveals OsDi19-4 involved in drought resistance
Wang, Lili; Yu, Changchun; Chen, Cong; He, Chunlan; Zhu, Yingguo; Huang, Wenchao
-based vector. ...
... experiments Jakoby et al. (2000)  DH5αmcr  pUC18 
Utilization of creatinine as an alternative nitrogen source in Corynebacterium glutamicum
Utilization of creatinine as an alternative nitrogen source in Corynebacterium glutamicum
Bendt, Anne K.; Beckers, Gabriele; Silberbach, Maike; Wittmann, Anja; Burkovski, Andreas
Control strain for gel retardation experiments ...
... μL of bacteria solution per construct. Include  pUC18 
shRNA-Induced Interferon-Stimulated Gene Analysis
shRNA-Induced Interferon-Stimulated Gene Analysis
Morral, Núria; Witting, Scott R.
as control for efficacy of the transformation, ...
... cloned into E. coli (DH10B strain) using  pUC18 
Molecular and Evolutionary Analysis of the Hd6 Photoperiod Sensitivity Gene Within Genus Oryza
Molecular and Evolutionary Analysis of the Hd6 Photoperiod Sensitivity Gene Within Genus Oryza
Yamane, Hiroko; Ito, Tomoko; Ishikubo, Harumi; Fujisawa, Masaki; Yamagata, Harumi; Kamiya, Kozue; Ito, Yukiyo; Hamada, Masao; Kanamori, Hiroyuki; Ikawa, Hiroshi; Katayose, Yuichi; Wu, Jianzhong; Sasaki, Takuji; Matsumoto, Takashi
vectors (TaKaRa). ...
... a shotgun library (SmaI fragments cloned in  PUC18 
Organization of the MASP2 locus and its expression profile in mouse and rat
Organization of the MASP2 locus and its expression profile in mouse and rat
Stover, Cordula M.; Lynch, Nicholas J.; Hanson, Steven J.; Windbichler, Michaela; Gregory, Simon G.; Schwaeble, Wilhelm J.
; Genterprise, Mainz, Germany) and partial ...
... it contains nucleotide sequences of the  pUC18 
Next-Generation Sequencing as a Tool for Detailed Molecular Characterisation of Genomic Insertions and Flanking Regions in Genetically Modified Plants: a Pilot Study Using a Rice Event Unauthorised in the EU
Next-Generation Sequencing as a Tool for Detailed Molecular Characterisation of Genomic Insertions and Flanking Regions in Genetically Modified Plants: a Pilot Study Using a Rice Event Unauthorised in the EU
Wahler, Daniela; Schauser, Leif; Bendiek, Joachim; Grohmann, Lutz
multiple cloning site, the aadA-gene, the ...
... pEFX90 by NcoI digestion and subcloned into the  pUC18 
Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function
Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function
Urcuqui-Inchima, Silvio; Castaño, Maria Eugenia; Hernandez-Verdun, Danièle; St-Laurent, Georges, III; Kumar, Ajit
vector upstream of and in-frame with the ...
... coli strain DH5α was used as the cloning host,  pUC18 
Variant cry1Ab entomocidal Bacillus thuringiensis toxin gene facilitates the recovery of an increased number of lepidopteran insect resistant independent rice transformants against yellow stem borer (Scirpophaga incertulus) inflicted damage
Variant cry1Ab entomocidal Bacillus thuringiensis toxin gene facilitates the recovery of an increased number of lepidopteran insect resistant independent rice transformants against yellow stem borer (Scirpophaga incertulus) inflicted damage
Dandapat, Abhijit; Bhattacharyya, Jagannath; Gayen, Srimonta; Chakraborty, Anirban; Banga, Anannya; Mukherjee, Rajeswari; Mandal, Chandi Charan; Hossain, Munshi Azad; Roy, Samarjit; Basu, Asitava; Sen, Soumitra Kumar
the cloning vector, pRSET A (Invitrogen) the T7 ...
... blunt ends and gel purified, then ligated into  pUC18 
Complete plastid genome sequences of Drimys, Liriodendron, and Piper: implications for the phylogenetic relationships of magnoliids
Complete plastid genome sequences of Drimys, Liriodendron, and Piper: implications for the phylogenetic relationships of magnoliids
Cai, Zhengqiu; Penaflor, Cynthia; Kuehl, Jennifer V; Leebens-Mack, James; Carlson, John E; dePamphilis, Claude W; Boore, Jeffrey L; Jansen, Robert K
plasmids. ...
... and the linearized molecules were ligated into  pUC18 
Role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in India
Role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in India
Sivalingam, P. N.; Varma, Anupam
. ...
... into Sma I digested and dephosphorylated  pUC18 
HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
Nadar, Muthukumar; Chan, Meng-Yu; Huang, Shi-Wei; Huang, Chin-Cheng; Tseng, Joseph T; Tsai, Ching-Hsiu
vector; and the positive clones were verified ...
... 1× NEBuffer 4. A carrier supercoiled plasmid (  pUC18 
Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications
Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications
Pham, Thang T.; Yin, Jun; Eid, John S.; Adams, Evan; Lam, Regina; Turner, Stephen W.; Loomis, Erick W.; Wang, Jun Yi; Hagerman, Paul J.; Hanes, Jeremiah W.
or pBR322), 500 ng, was added to each DNA ...
... (Supplementary Table 4). The construction of  pUC18 
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Waller, Jeffrey C.; Dhanoa, Preetinder K.; Schumann, Uwe; Mullen, Robert T.; Snedden, Wayne A.
/recA-CAT, consisting of the N-terminal ...
... with PstI, and cloned into the PstI site of  pUC18 
The Helicobacter pylori mutY homologue HP0142 is an antimutator gene that prevents specific C to A transversions
The Helicobacter pylori mutY homologue HP0142 is an antimutator gene that prevents specific C to A transversions
Kulick, Stefan; Moccia, Claudia; Kraft, Christian; Suerbaum, Sebastian
resulting in plasmid pSUS2500. ...
... were purified from Agarose gel, cloned in  pUC18 
A new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka
A new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka
Senanayake, D. M. J. B.; Jayasinghe, J. E. A. R. M.; Shilpi, S.; Wasala, S. K.; Mandal, Bikash
(Fermentas, Glen Burnie, Maryland, USA) or ...
... sheared genomic DNA using the plasmid vector  pUC18 
Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii
Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii
Zhao, Y.; Wang, H.; Hammond, R. W.; Jomantiene, R.; Liu, Q.; Lin, S.; Roe, B. A.; Davis, R. E.
. ...
... using the Nco I and Xba I sites into the  pUC18 
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
Schmidt, Thomas; Lenders, Malte; Hillebrand, Andrea; van Deenen, Nicole; Munt, Oliver; Reichelt, Rudolf; Eisenreich, Wolfgang; Fischer, Rainer; Prüfer, Dirk; Gronover, Christian Schulze
-based pAM vector containing the CaMV 35S ...
... and β-actin as internal positive control, and  pUC18 
Upregulation of Tissue and Urinary Nicotinamide N-Methyltransferase in Bladder Cancer: Potential for the Development of a Urine-Based Diagnostic Test
Upregulation of Tissue and Urinary Nicotinamide N-Methyltransferase in Bladder Cancer: Potential for the Development of a Urine-Based Diagnostic Test
Sartini, Davide; Muzzonigro, Giovanni; Milanese, Giulio; Pozzi, Valentina; Vici, Alexia; Morganti, Stefano; Rossi, Valentina; Mazzucchelli, Roberta; Montironi, Rodolfo; Emanuelli, Monica
as negative control. ...
... the SNR6 gene encoding U6 snRNA cloned into  pUC18 
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
Dery, Kenneth J.; Yean, Shyue-Lee; Lin, Ren-Jang
(7). 6. RNasin (Promega, Madison, WI). Use 10 U ...