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... and the linearized molecules were ligated into  pUC18 
Role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in India
Role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in India
Sivalingam, P. N.; Varma, Anupam
. ...
... into Sma I digested and dephosphorylated  pUC18 
HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
Nadar, Muthukumar; Chan, Meng-Yu; Huang, Shi-Wei; Huang, Chin-Cheng; Tseng, Joseph T; Tsai, Ching-Hsiu
vector; and the positive clones were verified ...
... 1× NEBuffer 4. A carrier supercoiled plasmid (  pUC18 
Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications
Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications
Pham, Thang T.; Yin, Jun; Eid, John S.; Adams, Evan; Lam, Regina; Turner, Stephen W.; Loomis, Erick W.; Wang, Jun Yi; Hagerman, Paul J.; Hanes, Jeremiah W.
or pBR322), 500 ng, was added to each DNA ...
... (Supplementary Table 4). The construction of  pUC18 
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Waller, Jeffrey C.; Dhanoa, Preetinder K.; Schumann, Uwe; Mullen, Robert T.; Snedden, Wayne A.
/recA-CAT, consisting of the N-terminal ...
... with PstI, and cloned into the PstI site of  pUC18 
The Helicobacter pylori mutY homologue HP0142 is an antimutator gene that prevents specific C to A transversions
The Helicobacter pylori mutY homologue HP0142 is an antimutator gene that prevents specific C to A transversions
Kulick, Stefan; Moccia, Claudia; Kraft, Christian; Suerbaum, Sebastian
resulting in plasmid pSUS2500. ...
... were purified from Agarose gel, cloned in  pUC18 
A new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka
A new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka
Senanayake, D. M. J. B.; Jayasinghe, J. E. A. R. M.; Shilpi, S.; Wasala, S. K.; Mandal, Bikash
(Fermentas, Glen Burnie, Maryland, USA) or ...
... sheared genomic DNA using the plasmid vector  pUC18 
Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii
Predicted ATP-binding cassette systems in the phytopathogenic mollicute Spiroplasma kunkelii
Zhao, Y.; Wang, H.; Hammond, R. W.; Jomantiene, R.; Liu, Q.; Lin, S.; Roe, B. A.; Davis, R. E.
. ...
... using the Nco I and Xba I sites into the  pUC18 
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
Schmidt, Thomas; Lenders, Malte; Hillebrand, Andrea; van Deenen, Nicole; Munt, Oliver; Reichelt, Rudolf; Eisenreich, Wolfgang; Fischer, Rainer; Prüfer, Dirk; Gronover, Christian Schulze
-based pAM vector containing the CaMV 35S ...
... and β-actin as internal positive control, and  pUC18 
Upregulation of Tissue and Urinary Nicotinamide N-Methyltransferase in Bladder Cancer: Potential for the Development of a Urine-Based Diagnostic Test
Upregulation of Tissue and Urinary Nicotinamide N-Methyltransferase in Bladder Cancer: Potential for the Development of a Urine-Based Diagnostic Test
Sartini, Davide; Muzzonigro, Giovanni; Milanese, Giulio; Pozzi, Valentina; Vici, Alexia; Morganti, Stefano; Rossi, Valentina; Mazzucchelli, Roberta; Montironi, Rodolfo; Emanuelli, Monica
as negative control. ...
... the SNR6 gene encoding U6 snRNA cloned into  pUC18 
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
Dery, Kenneth J.; Yean, Shyue-Lee; Lin, Ren-Jang
(7). 6. RNasin (Promega, Madison, WI). Use 10 U ...
... and 10mM 7dc-dGTP. 3. Some preparations of  pUC18 
Using the Automated DNA Sequencer
Using the Automated DNA Sequencer
Du, Zijin; Wilson, Richard K.
and pUC1 18 have deleted one nucleotide at the ...
... 3 kb short-insert Sanger library constructed in  pUC18 
Differences in sequencing technologies improve the retrieval of anammox bacterial genome from metagenomes
Differences in sequencing technologies improve the retrieval of anammox bacterial genome from metagenomes
Gori, Fabio; Tringe, Susannah G; Folino, Gianluigi; van Hijum, Sacha AFT; Op den Camp, Huub JM; Jetten, Mike SM; Marchiori, Elena
(henceforth referred as Shotgun), and 62 plates ...
... WAVE® DNA Sizing Standard for WAVE Systems  pUC18 
Screening for Genomic Rearrangements by Multiplex PCR/Liquid Chromatography
Screening for Genomic Rearrangements by Multiplex PCR/Liquid Chromatography
Houdayer, Claude; Dehainault, Catherine; Gauthier-Villars, Marion; Stoppa-Lyonnet, Dominique
HaeIII Digest (fragment sizes: 80, 102, 174, ...
... AatII-AatII fragment of Cmr was ligated with  pUC18 
Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1
Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1
Cho, Sukhyeong; Kim, Kyung Duk; Ahn, Jae-Hyung; Lee, Jinwon; Kim, Seon-Won; Um, Youngsoon
(GenScript, NJ, USA) for construction of ...
... the WT or mutant tRNAHis (GUG) was cloned into  pUC18 
Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase
Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase
Lee, Yi-Hsueh; Chang, Chia-Pei; Cheng, Yu-Ju; Kuo, Yi-Yi; Lin, Yeong-Shin; Wang, Chien-Chia
using complementary primers with a SmaI site on ...
... genetically modified. For example the standard  pUC18 
Site-Directed Mutagenesis and Gene Deletion Using Reverse Genetics
Site-Directed Mutagenesis and Gene Deletion Using Reverse Genetics
Muhl, Daniela; Filloux, Alain
or pUC19 plasmids are ColE1 replicon and do not ...
... from positive clones were subcloned into a  pUC18 
Sequence, evolution and ligand binding properties of mammalian Duffy antigen/receptor for chemokines
Sequence, evolution and ligand binding properties of mammalian Duffy antigen/receptor for chemokines
Tournamille, Christophe; Blancher, Antoine; Le Van Kim, Caroline; Gane, Pierre; Apoil, Pol André; Nakamoto, Wilson; Cartron, Jean Pierre; Colin, Yves
EcoRI vector and sequenced using the ALFexpress ...
... genome insert was released from the vector  pUC18 
Infectivity and the phylogenetic relationship of a mastrevirus causing chickpea stunt disease in India
Infectivity and the phylogenetic relationship of a mastrevirus causing chickpea stunt disease in India
Kanakala, S.; Sakhare, A.; Verma, H. N.; Malathi, V. G.
by digestion with HindIII and BglI. 50 ng of ...
... pBI121 and pCAMBIA1301 and cloning vector  pUC18 
Genetic Engineering for Improving the Performance of Biotic Biological Control Agents
Genetic Engineering for Improving the Performance of Biotic Biological Control Agents
Narayanasamy, P.
were employed to successfully construct ...
... in size from 2.5 to 5 Kb were ligated to  PUC18 
Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300
Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300
Franchel, Jérôme; Bouzidi, Mohamed Fouad; Bronner, Gisèle; Vear, Felicity; Nicolas, Paul; Mouzeyar, Said
and transformed into E. coli using Electro Ten ...
... pM151 (Manivasakam and Schiestl 1998), a  pUC18 
Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae
Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae
Friedl, Anna A.; Kiechle, Markus; Maxeiner, Horst G.; Schiestl, Robert H.; Eckardt-Schupp, Friederike
derivative containing the URA3 gene, served as ...
... from D. aromatica strain RCB and inserted into  pUC18 
Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation
Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation
Salinero, Kennan Kellaris; Keller, Keith; Feil, William S; Feil, Helene; Trong, Stephan; Di Bartolo, Genevieve; Lapidus, Alla
, pCUGIblu21, and pcc1Fos vectors, ...
... 3D8 scFv protein was incubated with dsDNA (  pUC18 
Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation
Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation
Jung, Yuchul; Rhee, Yong; Auh, Chung-Kyoon; Shim, HyeKyung; Choi, Jung-Jin; Kwon, Suk-Tae; Yang, Joo-Sung; Kim, Donggiun; Kwon, Myung-Hee; Kim, Yong-Sung; Lee, Sukchan
plasmid DNA) upto 120 min at 37°C and then the ...
... Kit (Biobasic Inc.) and ligated into the  pUC18 
Development, characterization and utilization of GenBank microsatellite markers in Phyllostachys pubescens and related species
Development, characterization and utilization of GenBank microsatellite markers in Phyllostachys pubescens and related species
Tang, Ding-Qin; Lu, Jiang-Jie; Fang, Wei; Zhang, Shan; Zhou, Ming-Bing
vector (TaKaRa, Japan). ...
... pOCT Octane (component of crude oil) P. putida  pUC18 
Biodegradation and Bioremediation of Organic Chemical Pollutants by Pseudomonas
Biodegradation and Bioremediation of Organic Chemical Pollutants by Pseudomonas
Kahlon, Rachhpal S.
Dibenzothiophene naphthalene, phenanthrene P. ...