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... 1973) was cloned into the plasmid vector  pUC18 
Diversity of DMSP transport in marine bacteria, revealed by genetic analyses
Diversity of DMSP transport in marine bacteria, revealed by genetic analyses
Sun, Lei; Curson, Andrew R. J.; Todd, Jonathan D.; Johnston, Andrew W. B.
(Messing et al. 1983) to form pBIO1609. This ...
... vector with multiple-cloning site from  pUC18 
Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3
Wang, Yan; Nie, Maiqian; Wan, Yi; Tian, Xiaoting; Nie, Hongyun; Zi, Jing; Ma, Xia
, AmpR Hoang et al. 1998  pHpΏ45 Smr/Spcr gene ...
... 2012). Many plasmids, including T-vector (e.g.  pUC18 
Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression
Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression
Xu, Jian-zhong; Zhang, Wei-guo
and pMD™18-T), mobilizable plasmid (e.g. ...
... with a 1-bp ladder formed by a sequence of  pUC18 
Nuclear and chloroplast DNA reassessment of the origin of Indian potato varieties and its implications for the origin of the early European potato
Nuclear and chloroplast DNA reassessment of the origin of Indian potato varieties and its implications for the origin of the early European potato
Spooner, D. M.; Nuñez, J.; Rodríguez, F.; Naik, P. S.; Ghislain, M.
-forward primer. ...
... and hence were ligated with EcoRI-digested  pUC18 
Puf operon sequences and inferred structures of light-harvesting complexes of three closely related Chromatiaceae exhibiting different absorption characteristics
Puf operon sequences and inferred structures of light-harvesting complexes of three closely related Chromatiaceae exhibiting different absorption characteristics
Rücker, Ovidiu; Köhler, Anne; Behammer, Beate; Sichau, Katja; Overmann, Jörg
. ...
... between groups (P > 0.05). Negative controls (  PUC18 
Effects of Estrogen on Messenger Ribonucleic Acid Expression Patterns of Extracellular Matrix Proteases in the Primate Colon
Effects of Estrogen on Messenger Ribonucleic Acid Expression Patterns of Extracellular Matrix Proteases in the Primate Colon
Mills, Steven; Young, Kelly A.; Whiteford, Mark H.
viral RNA and blank spots) showed very low to ...
... by Sau3AI and subcloned into the BamHI site of  pUC18 
A physical map of large segments of pig Chromosome 7q11–q14: comparative analysis with human Chromosome 6p21
A physical map of large segments of pig Chromosome 7q11–q14: comparative analysis with human Chromosome 6p21
Barbosa, Angela; Demeure, Olivier; Urien, Céline; Milan, Denis; Chardon, Patrick; Renard, Christine
. ...
... et al. 1999b). pTaS3 was derived from the  pUC18 
Wheat gene TaS3 contributes to powdery mildew susceptibility
Wheat gene TaS3 contributes to powdery mildew susceptibility
Li, Shaohui; Ji, Rui; Dudler, Robert; Yong, Mingli; Deng, Qide; Wang, Zhengyi; Hu, Dongwei
-based plant expression vector pGY1 (Shinshi et ...
... and hydroxyl radical-induced nicking in plasmid  pUC18 
Allium cepa
Allium cepa
Lim, T. K.
DNA (Singh et al. 2009). Kim et al. (2013) ...
... of DNA bonds to radiation damage by irradiating  pUC18 
Tip-enhanced Raman spectroscopy: principles and applications
Tip-enhanced Raman spectroscopy: principles and applications
Kumar, Naresh; Mignuzzi, Sandro; Su, Weitao; Roy, Debdulal
circular plasmid DNA as a thin-film in an ...
... −89 bp) and core promoter (−89 to +1 bp) in a  pUC18 
Grapevines engineered to express cisgenic Vitis vinifera thaumatin-like protein exhibit fungal disease resistance
Grapevines engineered to express cisgenic Vitis vinifera thaumatin-like protein exhibit fungal disease resistance
Dhekney, Sadanand A.; Li, Zhijian T.; Gray, Dennis J.
-based intermediate plasmid to create pDVT. ...
... shows location of TSP-1-specific mRNA (k/7).  PUC18 
The proangiogenic phenotype of human tumor-derived endothelial cells depends on thrombospondin-1 downregulation via phosphatidylinositol 3-kinase/Akt pathway
The proangiogenic phenotype of human tumor-derived endothelial cells depends on thrombospondin-1 downregulation via phosphatidylinositol 3-kinase/Akt pathway
Bussolati, Benedetta; Assenzio, Barbara; Deregibus, Maria Chiara; Camussi, Giovanni
plasmid DNA and blank spots served as negative ...
... Pasteur, 25 rue du Dr Roux, Paris, France  pUC18 
Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
Armshaw, Patricia; Pembroke, J Tony
AmR template for deletion mutant construction ...
... of human genomic DNA and cloned into  pUC18 
Identification and analysis of the promoter region of the human DHCR24 gene: involvement of DNA methylation and histone acetylation
Identification and analysis of the promoter region of the human DHCR24 gene: involvement of DNA methylation and histone acetylation
Drzewinska, Joanna; Walczak-Drzewiecka, Aurelia; Ratajewski, Marcin
using the HincII blunt end restriction ...
... E. coli This study pSU2718 Hae II fragment of  pUC18 
Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid
Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid
Sun, Zhoutong; Ning, Yuanyuan; Liu, Lixia; Liu, Yingmiao; Sun, Bingbing; Jiang, Weihong; Yang, Chen; Yang, Sheng
containing lacZα and MCS, p15A replicon with ...
... encodes the SAI/II antigen, were removed from  pUC18 
Generation of human antibody fragments against Streptococcus mutansusing a phage display chain shuffling approach
Generation of human antibody fragments against Streptococcus mutansusing a phage display chain shuffling approach
Kupper, Michael B; Huhn, Michael; Spiegel, Holger; Ma, Julian KC; Barth, Stefan; Fischer, Rainer; Finnern, Ricarda
as a SfiI/NotI fragment and inserted into the ...
... into the SmaI site of YEp352 This study p5921  pUC18 
Kre29p is a novel nuclear protein involved in DNA repair and mitotic fidelity in Candida glabrata
Kre29p is a novel nuclear protein involved in DNA repair and mitotic fidelity in Candida glabrata
Miyazaki, Taiga; Tsai, Huei-Fung; Bennett, John E.
containing a 4-kb BamHI/BglII hisG-URA3-hisG ...
... sequences of the selected cosmid clones. One  pUC18 
Isolation and characterization of alkane hydroxylases from a metagenomic library of Pacific deep-sea sediment
Isolation and characterization of alkane hydroxylases from a metagenomic library of Pacific deep-sea sediment
Xu, Meixiang; Xiao, Xiang; Wang, Fengping
library of random 2 kb fragments obtained by ...
... promoter studies pSVA5 (Albers et al. 2006), a  pUC18 
Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus
Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus
Lubelska, Joanna M.; Jonuscheit, Melanie; Schleper, Christa; Albers, Sonja-Verena; Driessen, Arnold J. M.
derivative, contained the pyrEF genes for ...
... I sites added on either end was reassembled in  pUC18 
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine
Tanzer, Fiona L; Shephard, Enid G; Palmer, Kenneth E; Burger, Marieta; Williamson, Anna-Lise; Rybicki, Edward P
by polymerase chain reaction (PCR) as detailed ...
... and was inserted into the HincII site of  pUC18 
NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme
NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme
Ohashi, Kazuto; Kawai, Shigeyuki; Koshimizu, Mari; Murata, Kousaku
, resulting in pMK1966. ...
... DNA of the pPL152 insert This study pTn13-20  pUC18 
Genetic analysis of the biosynthesis of non-ribosomal peptide- and polyketide-like antibiotics, iron uptake and biofilm formation by Bacillus subtilis A1/3
Genetic analysis of the biosynthesis of non-ribosomal peptide- and polyketide-like antibiotics, iron uptake and biofilm formation by Bacillus subtilis A1/3
Hofemeister, J.; Conrad, B.; Adler, B.; Hofemeister, B.; Feesche, J.; Kucheryava, N.; Steinborn, G.; Franke, P.; Grammel, N.; Zwintscher, A.; Leenders, F.; Hitzeroth, G.; Vater, J.
with excision DNA of transposon mutant BA13-20 ...
... to GFP under CaMV35S promoter in a modified  pUC18 
Identification of rice Di19 family reveals OsDi19-4 involved in drought resistance
Identification of rice Di19 family reveals OsDi19-4 involved in drought resistance
Wang, Lili; Yu, Changchun; Chen, Cong; He, Chunlan; Zhu, Yingguo; Huang, Wenchao
-based vector. ...
... experiments Jakoby et al. (2000)  DH5αmcr  pUC18 
Utilization of creatinine as an alternative nitrogen source in Corynebacterium glutamicum
Utilization of creatinine as an alternative nitrogen source in Corynebacterium glutamicum
Bendt, Anne K.; Beckers, Gabriele; Silberbach, Maike; Wittmann, Anja; Burkovski, Andreas
Control strain for gel retardation experiments ...
... μL of bacteria solution per construct. Include  pUC18 
shRNA-Induced Interferon-Stimulated Gene Analysis
shRNA-Induced Interferon-Stimulated Gene Analysis
Morral, Núria; Witting, Scott R.
as control for efficacy of the transformation, ...