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... an Escherichia coli plasmid, such as  pUC18 
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis
Bifani, Pablo; Kurepina, Natalia; Mathema, Barun; Wang, Xiao-Ming; Kreiswirth, Barry
, containing the cloned 3′-IS6110 fragment as ...
... were ligated either into the BamHI site of  pUC18 
Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias
Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias
van der Walt, Eric; Martin, Darren P; Varsani, Arvind; Polston, Jane E; Rybicki, Edward P
using standard techniques (all clones labelled ...
... of a 311 bp fragment, which was cloned into the  pUC18 
Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881
Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881
Ryzhkov, Pavel M.; Ostermann, Kai; Rödel, Gerhard
vector and sequenced. ...
... B − ),gal(DE3) Novagen Plasmids  pUC18 
Identification of α-d-glucose-1-phosphate cytidylyltransferase involved in Ebosin biosynthesis of Streptomyces sp. 139
Identification of α-d-glucose-1-phosphate cytidylyltransferase involved in Ebosin biosynthesis of Streptomyces sp. 139
Qi, Xiao-Qiang; Sun, Qing-Li; Bai, Li-Ping; Shan, Jun-Jie; Zhang, Yang; Zhang, Ren; Li, Yuan
E. coli general cloning vector with multiple ...
... mechanically sheared DNA (2.5–5 Kb) into  pUC18 
Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species
Legault, Boris A; Lopez-Lopez, Arantxa; Alba-Casado, Jose Carlos; Doolittle, W Ford; Bolhuis, Henk; Rodriguez-Valera, Francisco; Papke, R Thane
. ...
... was inserted into the PstI site of plasmid  pUC18 
Heterologous Production of Thermostable Proteins and Enzymes
Heterologous Production of Thermostable Proteins and Enzymes
Sakuraba, Haruhiko; Ohshima, Toshihisa
, which was then used to transform E. coli ...
... DNA fragment of 4,125 bp was ligated into  pUC18 
Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus
Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus
Sugareva, Venelina; Härtl, Albert; Brock, Matthias; Hübner, Katrin; Rohde, Manfred; Heinekamp, Thorsten; Brakhage, Axel A.
, also digested with EcoRI, yielding plasmid ...
... SSR fragment size ladders We initially used a  pUC18 
Robust and highly informative microsatellite-based genetic identity kit for potato
Robust and highly informative microsatellite-based genetic identity kit for potato
Ghislain, Marc; Núñez, Jorge; Rosario Herrera, María; Pignataro, José; Guzman, Frank; Bonierbale, Merideth; Spooner, David M.
sequencing reaction or a IRDye-50–350 size ...
... + (Kilaru et al. 2006) and p004iGM3 which is a  pUC18 
Paradoxical performance of tryptophan synthase gene trp1+ in transformations of the basidiomycete Coprinopsis cinerea
Paradoxical performance of tryptophan synthase gene trp1+ in transformations of the basidiomycete Coprinopsis cinerea
Dörnte, Bastian; Kües, Ursula
construct containing an enhanced gfp (egfp) ...
... amplified insert This study  pUCGmlox ApR, GmR,  pUC18 
Hydroxy-fatty acid production in a Pseudomonas aeruginosa 42A2 PHA synthase mutant generated by directed mutagenesis
Hydroxy-fatty acid production in a Pseudomonas aeruginosa 42A2 PHA synthase mutant generated by directed mutagenesis
Torrego-Solana, Noelia; Martin-Arjol, Ignacio; Bassas-Galia, Mònica; Diaz, Pilar; Manresa, Angeles
-based vector containing the lox flanked aacC1 ...
... gene [39]. pBlueScript II KS(+) replaced the  pUC18 
Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production
Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production
Wang, Xiaoqing; Ruan, Zhenhua; Boileau, Danielle; Sears, Barbara B.; Liu, Yan; Liao, Wei
part of the plasmid p699 by cutting both ...
... libraries with 46,462 clones were created using  pUC18 
Genomics of Xanthomonas citri and Related Species
Genomics of Xanthomonas citri and Related Species
Jalan, Neha; Yan, Qing; Kogenaru, Sunitha; Guo, Yinping; Jones, Jeffrey B.; Graham, James H.; Wang, Nian
to completely sequence the pathogen with 7X ...
... Jeffrey Leiden (University of Chicago) in the  pUC18 
β1 integrins show specific association with CD98 protein in low density membranes
β1 integrins show specific association with CD98 protein in low density membranes
Kolesnikova, Tatiana V; Mannion, Brian A; Berditchevski, Fedor; Hemler, Martin E
vector. ...
... suicide vector, AmpR [26] pUCGmlox AmpR, GmR,  pUC18 
The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis
The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis
Bazire, Alexis; Dufour, Alain
-based vector containing the lox flanked aacC1 ...
... total DNA extracted was < 21 blaTEM alleles (  pUC18 
Bacterial diversity in faeces from polar bear (Ursus maritimus) in Arctic Svalbard
Bacterial diversity in faeces from polar bear (Ursus maritimus) in Arctic Svalbard
Glad, Trine; Bernhardsen, Pål; Nielsen, Kaare M; Brusetti, Lorenzo; Andersen, Magnus; Aars, Jon; Sundset, Monica A
) per PCR sample [15]. ...
... product is ligated into a plasmid vector (e.g.,  pUC18 
Methods
Methods
Schiebel, Ralf; Hemleben, Christoph
), and transformed into competent Escherichia ...
... primer was complementary to regions of the  pUC18 
Virus-induced gene silencing in rice using a vector derived from a DNA virus
Virus-induced gene silencing in rice using a vector derived from a DNA virus
Purkayastha, Arunima; Mathur, Saloni; Verma, Vidhu; Sharma, Shweta; Dasgupta, Indranil
backbone of pRTBV203. ...
... total) and one positive control using 0.5 μL of  pUC18 
Adenovirus Expressing p53
Adenovirus Expressing p53
Holmes, Matthew; Rosenberg, Elizabeth; Valerie, Kristoffer
control from Stratagene (provided with ...
... (see below). PCR products were cloned into  pUC18 
Proteomic analysis reveals the wide-ranging effects of the novel, iron-responsive regulator RirA in Rhizobium leguminosarum bv. viciae
Proteomic analysis reveals the wide-ranging effects of the novel, iron-responsive regulator RirA in Rhizobium leguminosarum bv. viciae
Todd, Jonathan D.; Sawers, Gary; Johnston, Andrew W. B.
, then into the wide-host-range promoter-probe ...
... vector; Tc Minder et al. (2000)    pUC18 
Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum
Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum
Hacker, Stephanie; Gödeke, Julia; Lindemann, Andrea; Mesa, Socorro; Pessi, Gabriella; Narberhaus, Franz
High-copy cloning vector; Ap Vieira and Messing ...
... fragments were ligated into the SmaI site of a  pUC18 
The complete mitochondrial genome of the Antarctic springtail Cryptopygus antarcticus (Hexapoda: Collembola)
The complete mitochondrial genome of the Antarctic springtail Cryptopygus antarcticus (Hexapoda: Collembola)
Carapelli, Antonio; Comandi, Sara; Convey, Peter; Nardi, Francesco; Frati, Francesco
cloning vector using the Fast-Link DNA ligation ...
... HindIII and PstI (MBI Ltd), was cloned into  pUC18 
The transcriptional activity of WT1 gene promoter and enhancer in cell lines with diverse tissue origin
The transcriptional activity of WT1 gene promoter and enhancer in cell lines with diverse tissue origin
Hu, Shaoyan; Chen, Zixing; Gu, Weiying; Chen, Ruihua; Zhao, Ye; Cen, Jiannong
and sequenced (GenBank accession no. ...
... were grown and sequenced with the M13-  pUC18 
In vitro infectivity and differential gene expression of Leishmania infantum metacyclic promastigotes: negative selection with peanut agglutinin in culture versus isolation from the stomodeal valve of Phlebotomus perniciosus
In vitro infectivity and differential gene expression of Leishmania infantum metacyclic promastigotes: negative selection with peanut agglutinin in culture versus isolation from the stomodeal valve of Phlebotomus perniciosus
Alcolea, Pedro J.; Alonso, Ana; Degayón, María A.; Moreno-Paz, Mercedes; Jiménez, Maribel; Molina, Ricardo; Larraga, Vicente
primers and assembled as described [26]. ...
... ahyRI′:: luxCDABE (Swift et al. 1997)  pSB1075  pUC18 
Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production
Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production
Hothersall, Joanne; Murphy, Annabel C.; Iqbal, Zafar; Campbell, Genevieve; Stephens, Elton R.; Wu, Ji’en; Cooper, Helen; Atkinson, Steve; Williams, Paul; Crosby, John; Willis, Christine L.; Cox, Russell J.; Simpson, Thomas J.; Thomas, Christopher M.
containing luxRI′::luxCDABE (Winson et al. ...
... a vector was constructed based on  pUC18 
Biotechnological production of astaxanthin with Phaffia rhodozyma/Xanthophyllomyces dendrorhous
Biotechnological production of astaxanthin with Phaffia rhodozyma/Xanthophyllomyces dendrorhous
Schmidt, Isabell; Schewe, Hendrik; Gassel, Sören; Jin, Chao; Buckingham, John; Hümbelin, Markus; Sandmann, Gerhard; Schrader, Jens
which allows plasmid propagation in E. coli ...