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... with the appropriate antibiotic. The pYES2,  pUC18 
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Expression and Characteristics of an Endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
Huang, Xiao-mei; Li, Qing-qing; Chen, Xiu-ling; Fan, Jin-xia; Xu, Xiu-hong; Sun, Xu-dong; Li, Dong-yu; Zhao, Hong-xiao
, pPIC9K, and pCSN43 plasmids were used for the ...
... 621H pGOX3, and E. coli cloning vector  pUC18 
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans
Zhang, Lin; Lin, Jinping; Ma, Yushu; Wei, Dongzhi; Sun, Ming
. ...
... cosmid was further constructed into a  pUC18 
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain
Wang, Jianjun; Zhu, Yaxin; Zhao, Guogang; Zhu, Junge; Wu, Sheng
plasmid library and screened by the same ...
... the heme proteins converted the supercoiled  pUC18 
Endonuclease-like activity of heme proteins
Endonuclease-like activity of heme proteins
Tan, Willy B.; Cheng, Wunhuey; Webber, Andrew; Bhambhani, Akhilesh; Duff, Michael R.; Kumar, Challa V.; McLendon, George L.
DNA into nicked circular and linear DNA. ...
... below). Controls Nick-translated biotin-labeled  pUC18 
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens
Evans, Mark F; Aliesky, Holly A; Cooper, Kumarasen
plasmid was used as a control for non-specific ...
... Simon et al., 1983 pT7Blue(R) Apr, lacZ Novagen  pUC18 
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria
Shintani, Masaki; Yoshida, Takako; Habe, Hiroshi; Omori, Toshio; Nojiri, Hideaki
/19 Apr, lacZ, pMB9 replicon Sambrook and ...
... In contrast, the high copy number plasmid  pUC18 
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Improved delivery of the OVA-CD4 peptide to T helper cells by polymeric surface display on Salmonella
Zhang, Junjie; De Masi, Leon; John, Beena; Chen, Wenxin; Schifferli, Dieter M
-OVA that constitutively expressed the ...
... pIJ101 aac(3)IV ermEp; oriT RK2 [16] pL98 7.4  pUC18 
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production
Rebets, Yuriy; Kormanec, Jan; Luzhetskyy, Andriy; Bernaerts, Kristel; Anné, Jozef
aac(3)IV pIJ101 aac(3)IV ssrAp; oriT RK2 [16] ...
... RNAP was established. The template plasmid is a  pUC18 
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium
Stammen, Simon; Schuller, Franziska; Dietrich, Sylvia; Gamer, Martin; Biedendieck, Rebekka; Jahn, Dieter
derivative, which enables blue/white screening ...
... of 50 μL at 37 °C for 2.5 h. Subsequently,  pUC18 
SeGSTo, a novel glutathione S-transferase from the beet armyworm (Spodoptera exigua), involved in detoxification and oxidative stress
SeGSTo, a novel glutathione S-transferase from the beet armyworm (Spodoptera exigua), involved in detoxification and oxidative stress
Xu, Pengfei; Han, Ningning; Kang, Tinghao; Zhan, Sha; Lee, Kwang Sik; Jin, Byung Rae; Li, Jianhong; Wan, Hu
super-coiled plasmid DNA (1000 ng) was added to ...
... enzymes were purchased from TAKARA. Plasmids of  pUC18 
Cloning and Characterization of a Sucrose Isomerase from Erwinia rhapontici NX-5 for Isomaltulose Hyperproduction
Cloning and Characterization of a Sucrose Isomerase from Erwinia rhapontici NX-5 for Isomaltulose Hyperproduction
Li, Sha; Cai, Heng; Qing, Yujia; Ren, Ben; Xu, Hong; Zhu, Hongyang; Yao, Jun
and pET22b were obtained from Novagen Co., ...
... Plasmid Characteristics Reference and source  pUC18 
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans
Kasuga, Kano; Nitta, Atsushi; Kobayashi, Masayuki; Habe, Hiroshi; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio; Kojima, Ikuo
/19 bla, lacZ′, pMB9 replicon Yanisch-Perron et ...
... CA, USA). Nuclease activity assay Plasmid  pUC18 
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity
Wu, Shih-Lu; Li, Chia-Cheng; Chen, Jaw-Chyun; Chen, Yi-Jin; Lin, Ching-Ting; Ho, Tin-Yun; Hsiang, Chien-Yun
dsDNA, preparing with the Qiagen plasmid midi ...
... for construction of GFP derivatives. Plasmid  pUC18 
Surface Display of GFP by Pseudomonas Syringae Truncated Ice Nucleation Protein in Attenuated Vibrio Anguillarum Strain
Surface Display of GFP by Pseudomonas Syringae Truncated Ice Nucleation Protein in Attenuated Vibrio Anguillarum Strain
Xu, Yuzhou; Liu, Qin; Zhou, Lingyun; Yang, Zhao; Zhang, Yuanxing
(TaKaRa, Japan) was used as a parent plasmid ...
... any plasmid encoding the LacZα-peptide such as  pUC18 
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis
Refaii, Abdalla Al; Jean-Hervé, Alix
/19 or pWSK129 (13) can be used. ...
... relA1 Clontech, Heidelberg, Germany Plasmids  pUC18 
The response of Serratia marcescens JG to environmental changes by quorum sensing system
The response of Serratia marcescens JG to environmental changes by quorum sensing system
Sun, Shu-Jing; Liu, Hui-Jun; Weng, Cai-Hong; Lai, Chun-Fen; Ai, Liu-Ying; Liu, Yu-Chen; Zhu, Hu
pBR322-derived cloning vector, Lac+, Apr MBI ...
... cloning of PCR products (Promega, Madison, WI);  pUC18 
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions
Zoulim, Fabien; Tong, ShuPing; Trépo, Christian
or pUC19 for whole HBV-genome cloning (New ...
... concentrations: 3, 6, 9, 12 and 15 μM), 1 μL of  pUC18 
Synthesis, crystal structure and characterization of new biologically active Cu(II) complexes with ligand derived from N-substituted sulfonamide
Synthesis, crystal structure and characterization of new biologically active Cu(II) complexes with ligand derived from N-substituted sulfonamide
HANGAN, ADRIANA CORINA; TURZA, ALEXANDRU; STAN, ROXANA LIANA; SEVASTRE, BOGDAN; PÁLL, EMÖKE; CETEAN, SÎNZIANA; OPREAN, LUMINIŢA SIMONA
DNA solution (0.25 μg/ μL, 750 μM in base ...
... containing a large spacer DNA sequence of  pUC18 
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
Wang, Baoli; Li, Xiaoxia; Zheng, Fang; Liu, Rui; Quan, Jinxing; Liang, Hui; Guo, Shanyi; Guo, Gang; Zhang, Jingyu
. ...
... along with chrysotile asbestos fibers and  pUC18 
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Quantitative detection of asbestos fiber in gravelly sand using elastic body-exposure method
Yoshida, Naoto; Takebe, Kazuki
donor DNA, was spread over the surface of an LB ...
... in an adverse manner. In this experiment,  pUC18 
Cloning and Transformation
Cloning and Transformation
Das, Surajit; Dash, Hirak Ranjan
and lambda DNA (λDNA) will be double digested ...
... and Rp107; Additional file 4) and the plasmid  pUC18 
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex
Hwang, Yeen Ting; McCartney, Andrew W; Gidda, Satinder K; Mullen, Robert T
/CIRV (purchased from the American Type Culture ...
... Plasmids pCC1FOS Cloning vector; Chlr Epicentre  pUC18 
Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
JunGang, Li; KeGui, Zhang; WenJun, Han
Cloning vector; Apr Takara pET28a Expression ...
... Genomic insertion of eGFP This study Plasmids    pUC18 
A three-step method for analysing bacterial biofilm formation under continuous medium flow
A three-step method for analysing bacterial biofilm formation under continuous medium flow
Schmutzler, Karolin; Schmid, Andreas; Buehler, Katja
_mini-Tn7-Gm-T7 ...
... digested with HindIII/PstI and ligated into the  pUC18 
Bm65 is Essential for the Propagation of Bombyx mori Nucleopolyhedrovirus
Bm65 is Essential for the Propagation of Bombyx mori Nucleopolyhedrovirus
Tang, Qi; Li, Guohui; Yao, Qin; Chen, Liang; Feng, Fan; Yuan, Yi; Chen, Keping
plasmid to generate the recombinant plasmid ...