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... (1991)  CaMV35S-sGFP (S65T)-NOS3′ GFP in  pUC18 
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
Kiyono, Masako; Oka, Yumiko; Sone, Yuka; Tanaka, Michitaka; Nakamura, Ryosuke; Sato, Masa H.; Pan-Hou, Hidemitsu; Sakabe, Kou; Inoue, Ken-ichiro
Uemura et al. (2004)  SYP111/KOLLE GFP-SYP111 ...
... (carrying wildtype dnaQ), and the empty vector  pUC18 
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production
Luan, Guodong; Cai, Zhen; Li, Yin; Ma, Yanhe
, was transformed into E. coli cells, ...
... of the trc promoter This work Plasmids    pUC18 
Succinic Acid Production from Corn Cob Hydrolysates by Genetically Engineered Corynebacterium glutamicum
Succinic Acid Production from Corn Cob Hydrolysates by Genetically Engineered Corynebacterium glutamicum
Wang, Chen; Zhang, Hengli; Cai, Heng; Zhou, Zhihui; Chen, Yilu; Chen, Yali; Ouyang, Pingkai
Apr; a-lac/MCS M13 ori Takara   ...
... respectively, and subcloned into the plasmid  pUC18 
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster
Yang, Xue-Peng; Zhong, Gui-Fang; Lin, Jin-Ping; Mao, Duo-Bin; Wei, Dong-Zhi
between the EcoRI–PstI sites downstream of the ...
... fragments of 1,769 and 676 bp from  pUC18 
Genome-specific primer sets for starch biosynthesis genes in wheat
Genome-specific primer sets for starch biosynthesis genes in wheat
Blake, N. K.; Sherman, J. D.; Dvořák, J.; Talbert, L. E.
digested with RsaI. ...
... imm downstream of the tet R promoter.  pUC18 
Stationary phase-like properties of the bacteriophage λ Rex exclusion phenotype
Stationary phase-like properties of the bacteriophage λ Rex exclusion phenotype
Slavcev, R. A.; Hayes, S.
and pUC19 were obtained from New England ...
... of the elongation factor 1α (EF 1α) promoter.  pUC18 
shRNA-Mediated Gene Knockdown in Skeletal Muscle
shRNA-Mediated Gene Knockdown in Skeletal Muscle
Golzio, Muriel; Escoffre, Jean-Michel; Teissié, Justin
plasmid contains the cDNA of subunit α of LacZ ...
... that all complexes used converted supercoiled  pUC18 
Studies on synthesis, structure, and DNA cleaving of homo-dinuclear Mn(II), Cu(II), Ni(II), and Zn(II) complexes with a heterocycle-based macroacyclic Schiff base
Studies on synthesis, structure, and DNA cleaving of homo-dinuclear Mn(II), Cu(II), Ni(II), and Zn(II) complexes with a heterocycle-based macroacyclic Schiff base
Baran, Talat; Karaoğlu, Kaan; Serbest, Kerim; Ağır, Hülya; Sandallı, Cemal; Değirmencioğlu, İsmail
plasmid DNA to its nicked or linear form. ...
... Korea and used as a host for gene expression.  pUC18 
Enhanced activity of meso-secondary alcohol dehydrogenase from Klebsiella species by codon optimization
Enhanced activity of meso-secondary alcohol dehydrogenase from Klebsiella species by codon optimization
Lee, Soojin; Kim, Borim; Oh, Minkyu; Kim, Youngrok; Lee, Jinwon
(Takara Shuzo, Kyoto, Japan) was used as the ...
... GFP, the ORF was amplified by PCR and cloned in  pUC18 
AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family
AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family
Weichert, Annett; Brinkmann, Christopher; Komarova, Nataliya Y.; Dietrich, Daniela; Thor, Kathrin; Meier, Stefan; Suter Grotemeyer, Marianne; Rentsch, Doris
-spGFP6 and ...
... from the pDONR 221 plasmid, cloned into the  pUC18 
pELMO, an optimised in-house cloning vector
pELMO, an optimised in-house cloning vector
Ramos, Andrea E.; Muñoz, Marina; Moreno-Pérez, Darwin A.; Patarroyo, Manuel A.
vector’s multiple cloning site (Thermo ...
...  pPM Cmr, Emr; pHP13 (P43b ::mdh) This study    pUC18 
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion
Mu, Li; Wen, Jianping
E. coli cloning vector; Ampr Laboratory stock ...
... in this study Store in our lab [21] Plasmids    pUC18 
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Liao, Yan; Li, Chun-mei; Chen, Hui; Wu, Qi; Shan, Zhi; Han, Xue-yi
-phyA m Modified phyA fragment cloned into the ...
... with nitrogen for 15 min prior to measurement.  pUC18 
Evaluation of antiproliferative activities and apoptosis induction caused by copper(II)–benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cells
Evaluation of antiproliferative activities and apoptosis induction caused by copper(II)–benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cells
González-Álvarez, Marta; Alzuet, Gloria; Borrás, Joaquín; Castillo-Agudo, Lucas; Montejo-Bernardo, Jose Manuel; Gutiérrez-Rodríguez, Angel; García-Granda, Santiago
DNA cleavage Reactions were performed by mixing ...
... Preparation of Plasmid Vector 1. Plasmid  pUC18 
Monitoring Drug Resistance for Herpesviruses
Monitoring Drug Resistance for Herpesviruses
Andrei, Graciela; Fiten, Pierre; Clercq, Erik; Snoeck, Robert; Opdenakker, Ghislain
(Pharmacia Biotech). 2. BamHI restriction ...
... along with chrysotile asbestos fibers and  pUC18 
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA
Yoshida, N.; Saeki, Y.
donor DNA, was spread over the surface of a ...
... fragment was subcloned into the HindIII site of  pUC18 
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase
Noda, Shuhei; Miyazaki, Takaya; Miyoshi, Takanori; Miyake, Michiru; Okai, Naoko; Tanaka, Tsutomu; Ogino, Chiaki; Kondo, Akihiko
. ...
... different plasmids were used in this study: the  pUC18 
Obtaining transgenic plants using the bio-active beads method
Obtaining transgenic plants using the bio-active beads method
Liu, Haibo; Kawabe, Akira; Matsunaga, Sachihiro; Murakawa, Tomoko; Mizukami, Atsushi; Yanagisawa, Masanobu; Nagamori, Eiji; Harashima, Satoshi; Kobayashi, Akio; Fukui, Kiichi
-sGFP (4.1-kb) plasmid containing a cauliflower ...
... with angD, constructed in  pET43.1a This work    pUC18 
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Gene cloning, expression and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1
Liu, Qin; Ma, Yue; Zhou, Lingyun; Zhang, Yuanxing
Cloning/expression vector Sangon (Shanghai, ...
... partially purified enzyme preparations using  pUC18 
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI
Gupta, Richa; Xu, Shuang-Yong; Sharma, Prince; Capalash, Neena
and/or λ DNA as substrates and Universal Buffer ...
... of STE2 encoding alpha-factor receptor, plasmid  pUC18 
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Liu, Jing-Jing; Ding, Wen-Tao; Zhang, Guo-Chang; Wang, Jing-Yu
-ste2-URA was constructed. ...
... sterile and fertile lines were cloned into the  pUC18 
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica
Das, Srirupa; Sen, Supriya; Chakraborty, Anirban; Chakraborti, Papia; Maiti, Mrinal K; Basu, Asitava; Basu, Debabrata; Sen, Soumitra K
vector. ...
... subcloned into a double-stranded vector such as  pUC18 
BAC Finishing Strategies
BAC Finishing Strategies
Bird, Christine; Grafham, Darren
or single-stranded M13 vector. ...
... K. pneumoniae were cloned into the  pUC18 
Synthesis of Pure meso-2,3-Butanediol from Crude Glycerol Using an Engineered Metabolic Pathway in Escherichia coli
Synthesis of Pure meso-2,3-Butanediol from Crude Glycerol Using an Engineered Metabolic Pathway in Escherichia coli
Lee, Soojin; Kim, Borim; Park, Kyungmoon; Um, Youngsoon; Lee, Jinwon
plasmid and introduced into E. coli. In 2 l ...
... as previously described [2] with either:  pUC18 
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia colistrains
Lee, David J; Bingle, Lewis EH; Heurlier, Karin; Pallen, Mark J; Penn, Charles W; Busby, Stephen JW; Hobman, Jon L
[17], 2,700 bp (high copy number plasmid), ...