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... the gene encoded in the plasmid substrates (  pUC18 
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan
: TS) for use in the cleavage reactions. (c) ...
... E. coli cloning vector, 4.3 kb TaKaRa     pUC18 
Role of DNA in Bacterial Aggregation
Role of DNA in Bacterial Aggregation
Liu, Hui-Hui; Yang, Yi-Ran; Shen, Xin-Cheng; Zhang, Zhi-Ling; Shen, Ping; Xie, Zhi-Xiong
E. coli cloning vector, 2.7 kb TaKaRa   Herring ...
... pDONR™ P4-P1R, and pDONR™ P2R-P3 (see Note 1),  pUC18 
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Methods for Constructing Clones for Protein Expression in Mammalian Cells
Sone, Takefumi; Imamoto, Fumio
(8), template DNA for amplification of ...
... of Escherichia coli DH5α cells harbouring  pUC18 
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
Matos, Tiago; Mohamed, Elsayed T.; Queiroz, João A.; Bülow, Leif
in LB medium [26]. ...
... with two XcmI cassettes was inserted into  pUC18 
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products
Gu, Jingsong; Ye, Chunjiang
to construct an efficient XcmI T-vector parent ...
... expressions of DR4 and DR5, we transfected the  pUC18 
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells
Kong, Fanyun; You, Hongjuan; Zhao, Jinjin; Liu, Wen; Hu, Lei; Luo, Wenya; Hu, Wei; Tang, Renxian; Zheng, Kuiyang
-HBV1.2, a plasmid contains the full-length HBV ...
... by standard PCR techniques from the template  pUC18 
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Proteolytic Cleavage of HIV-1 GFP-Vpr Fusions at Novel Sites Within Virions and Living Cells: Concerns for Intracellular Trafficking Studies
Caly, Leon; Jans, David A.; Piller, Sabine C.
-NL4.3 [15]. ...
... of the 3′-end region of the GPD1. The plasmid  pUC18 
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Overexpressing GLT1 in gpd1Δ mutant to improve the production of ethanol of Saccharomyces cerevisiae
Kong, Qing-Xue; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun
-R was created by inserting a 446-bp of ...
... to keep the acidity constant at pH 6.0. Plasmid  pUC18 
Biological assays and noncovalent interactions of pyridine-2-carbaldehyde thiosemicarbazonecopper(II) drugs with [poly(dA–dT)]2, [poly(dG–dC)]2, and calf thymus DNA
Biological assays and noncovalent interactions of pyridine-2-carbaldehyde thiosemicarbazonecopper(II) drugs with [poly(dA–dT)]2, [poly(dG–dC)]2, and calf thymus DNA
Ruiz, Rebeca; García, Begoña; Garcia-Tojal, Javier; Busto, Natalia; Ibeas, Saturnino; Leal, José M.; Martins, Célia; Gaspar, Jorge; Borrás, Joaquín; Gil-García, Rubén; González-Álvarez, Marta
(0.25 μg μL−1, 750 μM in nucleotides) in TE ...
... DNA in solution The interaction of HUTth with  pUC18 
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
HU histone-like DNA-binding protein from Thermus thermophilus: structural and evolutionary analyses
Papageorgiou, Anna C.; Adam, Panagiotis S.; Stavros, Philemon; Nounesis, George; Meijers, Rob; Petratos, Kyriacos; Vorgias, Constantinos E.
plasmid DNA was performed by MST (Zillner et ...
... K12 and the recombinant strain E. coli JM101  pUC18 
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Development of a generic approach to native metalloproteomics: application to the quantitative identification of soluble copper proteins in Escherichia coli
Sevcenco, Ana-Maria; Krijger, Gerard C.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Hagen, Wilfred R.; Hagedoorn, Peter-Leon
-azu [17] were used. ...
... antibody. Materials and methods Materials The  pUC18 
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
Hao, Chao-hui; Han, Qian-he; Shan, Zhong-jie; Hu, Jian-ting; Zhang, Nan; Zhang, Xue-pei
and pSectag2B plasmids, restriction ...
... the construction of the integration vector were  pUC18 
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Construction of engineered Arthrobacter simplex with improved performance for cortisone acetate biotransformation
Zhang, Huitu; Tian, Yao; Wang, Jianling; Li, Yonghai; Wang, Haikuan; Mao, Shuhong; Liu, Xiaoguang; Wang, Chunxia; Bie, Songtao; Lu, Fuping
and pUC57. ...
... intergenic region (Fig. 1A) was cloned into  pUC18 
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Samsonova, Natalya N; Smirnov, Sergey V; Altman, Irina B; Ptitsyn, Leonid R
vector and sequenced. ...
... et al. (1991a) pEBM2 8.0 pBL1 Kmr, lacZα  pUC18 
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Promoters and Plasmid Vectors of Corynebacterium glutamicum
Pátek, Miroslav; Nešvera, Jan
MCS Mobilizable Tauch et al. (1998) pMJ-Ab 5.2 ...
... vector harboring the gene X to be mutated:  pUC18 
Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
Mondon, Philippe; Grand, David; Souyris, Nathalie; Emond, Stéphane; Bouayadi, Khalil; Kharrat, Hakim
-X (cloned into BamHI, EcoRI restriction ...
... for AFM For AFM imaging in air, 100.0 μg/ml  pUC18 
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
A simple and effective sample preparation method for atomic force microscopy visualization of individual DNA molecules in situ
Shen, Xin-Cheng; Bao, Lei; Zhang, Zhi-Ling; Liu, Xiaoshen; Pang, Dai-Wen; Xu, Jianrong
DNA sample was diluted into final concentration ...
... E. coli (MG1655), this wild-type with the  pUC18 
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake
Gokarn, R.R.; Eiteman, M.A.; Altman, E.
cloning/expression vector (MG1655/ ...
... expression Datsenko and Wanner (2000)  pUC18 
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917
Yang, Ying; Yang, Yi; Ou, Bingming; Xia, Pengpeng; Zhou, Mingxu; Li, Luan; Zhu, Guoqiang
Ampr, cloning vector Lab stock ...
... and Chemicals pBluescript II KS (–),  pUC18 
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Identification and Characterization of sanH and sanI Involved in the Hydroxylation of Pyridyl Residue During Nikkomycin Biosynthesis in Streptomyces ansochromogenes
Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong
, and pIJ2925 were used for routine cloning and ...
... coli, such as those derived from pBR322 or  pUC18 
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
So, Lok Yan; Chen, Wen-yang; Lacap-Bugler, Donnabella C; Seemann, Myriam; Watt, Rory M
, cannot be stably-maintained in Z. ...
... and Methods Materials The 2686-bp-long  pUC18 
Evaluation of a Whole Genome Amplification Method Based on Improved Ligation-Mediated PCR
Evaluation of a Whole Genome Amplification Method Based on Improved Ligation-Mediated PCR
Pan, Xiaoming; Chen, Weikai; Jia, Xiushuang; Dong, Ping; Liang, Xingguo
plasmid was extracted from bacteria culture ...
... showed no products with the gDNA of Z. mays and  pUC18 
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety
Weihmann, Fabian; Eisermann, Iris; Becher, Rayko; Krijger, Jorrit-Jan; Hübner, Konstantin; Deising, Holger B.; Wirsel, Stefan G. R.
(Additional file 1A). ...
... Sisco Research Laboratories, Mumbai, India.  pUC18 
Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.)
Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.)
Ani, V.; Varadaraj, M. C.; Naidu, K. Akhilender
DNA was purchased from Bangalore Genei, ...
...   pKD4 Apr Kmr [18]   pETSPO Cmr MLSr [4]     pUC18 
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Croux, Christian; Nguyen, Ngoc-Phuong-Thao; Lee, Jieun; Raynaud, Céline; Saint-Prix, Florence; Gonzalez-Pajuelo, Maria; Meynial-Salles, Isabelle; Soucaille, Philippe
Apr Fermentas    ...