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... with BamHI and HindIII and cloned into plasmid  pUC18 
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Selection of a whole-cell biocatalyst for methyl parathion biodegradation
Yang, Jijian; Liu, Ruihua; Jiang, Hong; Yang, Yao; Qiao, Chuanling
to generate vector pUMb. ...
... the lacZ-tagged strain B. subtilis Tu-100(  pUC18 
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100
Hu, Xiaojia; Roberts, Daniel P.; Jiang, Mulan; Zhang, Yinbo
) was detected within all roots of oilseed ...
... Mannheim, Germany). Subcloning into  pUC18 
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7)
Debode, Frederic; Janssen, Eric; Berben, Gilbert
...
... pET28a to generate plasmid pET28a-bTE and into  pUC18 
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli
Liu, Xiping; Yu, Haiying; Jiang, Xu; Ai, Guomin; Yu, Bo; Zhu, Kun
to obtain plasmid ...
... sequentially constructed. The first plasmid  pUC18 
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
Kong, Qing-Xue; Gu, Ji-Guang; Cao, Li-Min; Zhang, Ai-Li; Chen, Xun; Zhao, Xue-Ming
-R was created by inserted a 446 bp of ...
... To do so, a homologous recombination plasmid  pUC18 
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites
Sun, Hengyi; Zang, Xiaonan; Liu, Yuantao; Cao, Xiaofei; Wu, Fei; Huang, Xiaoyun; Jiang, Minjie; Zhang, Xuecheng
-rDNA2-ura3-P pgk -5hsCT-rDNA1 was constructed, ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...
... of the target sequence than those produced in  pUC18 
Sequencing Bacterial Artificial Chromosomes
Sequencing Bacterial Artificial Chromosomes
Harris, David E.; Murphy, Lee
. ...
... 1.5 × 106 4 × 10−8 3 × 10−10 133.3 60Co γ–ray  pUC18 
DNA strand break dependence on Tris and arginine scavenger concentrations under ultra-soft X-ray irradiation: the contribution of secondary arginine radicals
DNA strand break dependence on Tris and arginine scavenger concentrations under ultra-soft X-ray irradiation: the contribution of secondary arginine radicals
Souici, Mounir; Khalil, Talat Tariq; Boulanouar, Omar; Belafrites, Abdelfettah; Mavon, Christophe; Fromm, Michel
29.75 ng µL−1 Tomita et al. (1995) 1.5 × 107 ...
... feces (serotype O1483: H1) ATCC Plasmids    pUC18 
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Secretory delivery of heterologous proteins in attenuated Vibrio anguillarum for potential use in vaccine design
Zhou, Lingyun; Liu, Qin; Wang, Qiyao; Ma, Yue; Xu, Yuzhou; Yang, Zhao; Zhao, Yan; Zhang, Yuanxing
Cloning/expression vector, Apr lacZ-α, Ori: ...
... and cells carrying either the empty vector  pUC18 
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong; Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... per recipient cell (t/r) using plasmid  pUC18 
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Rizzi, Aurora; Brusetti, Lorenzo; Arioli, Stefania; Nielsen, Kaare M.; Tamagnini, Isabella; Tamburini, Alberto; Sorlini, Claudia; Daffonchio, Daniele
or a 1,873 bp fragment as donor DNA, and 3.5 × ...
... Culture Collection (CICC). The plasmid  pUC18 
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Improved Lactose Utilization by Overexpression β-Galactosidase and Lactose Permease in Klebsiella pneumoniae
Guo, Xuewu; Wang, Yazhou; Guan, Xiangyu; Chen, Yefu; Zhang, Cuiying; Xiao, Dongguang
 K were provided by Yu Zheng, associate ...
... of the IPTG-inducible promoter, AAEC189(  pUC18 
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)
Dai, Jianjun; Wang, Shaohui; Guerlebeck, Doreen; Laturnus, Claudia; Guenther, Sebastian; Shi, Zhenyu; Lu, Chengping; Ewers, Christa
:aatA+P) expressing aatA under the control of ...
... The libraries were developed in two vectors,  pUC18 
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Mining the metagenome of activated biomass of an industrial wastewater treatment plant by a novel method
Sharma, Nandita; Tanksale, Himgouri; Kapley, Atya; Purohit, Hemant J.
and pBeloBAC11, and efficacy was demonstrated ...
... expression profile of pIAAD3 merB in pQE30 and  pUC18 
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Molecular Cloning and Genetic Analysis of Functional merB Gene from Indian Isolates of Escherichia coli
Murtaza, Imtiyaz; Dutt, Amit; Mushtaq, Dhuha; Ali, Arif
vectors each demonstrated 22.2 kDa proteins. ...
... RRS (IRMM-410R), and from the cloning vector  pUC18 
Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays)
Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays)
Rønning, Sissel B.; Vaïtilingom, Marc; Berdal, Knut G.; Holst-Jensen, Arne
(Amersham Biotech) were used as templates in ...
... Biotinylated oligo-d T18: 5′[biotin]dT18 3′. 8.  pUC18 
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
Jansen, Bastiaan J. H.; Jongh, Gys; Schalkwijk, Joost; Ruissen, Fred
Forward: 5′ AAG TTG GGT AAC GCC AGG 3′. 9. ...
... and inserted into the BamHI and SalI sites of  pUC18 
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
Yong Kang, C.
. ...
... pSVJ21, was constructed by ligating p54 and  pUC18 
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients
Miteva, Vanya; Lantz, Sarah; Brenchley, Jean
and inserting a chloramphenicol acetyl ...
... into the SacI and SalI enzyme-cutting sites of  pUC18 
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Molecular cloning, expression and purification of recombinant soluble mouse endostatin as an anti-angiogenic protein in Escherichia coli
Yari, Kheirollah; Afzali, Saboor; Mozafari, Hadi; Mansouri, Kamran; Mostafaie, Ali
cloning vector. ...
... DNA from plasmid  pUC18 
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Shiina, Takuya; Watanabe, Ritsuko; Shiraishi, Iyo; Suzuki, Masao; Sugaya, Yuki; Fujii, Kentaro; Yokoya, Akinari
was irradiated with low-LET (13 keV/μm) or ...
... was conducted as previously described [17, 27].  pUC18 
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7
Jeong, Kwang Cheol; Hung, Kai Foong; Baumler, David J; Byrd, Jeffrey J; Kaspar, Charles W
plasmid DNA (300 ng) was mixed with varying ...
... transformed pEC into E. coli JM83 harboring  pUC18 
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... the gene encoded in the plasmid substrates (  pUC18 
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes
Kandavelou, Karthikeyan; Chandrasegaran, Srinivasan
: TS) for use in the cleavage reactions. (c) ...