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... 2 Indirect Antigen; antibody; antispecies  conjugate 
Titration of Reagents
Titration of Reagents
Crowther, John R.
3 Direct sandwich Capture antibody; ...
... for 2 h, wash the plate and then dilute the  conjugate 
Practical Exercises
Practical Exercises
Crowther, John R.
across the plate, the opposite way to the ...
... available in higher amounts. The antimouse  conjugate 
Monoclonal Antibodies
Monoclonal Antibodies
Crowther, John R.
used must detect all isotypes of mouse IG ...
... with their specific substrates. Enzyme  conjugate 
Systems in ELISA
Systems in ELISA
Crowther, John R.
An enzyme that is attached irreversibly to ...
... capacity of the antibody-catalase  conjugate 
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
Muzykantov, Vladimir R.
in vitro, and characterization of ...
... 10-µL volume for preparation of working  conjugate 
Stages in ELISA
Stages in ELISA
Crowther, John R.
dilutions requires the best accuracy and ...
... Use this pipeter for antibody and ABA-BSA  conjugate 
Abscisic Acid
Abscisic Acid
Walker-Simmons, M. K.; Rose, Patricia A.; Hogge, Lawrence R.; Abrams, Suzanne R.
solutions. ...
... observed in addition to the RNase-antibody  conjugate 
Construction of Ribonuclease–Antibody Conjugates for Selective Cytotoxicity
Construction of Ribonuclease–Antibody Conjugates for Selective Cytotoxicity
Newton, Dianne L.; Rybak, Susanna M.
(see Note 22). ...
... titration of antigen against a specific  conjugate 
Test Questions
Test Questions
Crowther, John R.
. ...
... isothiocyanate (FITC)-labeled rabbit IgG  conjugate 
Immunohistochemistry in Research and Diagnosis of Celiac Disease
Immunohistochemistry in Research and Diagnosis of Celiac Disease
Brandtzaeg, Per; Jahnsen, Frode L.
specific for human IgA (1/30; DAKO) for 30 ...
... are observed, and to wells in which is only  conjugate 
Charting Methods for Internal Quality Control
Charting Methods for Internal Quality Control
Crowther, John R.
is added (Cc) where low color is observed. ...
... chemistry, which leads to lower cost of  conjugate 
Folate as a Targeting Device for Proteins Utilizing Folate Receptor-Mediated Endocytosis
Folate as a Targeting Device for Proteins Utilizing Folate Receptor-Mediated Endocytosis
Lee, Robert J.; Low, Philip S.
production and easier quality control; and ...
... a viable assay a different protein: hapten  conjugate 
Antibodies for Immunoassays
Antibodies for Immunoassays
Newman, David J.
will be required from that used as an ...
... 4. Stringent wash buffer. 5. Concentrated  conjugate 
Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Stuyver, Lieven; Wyseur, Ann; Verpooten, Gonda; Rossau, Rudi
(to be diluted l∶100 in ...
... ELISA 1. Antimouse alkaline phosphatase  conjugate 
Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein
Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein
Gray, Carol W.; Karran, Eric H.
(Sigma, cat. no. A9316). 2. Fish gelatin ...
... was treated with iv injections of OX-26-NGF  conjugate 
Trophic Influences on Neural Tissue Transplants
Trophic Influences on Neural Tissue Transplants
Granholm, Ann-Charlotte
for 3 mo; in (B), a control transplant is ...
... the surface of colloidal gold, so when the  conjugate 
Immunolabeling for Electron Microscopy
Immunolabeling for Electron Microscopy
Sarraf, Catherine E.
locates the primary antibody, gold grains ...
... no. S026-220). 5. Alkaline phosphatase (AP)  conjugate 
Whole Gut Lavage Fluid Analysis
Whole Gut Lavage Fluid Analysis
Ghosh, Subrata; Dahele, Anna; Drummond, Hazel E.; Hoque, Syed S.; Humphreys, Kenneth; Arnott, Ian D. R.
: Goat antihuman IgG (Sigma; cat. no. ...
... MO, USA). Direct Method Anti-hapten gold-  conjugate 
In Situ Hybridization for Electron Microscopy
In Situ Hybridization for Electron Microscopy
Waller, Ross F.; McFadden, Geoffrey I.
. ...
... of Haemophilus influenzae type b  conjugate 
Timing of Vaccinations in Premature Infants
Timing of Vaccinations in Premature Infants
D’Angio, Carl T.; Hall, Caroline B.
vaccines varies widely in studies of ...
... that are used to prepare the targeted  conjugate 
Antibody Conjugation Methods for Active Targeting of Liposomes
Antibody Conjugation Methods for Active Targeting of Liposomes
Ansell, Steven M.; Harasym, Troy O.; Tardi, Paul G.; Buchkowsky, Susan S.; Bally, Marcel B.; Cullis, Pieter R.
. ...
... antibody/alkaline phosphatase  conjugate 
Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence
Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence
Wheeler, Claire B.; Harvey, Bronwen M.; Cunningham, Martin
. ...
... methanol. 4. Streptavidin-peroxidase (POD)  conjugate 
Nonradioactive Methods for the Detection of RNA-Protein Interaction
Nonradioactive Methods for the Detection of RNA-Protein Interaction
Echarri, Asier; González, María Eugenia; Ventoso, Iván; Carrasco, Luis
at 500 U/mL (Boehringer Mannheim, Mannheim, ...
... adverse events among those who received Hib  conjugate 
Safety of Routine Childhood Vaccinations
Safety of Routine Childhood Vaccinations
Chen, Robert T.; Mootrey, Gina; DeStefano, Frank
vaccine and those who received ...
... antigens in indirect ELISA procedures is to  conjugate 
Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
Murray, Andrea; O’Sullivan, Deirdre A.; Price, Michael R.
the peptides to a large carrier protein ...