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... of the practical steps are similar. The  conjugates 
Practical Exercises
Practical Exercises
Crowther, John R.
used are all horseradish peroxidase (HRP) ...
... Number titrated Direct Antigen; antibody  conjugated 
Titration of Reagents
Titration of Reagents
Crowther, John R.
to enzyme 2 Indirect Antigen; antibody; ...
... of mAbs as capture reagent; examination of  conjugates 
Monoclonal Antibodies
Monoclonal Antibodies
Crowther, John R.
5. ...
... an affinity, catalase could be chemically  conjugated 
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
14 Immunotargeting of Catalase to the Pulmonary Vascular Endothelium
Muzykantov, Vladimir R.
with a carrier antibodies recognizing the ...
... with their specific substrates. Enzyme  conjugate 
Systems in ELISA
Systems in ELISA
Crowther, John R.
An enzyme that is attached irreversibly to ...
... 10-µL volume for preparation of working  conjugate 
Stages in ELISA
Stages in ELISA
Crowther, John R.
dilutions requires the best accuracy and ...
... Chapter 6 , this volume) and chemical RNase  conjugates 
Construction of Ribonuclease–Antibody Conjugates for Selective Cytotoxicity
Construction of Ribonuclease–Antibody Conjugates for Selective Cytotoxicity
Newton, Dianne L.; Rybak, Susanna M.
have been made and characterized. ...
... Use this pipeter for antibody and ABA-BSA  conjugate 
Abscisic Acid
Abscisic Acid
Walker-Simmons, M. K.; Rose, Patricia A.; Hogge, Lawrence R.; Abrams, Suzanne R.
solutions. ...
... 2.1.3. ) 2. Apply paired fluorochrome  conjugates 
Immunohistochemistry in Research and Diagnosis of Celiac Disease
Immunohistochemistry in Research and Diagnosis of Celiac Disease
Brandtzaeg, Per; Jahnsen, Frode L.
in various combinations ( Table 3 ) of ...
... Receptor-Mediated Endocytosis of Folate  Conjugates 
Folate as a Targeting Device for Proteins Utilizing Folate Receptor-Mediated Endocytosis
Folate as a Targeting Device for Proteins Utilizing Folate Receptor-Mediated Endocytosis
Lee, Robert J.; Low, Philip S.
The folate receptor facilitates the ...
... titration of antigen against a specific  conjugate 
Test Questions
Test Questions
Crowther, John R.
. ...
... (9). Most studies have focused on antibody  conjugates 
Antibody Conjugation Methods for Active Targeting of Liposomes
Antibody Conjugation Methods for Active Targeting of Liposomes
Ansell, Steven M.; Harasym, Troy O.; Tardi, Paul G.; Buchkowsky, Susan S.; Bally, Marcel B.; Cullis, Pieter R.
since procedures for producing highly ...
... are observed, and to wells in which is only  conjugate 
Charting Methods for Internal Quality Control
Charting Methods for Internal Quality Control
Crowther, John R.
is added (Cc) where low color is observed. ...
... a viable assay a different protein: hapten  conjugate 
Antibodies for Immunoassays
Antibodies for Immunoassays
Newman, David J.
will be required from that used as an ...
... ELISA 1. Antimouse alkaline phosphatase  conjugate 
Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein
Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein
Gray, Carol W.; Karran, Eric H.
(Sigma, cat. no. A9316). 2. Fish gelatin ...
... Jousselin-Hosaja, 1993 NGF/septal tissue  Conjugated 
Trophic Influences on Neural Tissue Transplants
Trophic Influences on Neural Tissue Transplants
Granholm, Ann-Charlotte
to OX-26, iv Friden et al., 1993 GDNF/VM tp ...
... of interest, and a secondary antibody,  conjugated 
Immunolabeling for Electron Microscopy
Immunolabeling for Electron Microscopy
Sarraf, Catherine E.
to an electron dense moiety, is directed ...
... no. S026-220). 5. Alkaline phosphatase (AP)  conjugate 
Whole Gut Lavage Fluid Analysis
Whole Gut Lavage Fluid Analysis
Ghosh, Subrata; Dahele, Anna; Drummond, Hazel E.; Hoque, Syed S.; Humphreys, Kenneth; Arnott, Ian D. R.
: Goat antihuman IgG (Sigma; cat. no. ...
... MO, USA). Direct Method Anti-hapten gold-  conjugate 
In Situ Hybridization for Electron Microscopy
In Situ Hybridization for Electron Microscopy
Waller, Ross F.; McFadden, Geoffrey I.
. ...
... in which MUC1 immunodominant peptides ware  conjugated 
Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
Murray, Andrea; O’Sullivan, Deirdre A.; Price, Michael R.
to a polyl-ysine backbone (7). ...
... (MAbs) and reporter enzyme Ni-NTA  conjugates 
Detection and Immobilization of Proteins Containing the 6xHis Tag
Detection and Immobilization of Proteins Containing the 6xHis Tag
Kneusel, Richard E.; Wulbeck, Melanie; Ribbe, Joachim
, both of which can detect histidine-tagged ...
... 4. Stringent wash buffer. 5. Concentrated  conjugate 
Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase
Stuyver, Lieven; Wyseur, Ann; Verpooten, Gonda; Rossau, Rudi
(to be diluted l∶100 in ...
... women with the bovine serum albumin (BSA)-  conjugated 
Detection of Humoral Immune Responses to Mucins
Detection of Humoral Immune Responses to Mucins
Mensdorff-Pouilly, Silvia; Vennegoor, Claus; Hilgers, Joseph
peptide assay (described under Subheading ...
... steps. First, the two cells form random  conjugates 
Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis
Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis
Valitutti, Salvatore; Dessing, Mark
mediated by nonspecific interactions ...
... of Haemophilus influenzae type b  conjugate 
Timing of Vaccinations in Premature Infants
Timing of Vaccinations in Premature Infants
D’Angio, Carl T.; Hall, Caroline B.
vaccines varies widely in studies of ...