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... 2 h, wash the plate, and then dilute the  conjugate 
Direct ELISA
Direct ELISA
Crowther, John R.
across the plate, the opposite way to the ...
... antibody, the detecting antibody, and the  conjugate 
Use of Antibodies on Solid-Phase in Capture ELISA
Use of Antibodies on Solid-Phase in Capture ELISA
Crowther, John R.
are used at optimal dilutions. ...
... since only a single antispecies enzyme  conjugate 
Basic Principles of ELISA
Basic Principles of ELISA
Crowther, John R.
is needed to titrate antisera from many ...
... can be used since the AB is labeled with  conjugate 
Competitive ELISA
Competitive ELISA
Crowther, John R.
. ...
... that have been in contact with any enzyme  conjugate 
Stages in ELISA
Stages in ELISA
Crowther, John R.
be recycled, and these should not be ...
... with an antigen by using an antispecies  conjugate 
Indirect ELISA
Indirect ELISA
Crowther, John R.
. ...
... can be overcome by repurifying the antibody  conjugate 
Coupling of Monoclonal Antibodies with Fluorophores
Coupling of Monoclonal Antibodies with Fluorophores
Haugland, Rosaria P.
by gel-filtration chromatography before ...
... should produce a good yield of stable  conjugate 
Coupling of Monoclonal Antibodies with Enzymes
Coupling of Monoclonal Antibodies with Enzymes
Haugland, Rosaria P.
with minimal loss of enzymatic and ...
... that serve to bind an antibody-enzyme  conjugate 
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
Groody, E. Patrick
to the immobilized probe-target complex. ...
... biotinylated protein) and the avidin-probe  conjugate 
Coupling of Monoclonal Antibodies with Biotin
Coupling of Monoclonal Antibodies with Biotin
Haugland, Rosaria P.; You, Wendy W.
, where the probe can be a fluorochome or ...
... The assay utilizes a substrate-carrier  conjugate 
Screening Strategies for Catalytic Antitransition-State Analog Antibodies
Screening Strategies for Catalytic Antitransition-State Analog Antibodies
Tramontano, Alfonso
as the immunosorbent and requires a ...
... agent used, once the antibody-DTPA  conjugate 
Radiolabeling of Antibodies for Therapy and Diagnosis
Radiolabeling of Antibodies for Therapy and Diagnosis
Baranowska-Kortylewicz, Janina; Dalrymple, Glenn V.; Quadri, Syed M.; Harrison, Katherine A.
is purified, radiolabeling protocols are ...
... Tween-20. 15. Antimouse λ chain-biotin  conjugate 
Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation
Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation
Deng, Su-jun; MacKenzie, C. Roger; Narang, Saran A.
(Caltag, San Francisco, CA), diluted ...
... that binds DalB02) it was observed that one  conjugate 
Inhibition of Epstein-Barr-virus-transformed human chronic lymphocytic leukaemic B cells with monoclonal-antibody-Adriamycin (doxorubicin) conjugates
Inhibition of Epstein-Barr-virus-transformed human chronic lymphocytic leukaemic B cells with monoclonal-antibody-Adriamycin (doxorubicin) conjugates
Zhu, Zhenping; Kralovec, Jaroslav; Ghose, Tarunendu; Mammen, Molly
was more potent than the free drug but the ...
... antigen, an enzyme-labeled antibody (  conjugate 
Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens
Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens
Robison, Barbara J.
) is added, which also binds to the ...
... nonquadratic functions using the nonlinear  conjugate 
A Conjugate Gradient Algorithm with a Trust Region for Molecular Geometry Optimization
A Conjugate Gradient Algorithm with a Trust Region for Molecular Geometry Optimization
Bofill, Josep Maria
gradient method with a restricted step and ...
... monoclonal antibody C27-abrin A chain  conjugate 
Growth suppression of human colorectal carcinoma in nude mice by monoclonal antibody C27-abrin A chain conjugate
Growth suppression of human colorectal carcinoma in nude mice by monoclonal antibody C27-abrin A chain conjugate
Tsai, Lai-Chen; Chen, Yung-Liang; Lee, Chung; Chen, Horng-Min; Chang, Zo-Nan; Hung, Mei-Whey; Chao, Pei-Ling; Lin, Jung-Yaw
(MAAC), that might be effective in the ...
... the nonlocalised unbound antibody-enzyme  conjugate 
Antibody-Directed Enzyme Prodrug Therapy
Antibody-Directed Enzyme Prodrug Therapy
Knox, Richard J.; Connors, Torn A.
is allowed to clear naturally from the ...
... 2. A specified FAb (see Notes 1 and 2)  conjugate 
The Direct Immunofluorescent Filter Technique (DIFT)
The Direct Immunofluorescent Filter Technique (DIFT)
Kroll, Rohan
in a suitable buffer (e.g., PBS). 3. A ...
... antibody against the IgE receptor. (A) Gold  conjugate 
Pre-Embedding Labeling Methods
Pre-Embedding Labeling Methods
Oliver, Constance
is localized primarily in coated pits. ...
... investigator or obtained as a fluorescent  conjugate 
Assay for Chemoattractant Binding
Assay for Chemoattractant Binding
Harvath, Liana; Aksamit, Robert R.; Cunningham, Robert E.
from a commercial source) is evaluated in a ...
... second antibody and a streptavidin-HRP  conjugate 
Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection
Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection
Fowler, Susan J.
may be used in place of the HRP-labeled ...
... Wiener-Hopf equations by the preconditioned  conjugate 
Circulant integral operators as preconditioners for Wiener-Hopf equations
Circulant integral operators as preconditioners for Wiener-Hopf equations
Chan, Raymond H.; Jin, Xiao-Qing; Ng, Michael K.
gradient method. ...
... a chemically well-defined sugar-peptide  conjugate 
Bromoacetylated Synthetic Peptides
Bromoacetylated Synthetic Peptides
Robey, Frank A.
from Neisseria meningitidis as a synthetic ...
... York), or Extravidin-horseradish peroxidase  conjugate 
In situ hybridization to drosophila polytene chromosomes
In situ hybridization to drosophila polytene chromosomes
Saunders, Robert D. C.
(Sigma, St. ...